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Biomedical subjects

J Stamm

Publications and source records attributed to J Stamm.

At least 19 recordsLinked to original sources
PubMed2006

[Integrated Health Care in cardiology: have all players used their opportunities?].

From the view of one of the largest public health insurance companies, in this paper it is outlined that Integrated Health Care provides opportunities to influence the largely regional structures of health care supply. This insurance company has specialized in contracts for Integrated Health Care in cardiology in order to achieve sustainable solutions for all providers of invasive and interventional cardiology that are economically reasonable. But first of all, only evidence-based medical procedures and interventions based on clinical pathways are eligible for the contracts of this company. In outlining this, it becomes evident that by far not all German public health insurance companies have yet used their opportunity.

Cardiology↗
PubMed2005

[Implementing integrated health care--current state and progress].

The legal framework for Integrated Health Care since 2004 offers many opportunities for health professionals and insurers in the competition for "high quality care". Germany's largest statutory health insurance fund, BARMER, has already developed around 100 Integrated Health Care Projects for a wide range of conditions. One important issue is the relationship to disease management programs. Our program "integrated cardiological care" combines both DMP and integrated health care and thus already shows good results with respect to patient acceptance as well as savings trough the changing patterns in interventional cardiology.

Cardiology↗
PubMed1998

Direct spectroscopic detection of molecular dynamics and interactions of the calcium pump and phospholamban.

In order to test molecular models of cardiac calcium transport regulation, we have used spectroscopy to probe the structures, dynamics, and interactions of the Ca pump (Ca-ATPase) and phospholamban (PLB) in cardiac sarcoplasmic reticulum (SR) and in reconstituted membranes. Electron paramagnetic resonance (EPR) and phosphorescence of probes bound to the Ca pump show that the activity of the pump is quite sensitive to its oligomeric interactions. In cardiac SR, PLB aggregates and inhibits the pump, and both effects are reversed by PLB phosphorylation. Previous analyses of PLB's oligomeric state were only in detergent solutions, so we used EPR and fluorescence to determine the oligomeric structure of PLB in its native state in lipid bilayers. Wild-type PLB is primarily oligomeric in the membrane, while the mutant L37A-PLB is monomeric. For both proteins, phosphorylation shifts the dynamic monomer-oligomer equilibrium toward oligomers, and induces a similar structural change, as indicated by tyrosine fluorescence; yet L37A-PLB is more effective than wild-type PLB in inhibiting and aggregating the pump. Fluorescence energy transfer shows that the Ca pump increases the fraction of monomeric PLB, indicating that the pump preferentially binds monomeric PLB. These results support a reciprocal aggregation model for Ca pump regulation, in which the Ca pump is aggregated and inhibited by association with PLB monomers, and phosphorylation of PLB reverses these effects while decreasing the concentration of PLB monomers. To investigate the structure of the PLB pentamer in more detail, we measured the reactivities of cysteine residues in the transmembrane domain of PLB, and recorded EPR spectra of spin labels attached to these sites. These results support an atomic structural model, based on molecular dynamics simulations and mutagenesis studies, in which the PLB pentamer is stabilized by a leucine-isoleucine zipper within the transmembrane domain.

Animals↗
PubMed1994

Task-induced differential cortical activation pattern.

Measures of task-dependent cortical activation were assessed by bilateral EEG recordings from frontal, temporal, parietal and occipital areas. Two pictorial tests, the Raven Advanced Progressive Matrices and the Space Relations Test were used for verbal and spatial conditions, respectively. Recordings were obtained for 20 trials of each task from 22 right-handed adult males and 16 s. trial epochs were subjected to Fast Fourier analyses. Averaged intensity values for the alpha band were compared between verbal and spatial tasks for all subjects and between subgroups of verbalizers and visualizers, allotted on the basis of subject's performance index, derived from response speed and accuracy on the two tasks. The results were as follows: (1) The most pronounced EEG discriminators between the two performance subgroups are the left and right parietal and the right frontal area; (2) The left parietal zone provides the most pronounced discrimination between two groups; (3) There were significant interactions between the left parietal and the right frontal region; (4) The two parietal areas show characteristic frequency shifts in opposite directions for the task conditions. The findings imply complex interplay among the two parietal and right frontal areas, associated with sequential and holistic strategies. The results urge researchers to take into consideration subjectively preferred cognitive strategy, which along with objective task demands influences the process of problem solving and accompanying physiological changes.

Adult↗
PubMed1994

Effect of peritoneal fluid supplemented with exogenous progesterone on sperm motility in vitro.

Since progesterone has been claimed to induce acrosomal reaction and hyperactivated motility of human spermatozoa, the present study was undertaken to determine if its presence at concentrations similar to those of peri-ovulatory follicular fluid could influence the effect of peritoneal fluid on sperm motility in vitro. To this end, 11 sperm samples were incubated at 37 degrees C with five peritoneal fluids with/without exogenous progesterone, and sperm motility was assessed using a computer-assisted analyser at time (t) = 0, 2.5, 5 and 24 h. Overall there was no observable constant trend for enhancement or inhibition of sperm motility. Progesterone generally induced a negative effect on those sperm samples with high velocities in the native peritoneal fluids and a positive effect on those sperm samples demonstrating low motility in the native peritoneal fluids. The incorporation of progesterone into the incubation medium seemed to result in a 'tuning' of sperm velocity to around 30-50 micron/s. However, a given sperm sample reacted differently when incubated with various peritoneal fluids and, reciprocally, different semen samples incubated with the same peritoneal fluid showed very variable motility patterns. The greater variability of the effects exerted by progesterone on sperm motility could arise from the fact that each sperm sample may contain subpopulations of gametes with different sensitivity to progesterone.

Adult↗
PubMed1992

Effect of volumetric mixtures of peritoneal and follicular fluid from the same woman on sperm motility and acrosomal reactivity in vitro.

OBJECTIVE: To study the effect of peritoneal (PF) and follicular fluids (FF from the same woman) as well as of given volumetric combinations thereof on sperm motility and acrosomal reactivity. DESIGN: Prospective. Peritoneal fluid and FF were incubated separately or in given volumetric combinations (PF/FF = 100/0, 75/25, 50/50, 25/75, 0/100; vol/vol) with swim-up sperm suspensions. SETTING: In vitro fertilization and general infertility clinic and laboratories. PATIENTS, PARTICIPANTS: Women participants of the gamete intrafallopian transfer program (motility study, n = 20; acrosomal reaction study, n = 14). Sperm donors of the artificial insemination program and men with given sperm parameters. INTERVENTIONS: Hormonal stimulation. Laparoscopy. MAIN OUTCOME MEASURES: Progressive velocity and percentage of motile gametes measured with multiple-exposure photography. Acrosomal reactivity measured by an immunofluorescent technique. RESULTS: Follicular fluid always influenced progressive motility and also sustained the number of motile gametes, as function of time, better than PF or the PF/FF mixtures (P less than 0.05). The acrosomal reactivity of sperm incubated in the various PF/FF combinations was low; after 5 hours only the FF-sperm suspensions showed a significant enhancement of acrosomally reacted gametes. CONCLUSION: At ovulation, FF transmit positive (motility- and acrosomal reactivity-enhancing) signals to sperm, whereas PF may transmit positive, neutral, or negative signals (noise signals). The volumetric combination of FF and PF in the tubal environment, which may differ from cycle to cycle and from woman to woman, could therefore result in synergic (or antagonistic) effects on the sperm fertility potential.

Acrosome↗
PubMed1990

Excerpts from presentation on epidemiologic data and changing demographics.

Dental surveys provide epidemiologic and sociodemographic data useful for program planning, implementation, and evaluation. Dental public health decisions in North Carolina have been based on statewide oral health surveys. Results from the 1986-87 North Carolina School Oral Health Survey and a comparison of these data to previous survey results and to recent national estimates of oral disease provide a basis for the discussions and deliberations during the symposium.

Adolescent↗
PubMed1987

The role of adjunctive Rodogyl therapy in the treatment of advanced periodontal disease. A longitudinal clinical and microbiologic study.

Several studies have indicated that the combination of metronidazole and spiramycin is synergistic against anaerobic bacteria and may be effective against oral infections. The present study sought to determine the efficacy and safety of a commercial preparation of these two antibiotics (Rodogyl) when used adjunctively in the treatment of advanced periodontal disease. In a double-blind parallel randomized trial, 56 patients (mean age = 44 years) with advanced periodontitis (50 of whom completed the study) were assigned to either the Rodogyl or placebo group. Both groups were thoroughly scaled and root planned for approximately 6 hours, with one group receiving Rodogyl for 2 weeks and the other a placebo. No other therapy was received during the study period. Two sites in each patient with probing depths of at least 7 mm were selected for study. Plaque level (P1I), gingival inflammation (GI), probing depth (PD), and attachment level (AL) were measured at baseline, 14 days, 1 month, and then at monthly intervals up to 6 months. Subgingival bacteria were monitored with dark-field microscopy. The development of resistant bacteria, as well as side effects to the medications, was also monitored. The Rodogyl group exhibited a greater gain in AL (0.67 mm) from the 2-month interval until the end of the study. Although this difference was statistically significant (P less than 0.05), it was not necessarily of biologic significance. There was a significantly greater decline in the proportion of spirochetes in the Rodogyl group at the 14-day interval, and this difference remained significant (P less than 0.05) at all study intervals. No difference in the proportion of motile organisms was observed.(ABSTRACT TRUNCATED AT 250 WORDS)

Adult↗
PubMed1987

[Cerclage by the abdominal route].

The authors, having carried out two cerclage operations through the abdominal route for cervico-isthmic incompetence, review the literature and assess the advantages and disadvantages of this method. This surgical technique makes it possible to put the stitch exactly at the level that is needed. It can be carried out when the cervix is very short, effaced or totally distorted. The results that have been obtained in the larger series are striking: the percentage of infants that survived before the operation was between 10.4% and 24% and after the operation between 70% and 95%. The complications described in the literature have been rare: haemorrhage from damage to the veins at the time of the operation: fetal death in the days after the stitch had been placed badly so that the uterine vessels were occluded. As far as we know, there has never been a case of infection.

Abdomen↗
PubMed1983

[The peritoneal sperm migration test (PSM-test) following fractional artificial insemination during laparoscopic fertility testing].

The peritoneal sperm migration test following fractional artificial insemination during diagnostic laparoscopy was carried out in the context of fertility investigation in 78 women age 25 to age 40. 35 cases had a positive PSM test, 43 had a negative PSM test. The results were correlated to the phase of menstrual cycle, the semen analysis and the tubal patency test. The test showed the best results when it was done in the preovulatory phase in conjunction with a normal semen analysis and a normal tubal patency test. In 2 cases with severe oligospermia and in two cases with bilateral tubal occlusion the test was still positive. The test is of clinical importance and can be done without increased risk during any laparoscopy for infertility. Valuable conclusion about the migration of sperms through the uterus and the tubes in the individual couple can be obtained. This is of prognostic importance. The test shows in addition to mechanical tubal patency by chromopertubation the functional or physiologic tubal permeability. Negative PSM tests are relatively frequent and are caused by tubal, uterine or sperm factors.

Adult↗
PubMed1982

Spontaneous cortical slow-potential shifts and choice reaction time performance.

EEGs from Fz, Cz and Pz locations (reference--right mastoid) were obtained from 36 adults. For experimental trials the Fz slow potentials (SP) were determined for a pretask interval of: a 3 sec baseline, followed by a 2.5 sec SP shift, in either negative (3.5 microV) or positive (1.5 microV) direction, and maintenance at this level for another 2.5 sec. When this criterion was met the stimuli for a choice RT task were presented (potential-related event, PRE), which required a button-press response with the right or left hand for differing stimulus configurations. During subsequent control sessions subjects were tested without pretask criterion SP shifts, with yoked pretask intervals from the experimental sessions. For the experimental conditions the mean RTs were significantly faster and less variable with the negative than with the positive SP shift condition. For the control conditions mean RTs were somewhat faster for the positive than for the negative criterion, which might be attributed to the more variable pretask intervals during the latter polarity condition. For confirmation of the cortical localization of this effect, the experimental session was replicated with corresponding negative and positive SP shift criteria from Pz. No significant differences among means and standard deviations of RTs were found. SP shifts from criterion electrodes (Fz or Pz) were significantly greater in mean amplitude than the concomitant SP shifts from the other electrodes. The present findings demonstrate the feasibility of the PRE methodology for investigations of human brain functions.

Adult↗
PubMedDate not supplied

Growth hormone does not attenuate the inhibitory effects of sepsis on wound healing.

Chronic abdominal sepsis is associated with impaired tissue repair. Treatment of burn patients with growth hormone results in improved healing of skin graft donor sites. The goal of this study was to determine whether administration of growth hormone could attenuate the inhibitory effects of sepsis on cutaneous wound healing. Four groups of male Sprague Dawley rats were studied: control, control + growth hormone, sepsis, and sepsis + growth hormone. Sepsis was caused by implantation of a bacterial focus in the peritoneal cavity. Control animals underwent sham laparotomy, and polyvinyl alcohol sponge implants were placed in subdermal pockets in all animals. Saline or growth hormone (400 microg) was injected subcutaneously every 12 hours. On day 5, the incisional wounds and polyvinyl alcohol sponge implants were harvested. The breaking strength of abdominal incisions was measured. Granulation tissue penetration and quality were determined by scoring polyvinyl alcohol sponge implant histology from 1 to 4 in a blinded fashion. Collagen deposition in polyvinyl alcohol sponge implants was quantitated by hydroxyproline assay. Septic mortality was not altered by growth hormone administration. Septic animals showed a reduction in food consumption for 2 days after surgery (p < 0.05 vs. controls), which was not affected by growth hormone administration. The breaking strength of incisional wounds and hydroxyproline content of polyvinyl alcohol sponge implants was reduced in septic rats (p < 0.001 vs. controls) but administration of growth hormone for 5 days did not improve breaking strength or collagen deposition in either group. We conclude that the administration of growth hormone for 5 days did not improve collagen deposition or breaking strength in cutaneous wounds from control or septic animals. The results suggest that growth hormone treatment is unlikely to improve tissue repair in sepsis-induced catabolic illness.

Animals↗