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Biomedical subjects

J Sirot

Publications and source records attributed to J Sirot.

129 records · Page 8Linked to original sources
PubMedDate not supplied

[Changes in the activity of antibiotics by fatty acids in bone tissue].

The C14 to C18 fatty acid content of spongious bone was measured by gas-liquid chromatography. Palmitic acid (C16:0), oleic acid (C18:1) and linoleic acid (C18:2) represented 20 to 40% of the total free fatty acid concentration (30 mM/l). Linoleic acid was found to have the greatest bacteriostatic activity against Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa strains. The effect of linoleic acid-gentamicin combination was synergistic against S. aureus. In contrast, both gentamicin and colistin activities were highly antagonized by linoleic and oleic acids against E. coli and P. aeruginosa strains. These different effects of free fatty acids on antibiotic activity could be explained, in part, by a change in permeability of bacterial cells. The clinical implications of these results in bone infections are discussed.

Anti-Bacterial Agents↗
PubMedDate not supplied

[Rifampicin diffusion in non-infected human bone (author's transl)].

Rifampicin levels in serum, cortical bone and spongious bone were measured by a biological assay in 13 non-infected patients undergoing total replacement of the hip. Samples were collected after 24-72 hours rifampicin administration (daily oral dose: 600 mg). The mean level was 0.67 +/- 0.48 microgram/g in cortical bone and 3.35 +/- 0.72 microgram/g in spongious bone. Rifampicin diffusion was also assessed in normal bone from crista iliaca needle biopsy specimens in 7 patients treated for osteitis or septic arthritis. Bone and serum levels did not appear linearly correlated but, in any case, bone levels were much higher than MIC for Staphylococcus aureus which is usually involved in bone and joint infections.

Arthritis, Infectious↗