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Biomedical subjects

J Svejcar

Publications and source records attributed to J Svejcar.

At least 73 records · Page 4Linked to original sources
PubMed1975

The diagnosis of the early infantile form of hypophosphatasia tarda.

The diagnostic characteristics of the early infantile form of hypophosphatasia tarda are demonstrated in a case of a first child of healthy, probably consanguineous, unmarried parents. The diagnosis of the disease is based on the clinical and radiographic findings. The absence of alkaline phosphatase activity in the blood serum and leukocytes, and the excretion of phosphorylethanolamine in urine confirm the diagnosis. The inheritance appears to be autosomal recessive.

Alkaline Phosphatase↗
PubMed1975

Age-dependence of spontaneous delayed hypersensitivity to DNA, antinuclear antibody production and development of glomerulonephritis in NZB mice.

In NZB mice of different ages (2, 4, 8, 12, and 16 months) the positivity of the migration inhibition test performed with the ds-DNA in spleen explants was compared with the level of immunofluorescent antinuclear antibodies and the incidence of glomerulonephritis. The positivity of the MIT was apparent at 4 months of age, rose with ageing and decreased after 12 months of age. Production of ANA began later, mostly at 8 months of age, increased progressively with ageing, especially in female mice, and was followed by the development of glomerulonephritis which reached its peak at 16 months of age.

Age Factors↗
PubMed1975

Further characterization of antigen-dependent migration inhibition factor in mice.

Antigen-dependent MIF was produced in inbred mice. Lymph node cells from mice sensitized to tubercle bacilli were incubated with small quantities of antigen. The supernatant contained antigen-dependent MIF and its activity when tested on mouse and rabbit spleen was minimal unless PPD was added to the supernatant. The production of the antigen-dependent MIF was T cell-dependent, as shown by the use of anti-theta serum. Its molecular weight was in the range of 50--100,000 and it was concluded that the factor is different from conventional antibodies or antigen-antibody complexes.

Animals↗
PubMed1975

In vitro production of the factors effective in the transfer of experimental autoimmune aspermatogenesis.

We studied the biological effects of the factors released into the culture medium by the lymphocytes from animals sensitized with testicular antigen. The supernatants from cultures of these lymphocytes were active in vitro in the migration inhibition tests because they transferred the sensitivity to normal spleen cells. In vivo, they transferred aspermatogenesis in the allogeneic and xenogeneic system. The results suggest a specific effect on the behaviour of macrophages rather than a direct cytotoxic effect of the active factors.

Animals↗
PubMed1975

Prevention of spontaneous autoimmunity to DNA in NZB/Swiss mice by treatment with natural double-stranded RNA.

Data are presented demonstrating the prevention of spontaneous autoimmune disease in NZB/Swiss mice treated natural double-stranded RNA. The successful treatment of animals was followed both by improvement in clinical manifestations and by the migration inhibition test performed with DNA as antigen. The reasons for the successful employment of natural double-stranded RNA are thought to be in its physicochemical properties.

Animals↗
PubMed1975

Correlation between the results of the migration inhibitory factor production test with DNA and the severity of the disease in the systemic lupus erythematosus patients.

A correlation was made between the severity of clinical symptoms of SLE and the results of the migration inhibitory factor (MIF) production test. For this purpose 10 patients with various manifestations of SLE were examined by MIF production test before and after the immunosuppressive therapy. A good correlation between the actual clinical stage and the results of the MIF production test was found. The successful immunosuppressive therapy turned the positive MIF production into the negative one in most of the observed patients. The MIF production test is therefore recommended as a good complementary test for the estimation of the actual state of the SLE.

Antigens↗
PubMed1975

Autoimmunity as possible evoking factor of some so-called idiopathic bone marrow hypoplasias.

Patients with bone marrow hypoplasia or aplasia were examined using the method of lymphocyte blast transformation and the MIF production test. The experimental results demonstrated the presence of an autoimmune process in these patients. A good congruence of the methods was demonstrated. Possible role of the autoimmune process in the pathogenesis of the disease as well as the reasons for the autoimmunity development and the therapeutical implications of these findings are discussed.

Autoantibodies↗
PubMed1975

Spontaneous occurrence of delayed hypersensitivity to DNA in old NZB mice.

The sensitivity of NZB/Bl and NZB/Swiss Fl hybride mice spleen lymphocytes to DNA was examined using the MIF production and inhibition of migration from artificial fragment. The positivity was found in the group of old mice which was considered as an indication of spontaneous autoimmune process. The possible mechanism of this process is discussed.

Aging↗
PubMed1975

Specific acquired resistance and activity of migration inhibition factor (MIF) in spleens of mice with chronic tuberculosis.

The antimycobacterial resistance of the host (as evaluated by mycobacterial counts in spleens) and the in vitro cell-mediated immunological mechanisms ((cellular immune reactivity) assayed by the activity of migration inhibition factor in splenic fragments) were compared in the course of experimental chronic tuberculosis infection. Mice, either unvaccinated or vaccinated with killed mycobacteria suspended in saline and then either untreated or treated with azathioprine, were used in the study. The treatment with azathioprine caused a relative depression of specific resistance accompanied by less pronounced inhibition of migration in vitro. Although a higher level of specific resistance was detected in mice vaccinated with non-living vaccine, no enhancement of cellular reactivity in vitro could be estimated in the splenic cells of these animlas. The participation of humoral mechanisms in mice vaccinated with the non-living vaccine plausibly explains the discrepancy. The results indicate furthermore, that the migration inhibition test reflects delayed hypersensitivity rather than other components of cellular immune reactivity.

Animals↗