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Biomedical subjects

J Skrha

Publications and source records attributed to J Skrha.

At least 145 records · Page 8Linked to original sources
PubMed1980

Bone isoenzyme of serum alkaline phosphatase in diabetes mellitus.

Increased activity of bone isoenzyme of serum alkaline phosphatase was found in 52, 82 and 72% of the patients on dietary, oral agents, and insulin regimens, respectively. Significant positive correlations between the activity of bone isoenzyme and urinary hydroxyproline excretion in diabetes are similar to those found in osteoporosis. In stepwise regression analysis, negative correlations were found between the parameters of turnover of bone organic matrix and serum calcium levels; the influence of blood glucose levels in the expression of the biochemical parameters of bone metabolism was most pronounced in patients on oral agents. The results explain moderate hyperphosphatasia, known in diabetes, and support a metabolic etiology for the bone disease in diabetes mellitus.

Adolescent↗
PubMed1979

Amylase isoenzymes in mumps.

The amylase isoenzymes activities in serum and urine from 75 children treated for complications of mumps were evaluated. The clinical observations, especially in cases with suspected pancreatitis, were compared with the P- and S-type amylase activities. Increased total activity of serum amylase in 79% of patients was due to increased S-type amylase. However, in 39% of patients there was also a markedly increased P-type serum amylase. Amylase isoenzymes may offer a new insights into the relationship between the pancreas and the parotid glands in mumps.

Amylases↗
PubMed1978

Isoenzymes of aspartate aminotransferase in chronic liver diseases.

The total activity and activity of the cytoplasmic and mitochondrial isoenzyme of aspartate aminotransferase was examined in blood plasma of 56 patients with chronic liver diseases (chronic hepatitis in 27, liver cirrhosis in 23, secondary neoplastic effection of the liver in 6). All the patients with biochemically active forms of liver disease manifested increased the total as well as cytoplasmic enzyme activity, as compared with control group, 57% of the patients manifested simultaneously also increased activity of the mitochondrial isoenzyme. In 13% of the patients with stabilised forms of liver diseases manifested isolated increase of the mitochondrial isoenzyme activity. This might be of importance for the evaluation of the course of the disease. In patients with tumorous metastases in the liver a strikingly high share and activity of mitochondrial isoenzyme was shown.

Aspartate Aminotransferases↗
PubMedDate not supplied

Serum lipase, isoamylase and pancreatic function test (PFT) in juvenile-onset insulin-dependent diabetes mellitus.

Significantly decreased activity of pancreatic isoamylase in serum was found in a group of 51 juvenile-onset insulin-dependent diabetics as compared to healthy subjects (p less than 0.005). No significant changes were observed for urinary p-aminobenzoic acid excretion in 20 of the juvenile-onset diabetics in whom the NBT-PABA test was performed, even though 25% of the values were below the normal limit. A highly significant decrease of serum lipase activity was found in juvenile-onset diabetics as compared to controls (p less than 0.001). No significant correlation was found in juvenile-onset diabetics between serum pancreatic isoamylase and lipase or marker of chymotrypsin activity expressed as the amount of p-aminobenzoic acid excreted into urine. The NBT-PABA test appears to be of small importance in the evaluation of changes of the exocrine pancreas in insulin-dependent diabetes mellitus. However, simultaneous evaluation of serum pancreatic isoamylase and lipase activities justified the suspicion of pancreatic damage in 50% of the patients tested.

4-Aminobenzoic Acid↗
PubMedDate not supplied

Computer models of albumin and haemoglobin glycation.

UNLABELLED: Two first-order mathematical models were developed to mimic the glycation of haemoglobin (H) and albumin (A). The total concentrations of A and H were assumed to be constant. The responses of the two models to varying blood glucose level were compared. The parameters of the haemoglobin model were not numerically estimated, only an informal fit was performed using clinical and published data. Nonlinear regression analysis was used to estimate the parameters of the albumin model. The level of glycated A (GA) was derived from the measured fructosamine level. Three diabetics were monitored daily for the level of fructosamine and blood glucose profile over a period of 10, 16 and 21 days, respectively. CONCLUSIONS: (1) The difference between GA and glycated H (GH) resulting from different elimination rates is decreased by the stratification of erythrocyte population. (2) Both GA and GH seem to have higher elimination rates than their nonglycated equivalents.

Blood Glucose↗
PubMedDate not supplied

Comparison of laser-Doppler flowmetry with biochemical indicators of endothelial dysfunction related to early microangiopathy in Type 1 diabetic patients.

The aim of this study was to compare biochemical markers of endothelial activation with microcirculation measured by laser-Doppler flowmetry in Type 1 diabetic patients with or without microangiopathy. A total of 44 Type 1 diabetic patients were subdivided into those with (n=24) and without (n=20) microangiopathy according to ophthalmological findings and the presence or absence of microalbuminuria. The control group consisted of 25 healthy people of comparable age, sex, and body mass index. Postocclusive reactive hyperemia (PORH) and thermal hyperemia (TH, at 44 degrees C) were measured at the forearm. Serum N-acetyl-beta-glucosaminidase (NAG) activity, serum E-selectin, and ICAM-1 concentrations were used as biochemical markers of endothelial dysfunction. A significantly lower velocity of perfusion increase during postocclusive hyperemia (PORH(max) x t(1)(-1)) and during thermal hyperemia (TH(max) x t(2)(-1)) (P<.01) were accompanied by higher serum NAG activity (20.9+/-4.6 vs. 16.3+/-2.5 U l(-1), P<.01) in diabetic patients with microangiopathy as compared to healthy persons. An inverse relationship was found between PORH(max) x t(1)(-1) and NAG (r=-.33) results in diabetic patients. In addition, higher mean values of serum NAG activity, E-selectin, and ICAM-1 concentrations were associated with significantly lower values of microcirculation parameters (PORH(max) x t(2)(-1) and TH(max) x t(2)(-1)) in six patients without microangiopathy who had at least one of the above biochemical markers higher than mean+2 S.D. range. We suggest that serum NAG activity, E-selectin, and ICAM-1 concentrations may be used together with laser-Doppler flowmetry in Type 1 diabetic patients as early indicators of vascular changes in very early stage of diabetic microangiopathy.

Adult↗