Biomedical subjects
J Spindler
Publications and source records attributed to J Spindler.
Functional loss of the horizontal doll's eye reflex following unilateral vestibular lesions.
The doll's eye reflex represents the vestibulo-ocular reflex (VOR) elicited by high-acceleration head rotation. After complete unilateral vestibular lesions, the ipsilateral, horizontal doll's eye reflex is replaced by a series of "catch-up" saccades. These cause permanent symptoms of blurred vision and dizziness during ipsilateral turns. We compared normal controls and patients with complete surgical lesions or canal paresis of up to 9 years duration via electronystagmography (ENG) to determine the usefulness of the doll's eye test as a diagnostic test for complete vestibular lesions. This test was found to be more sensitive in diagnosis of such lesions than head-shaking nystagmus, rotatory directional preponderance, and spontaneous nystagmus. It is also useful to document VOR function in patients in whom caloric irrigation is contraindicated.
Evaluating the thirty minute interval in emergency cesarean sections.
BACKGROUND: This study was done to evaluate what percent of emergency cesarean sections are begun within the 30 minute interval between decision and incision time and to evaluate morbidity associated with this time interval. STUDY DESIGN: A retrospective patient-control study of records from 75 patients undergoing emergency cesarean sections and two different control groups was undertaken. RESULTS: Sixty-three percent of emergency cesarean sections were begun in less than 30 minutes. A significantly greater number of infants in the group that delivered in less than 30 minutes experienced five minute Apgar scores less than six. There was no significant differences in maternal morbidity associated with emergency cesarean sections. CONCLUSIONS: The 30 minute interval is obtainable in a large number of patients but did not have a beneficial effect on neonatal morbidity. There was no significant morbidity seen in the patients who underwent emergency cesarean section. Other measurements of emergency preparedness should be considered other than the 30 minute rule.
Negative relationships between responses from head-shake and caloric tests.
Explore the source record for details and available documents.
[High precision, reproducibility and practical application of modified alanine aminotransferase determination with the microtiter plate].
In order to improve the alanine aminotransferase (ALT) assay by using microtiter plates, a new 96-channel multiple syringe of high precision and incubation chambers with precise regulation of the temperature operated by computer were developed. In accordance with the recommendation of the German Society of Clinical Chemistry for the optimized standard method, the ALT assay was modified for the procedure at 25 degrees C. The modification was comparable with the best conventional methods which, however, are very time-consuming. The ALT assay has the advantages of the microtiter plate, e.g., secure sample identification, on-line documentation and reduction of costs and time for the analysis.
Seeing through the mask of cancer.
Explore the source record for details and available documents.
Variations of biphasic head shake response: physiology and clinical significance.
Explore the source record for details and available documents.
Neuroleptic-induced "anhedonia" in rats: pimozide blocks reward quality of food.
The dopamine receptor blocker pimozide attenuated lever-pressing and running for food reward in hungry rats. In each case the characteristic behavior of pimozide-treated rats was the same as that of undrugged rats when reward was simply withheld. Drug-induced performance difficulties were ruled out by the presence of periods of normal responding in drug-treated animals. Pimozide appears to selectively blunt the rewarding impact of food and other hedonic stimuli.
Major attenuation of food reward with performance-sparing doses of pimozide in the rat.
Explore the source record for details and available documents.
Orbital and conjunctival involvement in multiple myeloma. Report of a case.
Benjamin, Ivy, Taylor, Herbert, and Spindler, James: Orbital and conjuctival involvement in multiple myeloma. Report of a case. Am J Clin Pathol 63: 811-817, 1975. Orbital and conjunctival involvement in multiple myeloma are rare. In most reported cases of multiple myeloma with orbital involvement proptosis has been the initial manifestation that has led to diagnosis of the disease. Our case represents and example of terminal involvement of the orbit and conjunctiva, causing proptosis. The minimal conjunctival changes were the first indication of neoplastic proliferation of plasma cells in this location and, presumably, the orbit.
Phlebography in the management of pulmonary embolism.
Explore the source record for details and available documents.
Efficacy of low doses of heparin in prevention of deep-vein thrombosis after major surgery. A double-blind, randomised trial.
Explore the source record for details and available documents.
Antibiotics, postoperative infection, and deep-vein thrombosis.
Explore the source record for details and available documents.
Soleal veins and stasis during operation.
Explore the source record for details and available documents.
Suppression of alcohol induced nystagmus by Innovar. A clinical study.
Explore the source record for details and available documents.
Improved alanine aminotransferase assay using the microtiter plate.
New procedures and new devices have been developed to improve the alanine aminotransferase (ALT) assay by using microtiter plates with 96 wells. Sera for ALT determination and reagents are distributed with a computer-controlled multiple syringe for simultaneous filling of the 96 wells. The volumes simultaneously distributed by this new multiple syringe show very little variation. The coefficient of variation (CV) is 0.6 percent (n = 96). Repeated filling of the wells with this syringe results in good reproducibility of distributed volumes (CV = 0.5%, n = 10). The temperature of sera, reagents, and mixtures, even during photometry, is actively regulated (down or up) to 25 degrees C in special chambers. Thus, the temperature varies minimally +/- 0.05 degrees C. ALT values in the low normal range as well as the high pathologic range show little variation (CV less than 2.3%) in aliquots of the same sample simultaneously or subsequently examined by the new procedure and with new devices. There is good correlation between the improved method using microtiter plates and the conventional methods (r greater than 0.9900, ALT values from 1-154 U/L). False-low and false-high values are excluded by computerized evaluation of many single determinations during the kinetic reactions in each sample. The new method is precise as well as simple, saves time, and may become important for the determination of other serum constituents on the basis of kinetics.
Development of a batch-processed, passive latex agglutination test for cytomegalovirus.
BACKGROUND: The passive latex agglutination test is commonly used for the identification of cytomegalovirus IgG and IgM antibodies. This test is used because of its sensitivity, specificity, speed, and ease of performance, but it is unsuitable for large numbers of samples or for batch processing. STUDY DESIGN AND METHODS: To solve this problem, comparative studies to assess the cytomegalovirus passive latex agglutination test on microtiter plates were done with a new photometric particle agglutination method (PPAM) and an enzyme-linked immunosorbent assay as a control. RESULTS: A total of 3430 sera were tested using both the PPAM and enzyme-linked immunosorbent assay. A high degree (97.6%) of correspondence between the results of the tests was observed. The new PPAM was easier and faster to use (96 wells in 25 min). CONCLUSION: These results, as well as the possibility of adapting this method to a fully automated system, suggest that the PPAM could be an important contribution to the field of infection serology.