Vascular supply of the pancreatic segment and its suitability for transplantation.
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Biomedical subjects
Publications and source records attributed to J Stingl.
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An original simple method was successfully worked out when following systematically the ultrastructure of particular microcirculatory areas of the m. spinotrapezius in the white rat. The main principle of this method consists in a precise orientation of the selected parts of the terminal vascular bed, beginning from their vital observation, continuing through their fixation and dehydration and ending with their embedding into a thin layer of Epon in order to prepare blocks containing very small of the beforehand-chosen areas of the vessels studied. A concise survey of the results obtained is given with a description of the main structural elements specific for the wall of the arterioles, terminal and precapillary arterioles, capillaries, postcapillaries and larger venules. The method presented has proved to be practically useful and is recommended for further investigations concerned with the submicroscopical structure of muscular vessels.
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The submicroscopic structure of the precapillary arterioles with a diameter of 25-6 mum was followed in m. spinotrapezius of the rat. The walls of the precapillary arterioles of the diameter of 25-20 mum consist of the endothelium, one layer of smooth muscle cells, 3-4 bundles of nerve fibres and a one-layer coat of fibrocytes. Arterioles of the diameter of 20-10 mum have an incomplete fibrocytar coat. Arterioles of the diameter of 10-6 mum also have an incomplete, disseminated muscular layer. The side-branching of true capillaries was not detected. Hence the described arteriolar portion cannot be considered as a metarteriole, but as a regulation device of a segmentary type, regulating the blood flow through the corresponding part of the capillary network as a whole.
The development of changes appearing with the closure of ductus arteriosus was followed in mature rabbit foetuses with the help of light and electron microscopy. In the foetuses, in which there was no spontaneous respiration of atmospheric air before fixation the ductus remained opened. In smooth muscle cells of the media there were enlarged cisternae of the endoplasmic reticulum being partially decayed. The foetuses that respired for about one minute had the ductus contracted to various degree and the endothelial cells were expelled into the lumen. In the foetuses respiring for 10 minutes the ductus were more contracted. The endothelium was considerably waved and the agglomeration of smooth muscle cells appeared. In the endothelial and musclar cells the endoplasmic reticulum was richer, and in a lot of places considerably disturbed. The ductus of foetuses respiring for 15 minutes had the lumen completely closed. The smooth muscle cells had a significantly changed their internal structure. The possibility of the direct transformation of the endoplasmic reticulum of the smooth muscular cells into inclusion bodies during the ductus arteriosus closure is discussed.
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