Search PubMed⌕ Search

Biomedical subjects

J Svejcar

Publications and source records attributed to J Svejcar.

At least 19 recordsLinked to original sources
PubMed1993

Developmental changes in neutral glycosphingolipids of mouse placenta.

The mammalian placenta is a unique organ for the study of developmental changes. Placentas of laboratory animals such as the mouse allow for the determination of the exact stage of pregnancy, which cannot be achieved with human placenta. In this study, neutral glycosphingolipids were isolated from mouse (inbred strain C57BL/6) placentas, from day 10 to day 18 of gestation, and were separated by high performance thin layer chromatography. Densitometric measurements after orcinol staining showed, at day 10 of gestation, the presence of mono-, tetra-, tri- and dihexosylceramide in decreasing quantities, as well as four unidentified spots. On day 12, the glycosphingolipid composition changed with the disappearance of the unidentified spots and the appearance of an orcinol positive migrating similarly to the Forssman antigen; no further changes occurred between days 12 and 18 of gestation. The identity of the Forssman-like glycosphingolipid with the Forssman antigen was established by binding of 125I labelled Helix pomatia agglutinin (alpha-GalNAc specific) to glycosphingolipids separated on high performance thin layer chromatography plates, and by the reaction of the isolated glycosphingolipid with a monoclonal anti-Forssman antibody. The appearance of the Forssman antigen at day 12 of gestation coincided with the day of final maturation of the mouse placenta and subsequent cessation of growth, suggesting a possible role of the glycosphingolipid during embryonic development.

Animals↗
PubMed1987

[New possibilities of heterozygote detection of hemophilia A].

Hemophilia A is the most common inherited bleeding disorder in man. The recent isolation of the hemophilia gene has led to the identification of an intragenic restriction fragment length polymorphism (RFLP) which can be used for segregation analysis in families at risk for carrying the disease. In addition, a tightly linked extragenic RFLP can also be used for these analyses. In this paper, we exemplify the usefulness of DNA analysis in genetic counseling of families at risk for hemophilia A. Although DNA analysis allows carrier detection in the majority of families, bioassays are still required for accurate diagnosis when DNA analysis is not informative.

DNA↗
PubMed1984

Clinical and immunological characterization of systemic lupus erythematosus in patients with circulating interferon and migration inhibitory factor.

The simultaneous occurrence of interferon and migration inhibitory factor was found in sera from 9 patients with systemic lupus erythematosus (SLE). Their clinical picture was characterized by a moderate course of the disease. In 7 patients the inflammatory tendoarticular manifestations were accentuated. Glomerulonephritis was found only in 3 patients and was successfully suppressed by immunosuppressive therapy. In the peripheral blood of most of these patients, normal or moderately increased levels of active E-rosettes were found. Therefore we consider these SLE patients as a group with characteristic signs of activated cell-mediated immunity (lymphokine release, active E-rosettes). Although the reason for this activation remains unknown, we suppose that some concomitant bacterial infection, perhaps mycobacterial, may contribute to this immunological phenomenon.

Adult↗
PubMed1984

Effect of bilirubin on an in vitro correlate of cell-mediated immunity--the migration inhibition test.

The effect of bilirubin on one of the basic cell-mediated immunity reactions, i.e. the migration inhibition test was assessed. For this purpose the model of tuberculin hypersensitivity in rabbits was employed and the effect of bilirubin was studied on the migration inhibition test and its two main phases; the process of MIF production and the effect of already preformed MIF on the effector system. It was found that the neutralizing effect of bilirubin on the migration inhibition (caused by specific antigen) was due to the effect of bilirubin on the process of MIF production.

Animals↗
PubMed1984

HLA and obesity.

A possible association between HLA and obesity was investigated in 42 unrelated obese children from Hessen, Germany. A family study was possible in 11 families with a total of 26 children. No significant deviation was observed for one of the HLA ABC antigen frequencies in the sample group compared with controls (n = 220). The linkage analysis in families: a) ruled out a close linkage (less than 10 cM) between HLA and a hypothetical obesity gene (assumption of a dominant mode of inheritance), b) did not exclude recessive inheritance so far, since the importance of penetrance is unknown and a simple gene model is improbable, c) yielded also negative lod scores (which did not reach the elimination level of -2) for an intermediary genetic model.

Adult↗