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Biomedical subjects

J Stadler

Publications and source records attributed to J Stadler.

At least 163 records · Page 9Linked to original sources
PubMed1972

Temperature dependence of sex-factor maintenance in Escherichia coli K-12.

Wild-type sex factors of Escherichia coli K-12 can be cured from host cells by growth at temperatures above 42 C. This phenomenon is obscured by reinfection of cured cells if the culture is allowed to reach cell densities above 10(5)/ml. The rate of episome loss is dependent on the genetic (chromosomal) background of the host.

DNA, Bacterial↗
PubMedDate not supplied

Interlaboratory evaluation of embryotoxicity in the postimplantation rat embryo culture.

The embryotoxicity of eight xenobiotic compounds in rat postimplantation whole embryo culture was blindly tested in four laboratories according to a standard protocol. The results show that the four nonteratogens amaranth, penicillin, isoniazid, and saccharin did not affect embryogenesis apart from general toxicity at very high concentrations in culture for amaranth and isoniazid. There was good concordance of results across the laboratories. The four teratogens (retinoic acid, 6-aminonicotinamide, acetylsalicylic acid, and vincristine) induced a variety of specific embryotoxic effects, which were in most cases similar in all laboratories. These results indicate that the definition for specific embryotoxicity used, as well as the culture duration and embryonic age are crucial for concordant scoring. Other methodologic differences did not significantly influence scoring of embryotoxicity. Therefore, within the limits of the end points and embryonic stage represented in the method, embryo culture appears as a useful method for embryotoxicity screening, which can be reproducibly applied in different laboratories.

Amaranth Dye↗