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Biomedical subjects

J Stanek

Publications and source records attributed to J Stanek.

At least 73 records · Page 4Linked to original sources
PubMed1982

Application of the AOAC multi-residue method to determination of synthetic pyrethroid residues in celery and animal products.

A procedure based on current multi-residue methodology is described for the determination of synthetic pyrethroid residues in vegetable and animal tissues. Permethrin, cypermethrin, and fenvalerate are extracted from celery, egg yolk, beef muscle, and milk with acetonitrile, partitioned into hexane, and cleaned up on Florisil for quantitation by electron capture gas chromatography. Recoveries of the 3 pyrethroids averaged 94-103% from celery and 82-97% from animal products. Minimum detectable levels of less than 5 ng/g are readily attainable. The method also allows for the simultaneous extraction and cleanup of the organochlorine hydrocarbon insecticides which have been routinely analyzed in the past.

Animals↗
PubMed1981

Efficacy and dissipation of permethrin for the control of the northern fowl mite in hens.

Laying hens were treated with permethrin at the rate of 20 mg per bird to determine efficacy against northern fowl mites Ornithonyssus sylviarum (Canestrini and Fanzago) and the dissipation of residues over a 42-day period. Permethrin provided greater than 99% control of mites for at least 42 days posttreatment as compared to malathion which did not substantially reduce mite infestations. Carbaryl and coumaphos treatment resulted in limited reductions with mite populations recovering by two to three weeks posttreatment. Maximum residues of permethrin in breast muscle, body fat and the uropidium occurred on day 1 posttreatment and were still present in fatty tissue in detectable quantities on day 42. Residues in egg yolk appeared on day 3 posttreatment and peaked on day 7 with trace quantities still present on day 21. Permethrin was not found in egg white. Low residues were found in the liver and gizzard from day 1 through to day 7.

Animals↗
PubMed1977

Squamous metaplastic cells in cervical canal aspiration smears in women with ovarian function disorders.

Clinical findings and cytological data of 100 non-pregnant in-patients with squamous metaplastic cells in their cervical canal aspiration smears were compared to 100 randomly selected women without metaplastic cells, both groups having been diagnosed as ovarian disturbances. In women with squamous metaplastic cells more frequent cervical canal erosions, abnormal results of water-salt test and diagnosis of hypothalamic postpregnancy syndrome were stated. The findings together with the drift to the right of the Maturation Index, higher the Maturation Value and Eosinophilic Index and lower the Cytolytic Index, may suggest that the disturbances of the neurohormonal background are important in pathogenesis of the endocervical squamous metaplasia and that the latter may play a role in carcinogenesis.

Adenocarcinoma↗
PubMed1977

Cervical canal aspiration smears in hormonal cytodiagnosis.

On the basis of 200 women with functional ovarian disturbances analysed, it has been found that though cervical canal aspiration smears along cannot serve in hormonal diagnosis they can be valuable when estimated together with vaginal smears, particularly in the cases with the Intermediate Cell Complex. In oestrogen deficiency cytologic indices of CCAS are close to those of VS, cervical mucus is opaque, gray, homogenous and abundant in leukocytes and histiocytes; and glandular cells, both endometrial and endocervical, are hypothrophic. In oestrogen and androgen stimulation, high M.V., S.C.I., and E.I. and low N.I., C.I., F.I. and C., abundant, transparent, polychromatic and membranaceous cervical mucus scant in leukocytes and histiocytes; and typical oestrogenic appearance of the endometrial and endocervical cells are observed in CCAS, as compared to VS, where high C. and leukopenia are typical. In dried smears, arborization of the cervical mucus is usually seen in these cases. In luteal stimulation, M.V., S.C.I. K.I., and E.I. are slightly elevated, N.I., C.I., F.I., and C. are lower, cervical mucus is opaque, gray, and homogenous, abundant in leukocytes and scant in histiocytes, endometrial glandular cells are secretory, and endocervical cells are of luteal type in CCAS in comparison to VS. Some residual arborization phenomenon is frequenyc present in dried mucus. These preliminary results require further studies.

Androgens↗
PubMed1976

Comparative prognostic value of serum placental and tissue oxytocinase, alkaline phosphatase and its heat-stable fraction in pregnancy at neuroendocrinological risk.

Serum enzyme determinations are now well-established diagnostic tools in so-called "placental insufficiency". A good predictability of oxytocinases (P-CAP-placental oxytocinase and T-CAP-tissue oxytocinase) and a doubtful one of those of phosphatases (AP-alkaline phosphatase, HSAP-heat stable alkaline phosphatase) has been shown in high-risk pregnancies. The purpose of this study was to determine the prognostic value of the above cited enzymes in the so-called "pregnancy at neuroendocrinological risk", i.e. pregnancy in women with a prepregnancy history of hormonal disorders. It was shown that the outcome and results of such pregnancies are poorer that those of normal pregnancies. The series studied comprised 364 pregnant patients with pregnancy at neuroendocrinological risk that were being monitored by means of serum assays of the four enzymes. An attempt was made to assess each of these enzyme activities both in single (at least one value below 2.5 percentile calculated for healthy subjects) and serial determinations (two consecutive results decreasing or remaining at the same level). Normal and abnormal enzyme results were compared with normal and abnormal conditions of the newborn. The results presented showed that P-CAP (Tab. I) and T-CAP (Tab. II) levels were useful in prenatal diagnosis of fetal impairment in heneral, in addition to perinatal mortality and low values of the APGAR score. Neither the single nor serial assays of AT (Tab. III) and HSAP (Tab. IV) were valuable in predicting birth of an impaired neonate. Sensitivity of the test, i.e. percentage of women with abnormal enzyme assays among those patients who gave birth to impaired neonates, and specificity of the test, i.e. the percentage of women delivered of impaired neonates among all women with abnormal enzyme assays, of the four enzymes were compared. Sensitvity and specificity of P-CAP and T-CAP were higher than those for AP and HSAP. Moreover, sensitivity for all four enzymes was higher in serial assays, and specificity was higher in single assays. The results of the present analysis demonstrated the prognostic value of oxytocinase assays also in the pregnancy at neuroendocrinological risk. Assays of P-CAP and T-CAP were of equal significance, notwithstanding reports of a greater usefulness of P-CAP. Assays of CAP were helpful particularly in the conditions on which neuroendocrinological gestosis exerts a direct influence, i.e. in low Apgar score and perinatal mortality. On the other hand, serum alkaline phosphatases proved useless in endocrine pathology of pregnancy and HSAP was not superior to AP. About one half of future mothers of impaired neonates had enzyme results outside the range of the assays under consideration. This could be explained by the fact that these enzymes activities reflect placental function and are not directly related to fetal metabolism. Because of that they should be supplemented by other diagnostic methods being used in a clinic of high-risk pregnancy.

Alkaline Phosphatase↗