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Biomedical subjects

J Su

Publications and source records attributed to J Su.

At least 109 records · Page 6Linked to original sources
PubMed1997

[Effect of huoxue tablet on the rheology of erythrocyte in primary hypertension].

OBJECTIVE: To explore the effect of Huoxue Tablet on the rheology of erythrocyte and the therapeutic effect in treating primary hypertension. METHODS: Seventy-five cases of primary hypertension patients were randomly divided into 3 groups. Group I was treated by Huoxue Tablet, group II was treated by composite Danshen tablet, group III was treated by placebo. Erythrocytes deformability and aggregation were determined by laser diffractometry apparatus before and after treatment. RESULTS: After treated by Huoxue Tablet for 5-6 weeks. The authors found that deformability of erythrocyte was increased, the aggregation of erythrocyte, the whole blood viscosity and the blood pressure were reduced. The clinical symptoms were relieved. CONCLUSIONS: Huoxue Tablet can obviously improve the rheology of erythrocyte of the primary hypertension and relieve the clinical symptoms. It can be used as a supplementary medicine in treating primary hypertension.

Adult↗
PubMed1997

[Studies on the chemical constituents of the alga Sargassum carpophyllum J. Agardh].

The alga Sargassum carpophyllum J. Agardh collected from the South China Sea was immersed in 95% ethyl alcohol. The combined extract was partitioned between ethyl acetate and water. The ethyl acetate phase was subjected to pressure-reduced chromatography over Silica H, eluted with a step gradient of EtOAc in petroleum ether. Two main fractions of sterols (I) and fatty acids (II) were obtained. The composition of I and II were identified by GC/MS. The fraction Ia was obtained, as a main composition of I, by repeated flash chromatography. On the basis of spectroscopic analysis, Ia was identified to be fucosterol.

Fatty Acids↗
PubMed1997

Corneal melting after avulsion of a Molteno shunt plate.

PURPOSE: We report a case of Molteno shunt avulsion after blunt trauma resulting in corneal melting. The implant's connecting tube had been placed above the superior rectus muscle. METHODS: Case report. RESULTS: After blunt trauma, the secondary plate of a double-plated Molteno implant became completely dislodged anteriorly onto the patient's cornea. The corneal tissue entrapped beneath the avulsed plate was found to have extensive melting requiring emergency explantation of the Molteno implant and corneal grafting. CONCLUSIONS: Corneal melting after Molteno shunt avulsion is a novel finding. Placing a double-plated Molteno implant's connecting tube under the superior rectus muscle might decrease the risk of shunt avulsion after trauma.

Adult↗
PubMed1997

[Correction of occipito-posterior position by maternal posture during the process of labor].

OBJECTIVE: To investigate the effect on correction of occipito-posterior (OP) presentation by changing maternal posture during labor. METHOD: A prospective study was conducted in 120 pregnant women from March 1994 to December 1995. Women in labor were instructed to take the lateral posture so that the resultant force of the gravity of the fetus, the buoyancy of amniotic fluid, and the intermittent uterine contraction may change the fetal position from occipito-posterior into occipito-anterior (OA) presentation. Another 120 women were selected as controls. RESULTS: (1) In the study group, 106 women (88.3%) delivered vaginally with fetal presentation changed from OP into OA, and 14 (11.7%) received cesarean section. In the control group, only 20 women (16.7%) delivered vaginally, and 100 (83.3%) had cesarean sections (P < 0.001). (2) The average time interval in the study group was 302.6 min for the first stage and 59.8 min for the second stage, whereas 483.7 min and 156.1 min respectively in the controls. A significant difference was noted (P < 0.01). CONCLUSION: It is an effective method for the mother to take the lateral posture on the same side of the fetal spine for correcting the OP position. The incidence of dystocia may be reduced as well as the cesarean section rate. The method in simple and effective, and can be used in most of hospitals.

Adult↗
PubMed1997

[Differentiation of benign and malignant palpable breast masses using 99mTc-MIBI imaging].

Forty-two patients with palpable breast masses were examined by 99mTc-MIBI imaging. All the results were compared with ultra-red scanning and B-ultrasongraphy. By pathological examination 26 out of 42 cases had breast carcinoma, 16 patients benign lesions. The sensitivity of 99mTc-MIBI imaging was 92.3%, the specificity was 96.0% and the accuracy 92.8%, which were higher than those of ultra-red scanning and B-ultrasound (P < 0.01). The authors consider that 99mTc-MIBI breast imaging is useful in distinguishing malignancies from benign breast masses.

Adenoma↗
PubMed1996

Association between receptor protein-tyrosine phosphatase RPTPalpha and the Grb2 adaptor. Dual Src homology (SH) 2/SH3 domain requirement and functional consequences.

Receptor protein-tyrosine phosphatase RPTPalpha is found associated in vivo with the adaptor protein Grb2. Formation of this complex, which contains no detectable levels of Sos, is known to depend on a C-terminal phosphorylated tyrosine residue (Tyr798) in RPTPalpha and on the Src homology (SH) 2 domain in Grb2 (, ). We show here that association of Grb2 with RPTPalpha also involves a critical function for the C-terminal SH3 domain of Grb2. Furthermore, Grb2 SH3 binding peptides interfere with RPTPalpha-Grb2 association in vitro, and the RPTPalpha protein can dissociate the Grb2-Sos complex in vivo. These observations constitute a novel mode of Grb2 association and suggest a model in which association with a tyrosine-phosphorylated protein restricts the repertoire of SH3 binding proteins with which Grb2 can simultaneously interact. The function of the Tyr798 tyrosine phosphorylation/Grb2 binding site in RPTPalpha was studied further by expression of wild type or mutant RPTPalpha proteins in PC12 cells. In these cells, wild type RPTPalpha interferes with acidic fibroblast growth factor-induced neurite outgrowth; this effect requires both the catalytic activity and the Grb2 binding Tyr798 residue in RPTPalpha. In contrast, expression of catalytically active RPTPalpha containing a mutated tyrosine phosphorylation/Grb2 association site enhances neurite outgrowth. Our observations associate a functional effect with tyrosine phosphorylation of, and ensuing association of signaling proteins with, a receptor protein-tyrosine phosphatase and raise the possibility that RPTPalpha association may modulate Grb2 function and vice versa.

Adaptor Proteins, Signal Transducing↗
PubMed1996

A novel ribozyme target site located in the HIV-1 nef open reading frame.

We have tested the sequence UUC CAG UCA GAC CU, at position 9016--9029 within the HIV-1(SF2) nef open reading frame, for accessibility to antisense and hammerhead ribozyme attack. The accessibility was first studied using a phosphorothioate-modified 14-nt DNA oligo (complementary to the nef9016--9029 site). A dose-dependent repression of HIV-1(SF2) growth was observed in human peripheral blood mononuclear cells after exogenous administration of the oligo to the cell culture medium. A hammerhead ribozyme with a 6+7-nt antisense specificity for the nef9016--9029 site (hhRz.nef9016--9029) was constructed and transfected into the human T-cell line HUT78. Again, a dose-dependent repression of virus growth was observed when different individual clones expressing hhRz.nef9016--9029 were infected with HIV-1(SF2). A complete abrogation of virus production was observed after infection with a low (0.5 TCID50) HIV-1 titer. Increasing doses (2.5 and 12.5 TCID50) of HIV-1 virus yielded a low production (10(3)-fold reduced) of virus particles in most cases; but a complete, or close to complete, abrogation was observed even in individual cultures infected with the highest dose. Presence of proviral pol and gag sequences in hhRz.nef9016--9029-expressing HUT78 clones was assayed, using PCR. Interestingly, since no pol and gag PCR products could be detected, the results strongly indicated that the hammerhead ribozyme was already acting on the infecting HIV RNA before its reverse transcription and integration as proviral DNA. In summary, the results obtained in this study support the nef9016--9029 site as a strong new candidate for ribozymal gene therapy against HIV-1 infection.

Base Sequence↗
PubMed1996

Postoperative symptoms 24 hours after ambulatory anaesthesia.

PURPOSE: To test the hypothesis that the type of surgical procedure influences the incidence of postoperative symptoms. Also the effect of demographic and clinical risk variables: age, sex, ASA status, duration of anaesthesia on the postoperative symptoms were evaluated for each type of surgery. METHODS: Demographic, medical, anaesthetic and surgical data on 1,017 patients were prospectively collected by a research assistant who telephoned each patient 24 hr after discharge to administer a questionnaire to determine postoperative symptoms. Postoperative symptoms included incisional pain, nausea/vomiting, drowsiness, dizziness, headache and fever. In addition, 270 patients were asked the % (0-100) of their return to daily living function at 24 hr. RESULTS: Incisional pain (26.9%), headache (11.6%), and drowsiness (11.5%) were the most frequently reported symptoms. Dizziness was reported by 9.7% and nausea/vomiting by 7.1%. Approximately 50% of patients undergoing laparoscopy, orthopaedic and general surgery reported 24-hr postoperative incisional pain. The incidence of 24-hr postoperative nausea/vomiting was highest after general 17.4%, orthopaedic, 11.2%, and laparoscopic surgery, 9.4%. Drowsiness was highest after laparoscopy 36.1%, followed by general surgery, 21.4%. Dizziness was most frequent after laparoscopy, 24.1%, followed by general surgery, 16.1%. After laparoscopy, postoperative drowsiness or dizziness was related to anaesthesia duration. After general surgery, postoperative dizziness or drowsiness were related to age; the younger the patient, the more likely the symptoms. CONCLUSIONS: Postoperative pain, nausea/vomiting, drowsiness, dizziness, and headache were the more frequent postoperative symptoms 24 hr after ambulatory surgery and they were influenced by the type of surgical procedure. In addition, the type of surgery and the 24-hr postoperative symptoms determined the degree of return to daily living function.

Adult↗
PubMed1996

Toward the construction of integrated physical and genetic maps of the mouse genome using interspersed repetitive sequence PCR (IRS-PCR) genomics.

Using two recently developed techniques, IRS-PCR YAC walking and IRS-PCR genotyping, a framework-integrated physical and genetic map of the mouse genome was constructed. The map consists of 821 contigs, containing 7746 YAC clones originating from three different YAC libraries. Three hundred eighty of the contigs have been anchored to the genetic map. Approximately 16% of the physical length of the mouse genome is estimated to be represented.

Animals↗
PubMed1996

Investigation on inhibition of biological effects of endothelin.

The effects of a series of substances on the biological function of endothelin (ET) are reported. The substances used are: synthetic inhibitors of endothelium derived relaxing factors (EDRFs), inhibitor of big-endothelin converting enzyme phosphoramidon, antiserum of endothelin, antagonists of endothelin A receptor BQ123 and JKC301, and two Chinese anti-snake venom herb medicines Lobelia radicans Thumb and Taris polyphylla Smith var. chinensis (Franch) Hara. The results showed that inhibiting the production of nitric oxide (NO) could stimulate ET release from vascular endothelium, elevate plasma ET and increase blood pressure. These changes could be reversed by L-arginine (L-Arg), the substrate of nitric oxide synthase (NOS). The amount of ET released by arterial endothelium could be increased or inhibited by inhibiting or stimulating the synthesis of prostacyclin (PGI2). The plasma ET level and blood pressure in both SHR and WKY rats could be decreased by giving phosphoramidon (PhR). The above results indicate that the biological effects of ET could be antagonized by inhibiting the synthesis or release of ET, decreasing the level of plasma ET, blocking the binding of ET with its receptor and using some Chinese anti-snake venom herb medicines.

Animals↗
PubMed1996

Role of intracardiac angiotensin II in cardiac dysfunction of rat during septic shock.

OBJECTIVE: To observe changes in the content of myocardial angiotensin II and its role in cardiac dysfunction of rat during septic shock. METHODS: Septic shock model was produced by cecal ligation and puncture (CLP) operation on rats. Experimental rats were given captopril 15 mg.kg-1/d per os for 3 days before CLP operation. Mean blood pressure and left ventricular pressure were recorded. Myocardial angiotensin converting enzyme (ACE) activities were determined by a fluorometric assay and myocardial angiotensin II content was measured by radioimmunoassay. Highly purified membrane of sarcoplasmic reticulum (SR) was prepared from rat hearts. Assays were made of ATP-dependent Ca2+ uptake by cardiac SR and (3H) ryanodine binding to SR. RESULTS: Myocardial angiotensin II content increased by 51.5% (P < 0.01) at the 18th hour post CLP, meanwhile there was a decrease in left ventricular +/- dp/dtmax value and the impairment in Ca2+ uptake and (3H) ryanodine binding to cardiac SR. Preliminary administration of captopril reduced myocardial ACE activity and angiotensin II content, but increased left ventricular +/- dp/dtmax value. In comparison to shock group, the initial rate and the capacity of SR Ca2+ uptake were increased by 120% (P < 0.01) and 33.9% (P < 0.05), the Bmax value of (3H) ryanodine binding to SR was also elevated, while the Kd value remained unchanged. CONCLUSIONS: The elevated intracardiac angiotensin II, resulting from the activation of myocardial ACE during sepsis, probably serves as one of the important mediators participating in the pathogenesis of heart failure: the effects of angiotensin II may be associated with the disturbance of Ca2+ transport function of cardiac SR.

Angiotensin II↗
PubMed1996

[(Sp)-8-chloroadenosine 3',5'-cyclophosphate induced differentiation on human leukemia HL-60 cells].

(Sp)-octyl 8-chloroadenosine 3',5'-cyclophosphate(OCC), a newly synthesized cAMP analog, strongly induced growth inhibition and differentiation in human leukemia HL-60 cells. The effects were dose- and time-dependent and irreversible. In flow cytometry, OCC brought about a block at the G1 phase of HL-60 cell cycle. Determined by incorporation assay, OCC was shown to strongly inhibit DNA synthesis without affecting the synthesis of RNA and protein in HL-60 cells. OCC activated the protein kinase A(PKA) in the cytosol of HL-60 cells and inhibited its binding to cAMP. The activities of PKA in the cytosol of HL-60 cells treated with OCC were more significantly increased than those in control cells. It can be concluded that OCC binds itself to PKA in competition with cAMP and, as a result, activates PKA.

8-Bromo Cyclic Adenosine Monophosphate↗
PubMed1995

Antibiotic C3368-A, a fungus-derived nucleoside transport inhibitor, potentiates the activity of antitumor drugs.

Antibiotic C3368-A (CA) is produced by a fungus strain from a soil sample collected in Antarctica. CA markedly inhibited radiolabeled thymidine and uridine transport in mouse Ehrlich carcinoma cells, its 50% inhibitory concentration (IC50) being 4.6 and 7.7 microM, respectively. In clonogenic assay, CA displayed a synergistic effect with methotrexate (MTX), mitomycin C (MMC), 5-fluorouracil (5FU), and Adriamycin (ADR) against human oral epidermoid carcinoma KB cells. CA also markedly enhanced the inhibitory effect of 5FU and ADR on the proliferation of human hepatoma BEL-7402 cells as determined by the p-nitrophenyl-N-acetyl-beta-D-glucosaminide (NAG) enzyme-reaction assay. 5FU or ADR cytotoxicity was not augmented by CA in human fetal lung 2BS cells. In vivo, CA significantly potentiated the inhibitory effect of MMC against colon carcinoma 26 in mice. No significant augmentation of toxicity by the combination was found in treated mice. The results suggest that CA, the newly found nucleoside-transport inhibitor, may be useful in potentiation of the effect of antitumor drugs.

Animals↗
PubMed1995

A new cytotoxic dihydroxy sterol from the soft coral Alcyonium patagonicum.

A new sterol, 24-methylenecholest-4-ene-3 beta,6 beta-diol [1] was isolated from the soft coral Alcyonium patagonicum collected from the South China Sea. Its structure was determined by spectral analysis. Compound 1 was cytotoxic against the P-388 cell line with an IC50 value of 1 microgram/ml.

Animals↗
PubMed1995

[An epidemiological study on reproductive effects in female workers exposed to acrylonitrile].

Four hundred and seventy-seven female workers exposed to acrylonitrile and 527 controls were studied by retrospective cohort method. Results showed incidence rates of pernicious vomiting and anemia, preterm delivery, and birth defects in exposed women were obviously higher than those in controls, with statistical significance. Logistic regression analysis revealed exposure to acrylonitrile in pregnant workers caused increasing risk of preterm delivery and birth defects. In addition, illness, medicine taking and X-ray irradiation during pregnancy related to the increase of incidence of preterm delivery in exposed women.

Acrylonitrile↗
PubMed1995

[Chest radiograph in assessing the results of treatment with 6-month short-course chemotherapy of pulmonary tuberculosis].

Chest radiograph was evaluated in 287 cases of smear-positive pulmonary tuberculosis treated with 6-month short course chemotheapy. After 6-month chemotherapy was completed, the results of chest rediography were in a similar trend comparing to that of sputum examination in 94.0% cases. During 18 months follow-up in 107 (37.9%) of 282 cases, the remained shadows in chest radiography continued to resolve and in 14 (58%) of 24 cases, remained in chest radiograph were closed. It is concluded that sputum examination in assessing the results of 6 months short-course chemotherapy simple, reliable and cheaper than chest radiography.

Adolescent↗
PubMed1995

[Effect of ginger-processing on beta-sitosterol and total alkaloid contents in Rhizoma Pinelliae].

The contents of beta-sitosterol of five processed products of Rhizoma Pinelliae we were determined by TLC. The result showed that the lowest content of beta-sitosterol was found in Rhizoma Pinelliae boiled with ginger juice (0.0180%), and the highest in raw Phizoma Panelliae (0.0572%). The contents of total alkaloid of five processed products of Phizoma Pinelliae were also determined by gravimetry and spectrophotometry with acid dye. The result showed that the highest content of total alkaloid was obtained in Rhizoma Pinelliae boiled with ginger juice and alum, and the lowest in Rhizoma Pinelliae boiled with ginger juice.

Alkaloids↗