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Biomedical subjects

J Stam

Publications and source records attributed to J Stam.

99 records · Page 6Linked to original sources
PubMed1978

Concentrations of ampicillin in pleural fluid and serum after single and repetitive doses of bacampicillin.

In patients with pleural effusions due to malignant disease, estimations of ampicillin were made on simultaneously obtained samples of blood and pleural fluid: (1) from patients (n=11) after the administration of a single dose of 400 mg of bacampicillin, and (2) from patients undergoing treatment with 800 mg of bacampicillin twice daily (a) during the 12 hours after the first dose (n=10) and (b) during the 7 hours after the third dose (n=9). Bacampicillin gave concentrations in the pleural fluid well above the MIC of common pathogens including H. influenzae. Mean peak levels were 1.2 microgram/ml after the 400 mg dose and 3.8 microgram/ml after the first and third doses of 800 mg. The peak appeared later in the pleural fluid in comparison with serum peak levels. The elimination half life was considerably longer in the pleural fluid.

Ampicillin↗
PubMed1977

Lymphoblast transformation studies in sarcoidosis.

Tissue culture studies of lymphnode lymphocytes, obtained from Sarcoidosis patients revealed lymphoblast transformation (morphologically observed) after incubation with Kveim antigen. Simultaneously we could obtain peripherical blood-lymphocytes from some patients. Lymphocytes from both compartments showed a positive correlated blasttransformation. Spontaneous blasttransformation was observed in cultures supplemented with fetal calf serum, however, this phenomenon was not observed in cultures with human AB serum. The results, together with the discrepance of spontaneous lymphoblast transformation will be discussed.

Animals↗
PubMedDate not supplied

Low levels of specific T cell activation marker CD27 accompanied by elevated levels of markers for non-specific immune activation in the cerebrospinal fluid of patients with AIDS dementia complex.

Concentrations of soluble receptors for tumor necrosis factor (sTNFR-p55 and sTNFR-p75) and soluble T cell antigens CD25 and CD27 (sCD25 and sCD27) were measured in paired serum/cerebrospinal fluid (CSF) samples of 15 patients with AIDS dementia complex (ADC) and 15 HIV-infected control subjects (11 with other central nervous system (CNS) infections and four without CNS infection). In this study levels of sTNFR-p55, sTNFR-p75 and sCD25 were elevated in the CSF of ADC patients and of the 11 patients with other CNS infections, whereas CSF-levels of the specific T cell marker sCD27 were lower in patients with ADC as compared to the control subjects with and without other CNS infections. This pattern suggests a relative failure of eliciting a T cell-mediated immune response intrathecally in patients with ADC.

AIDS Dementia Complex↗
PubMedDate not supplied

Simultaneous enumeration of T-cell subsets and macrophages in bronchoalveolar lavage fluids by immunoenzyme double staining. Comparison with conventional immunofluorescence.

Bronchoalveolar lavage is a common research and clinical tool for the retrieval of cells from the lower respiratory tract. In addition to conventional morphologic study of these cells, the subtyping of T lymphocytes is often important for reaching a diagnosis of a disease or assessing its activity; subtyping is usually done by a standard immunofluorescence assay on cell suspensions requiring about 5 X 10(5) cells. Since the number of leukocytes in the lavage fluid from many patients is too small to obtain reliable information by this assay, a double immunoenzyme staining of T-lymphocyte subtypes on Cytospin preparations was utilized. This method, which requires a small number of cells, was compared with the standard immunofluorescence assay for the identification and quantitation of lymphocyte subtypes in the lavage fluids of patients with different disorders. Although the immunoenzyme double staining assay is somewhat more laborious, it provides important advantages: (1) simultaneous observation of two lymphocyte subsets and macrophages on the same slide; (2) a considerably smaller number of cells (2 X 10(4) instead of 5 X 10(5] is necessary; (3) the availability of permanent preparations; (4) the possibility of storing the Cytospin slides before staining; and (5) conventional light microscopy can be used. Since the reliability of both techniques appeared to be the same, the double staining assay for routine usage with bronchoalveolar lavage fluids appears to be preferable.

Bronchoalveolar Lavage Fluid↗