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Biomedical subjects

J Steinmann

Publications and source records attributed to J Steinmann.

83 records · Page 5Linked to original sources
PubMed1982

[Elimination of bacteria and viruses by flocculation in treated sewage].

In laboratory experiments the elimination of bacteria and viruses in treated sewage by different flocculants varied between 47.9 and 99.5%. Ferric and aluminum chloride reduced both groups of microorganisms in a greater extent than polyelectrolytes, among those the cationic polymers were better suitable than nonionic and anionic types. With a combination of an anorganic flocculant and an polyelectrolyte no increased effect was seen according to the elimination of the colony number and the number of viruses.

Aluminum↗
PubMed1981

Detection of rotavirus in sewage.

FOR DETECTION OF ROTAVIRUS, DOMESTIC SEWAGE WAS CONCENTRATED BY TWO DIFFERENT METHODS: (i) adsorption to and elution from positively charged Seitz filters, followed by ultracentrifugation, and (ii) chemical precipitation. The concentrated fluids were tested by an enzyme-linked immunosorbent assay and electron microscopy. In 6 of 24 (25%) samples, rotavirus was detectable after the combined filtration and ultracentrifugation technique with both an enzyme-linked immunosorbent assay and electron microscopy. No positive results were obtained after chemical precipitation.

Journal Article↗
PubMed1981

[Rotavirus in acute gastroenteritis (author's transl)].

518 stool specimens from patients with acute gastroenteritis, presumed to suffer from infections with enteropathogenic E. coli, were screened for rotavirus. Rotaviruses were found in 23.3%. Specimens containing virus mainly belonged to hospitalised infants aged less than 6 months. In 4 cases enteropathogenic E. coli were found in addition to rotaviruses. The enzyme-linked immunosorbent assay was more sensitive than complement fixation for the detection of rotaviruses and should be used for the differential diagnosis of acute gastroenteritis.

Adult↗
PubMed1978

[About the importance of chemical flocculation of wastewater in regard to hygienical aspects (microbiological and virological examinations in a wastewater treatment plant) (author's transl)].

In a small wastewater treatment plant corresponding samples from the intake and outtake of the chemical flocculation were chemically, microbiologically and virologically investigated and compared. It was found that both, the plate count and the number of coliforms, decreased about 90% to 95%. The phosphate content went down about 88%. The BOD5 reduction was ca. 80%, the COD reduction ca. 50%. Poliomyelitisviruses were found regularly in the intake but never in the outtake. Specially in regard to the high plate count- and virusreduction the chemical flocculation seems to be remarkable from the hygienical point of view not only for limnic but also for coastal waters.

Escherichia coli↗
PubMed1978

[Detection and persistence of human-pathogenic viruses in chemical sludge (author's transl)].

Virological investigations, using poliovirus type I, were carried out on the detection of enterovirus and their persistence in the chemical sludge from a tertiary treatment process, which could be suitable for agricultural and horticultural applications.--A comparison of six eluents showed that tryptose phosphate broth yielded best results allowing approximately 30% overall recovery with the method described. With sludge of low virus content a concentration was possible with alginate filters and the phase separation method.--Examinations on the persistence of poliovirus show that at 5 degrees C and 10 degrees C the inactivation was slow and that virus was detectable over a 15 week period at 5 degrees C.

Enterovirus↗
PubMed1978

[Detection of rubella virus antibodies in single radial haemolysis-test (author's transl)].

Different kinds of erythrocytes (human, pigeon, chicken and sheep) and complement (guinea pig, rabbit and rat) were tested in rubella single radial hemolysis-test (SRHT, hemolysis-in-gel = HIG-test) in order to detect IgG and IgM antibodies. Experiments showed that sera which were used as complement, contained antibodies against different kinds of erythrocytes. If complement from rabbit and rat should be used, an adsorption will be necessary. For serological surveillance sheep and chicken erythrocytes in connection with guinea pig serum as complement were best suited. Positive results were also obtained with pigeon erythrocytes whereas human RBC gave negative reactions. A detection of IgM antibodies in the SRHT was not possible.

Animals↗
PubMed1977

[Serum-treatment in the rubella hi-test by menas of silicic acid (author's transl)].

The serum treatment for the removal of nonspecific inhibitors in the Rubella HI-test with silicic acid was investigated by means of isolated lipoproteins and after a micromethod by means of Sindbis-virus. Reduction of the three classes of lipoproteins after treatment with silicic acid was good. In the Sindbis HI-test only 2% of the sera showed low titers after treatment. The method proved to be a reliable tool as removal of unspecific inhibitory activity is concerned.

Female↗
PubMed1977

[Lipoproteins as serum inhibitors in rubella hemagglutination inhibition test and their elimination (author's transl)].

Lipoproteins of 20 human sera were isolated by flotation centrifugation and tested for inhibitory activity in rubella HI-test. Low density lipoproteins (LDL) showed the highest titers with an average of 1:238,0, whereas the others (VLDL, HDL) had lower titers (see tab. 2). The greatest reduction of all classes of lipoproteins was achieved with kaolin treatment. Heparin-manganous chloride and dextran sulfate-calcium chloride method mainly reduced the LDL (84.4 resp. 78.7%). In a comparative test sera treated with the heparin procedure showed higher antibody titers than those treated with dextran sulfate.

Adult↗