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Biomedical subjects

J Simpson

Publications and source records attributed to J Simpson.

At least 253 records · Page 14Linked to original sources
PubMed1988

Evaluation of a computerised test for the assessment of peripheral vascular disease.

This paper reports a study carried out in 200 patients to assess the effectiveness of a clinical vascular laboratory in the routine assessment of peripheral vascular disease (PVD) of the lower limb. Laboratory assessments involved a computer based hierarchical testing system incorporating pedal pressure indices, maximum walking distances and principal component analysis of the common femoral artery blood velocity waveform. The study fell into two parts. In the first, the laboratory assessments of 100 patients referred six years ago were compared retrospectively with their eventual clinical outcome. In this comparison, the laboratory provided a "diagnosis" which was 79% correct. In the second, a double blind prospective study was carried out in 100 patients to compare the vascular laboratory "diagnosis" with the diagnosis and prognosis of a skilled clinician, the outcome being compared one year after the initial assessment. In this study the laboratory proved to be correct in 78% of cases, the clinician in 70%. With a slightly modified computer protocol for the non vascular diagnosis, the computer would have been correct in 85% of cases. This test provides a quantitative and objective assessment of PVD which can assist in the patient's clinical diagnosis and management.

Diabetic Angiopathies↗
PubMed1988

Research note: recurrent headaches and stomachaches in preschool children.

Data are presented on headaches and stomachaches in a sample of 189 3-year-old children obtained as part of community based study. These data provide a partial replication of an earlier study on a separate sample of children [Zuckerman, B., Stevenson, J. and Bailey, V. (1987). Stomachaches and headaches in a community sample of preschool children. Pediatrics, 79, 677-682]. The results supported the previous findings of a link between family factors and recurrent stomachache, and a less strong association with headaches. The conclusions reinforce the notion that these two somatic symptoms should be considered separately in preschool children.

Child Development↗
PubMed1988

Routine and rapid enzyme linked immunosorbent assays for circulating anti-glomerular basement membrane antibodies.

Microtitre plate modifications of the original tube enzyme linked immunosorbent assay (ELISA) of Wheeler and Sussman were used for the routine and rapid assays of anti-glomerular basement membrane antibodies in human sera. In a prospective study of 238 sera from 200 patients, the routine assay (about 24 hours) detected circulating antibodies in seven sera from three patients with active anti-glomerular basement membrane disease. The remaining sera, from patients with a variety of other glomerulonephropathies, were negative by the assay. The rapid assays took less than four hours, and in a retrospective study, detected anti-glomerular basement membrane antibodies in a range of 15 positive sera, with a level of discrimination similar to that observed in the routine assay and with no false positive/negative results.

Antibodies↗
PubMed1988

The role of fine needle aspiration cytology (FNAC) in the diagnosis of breast masses in males.

The role of fine needle aspiration cytology (FNAC) in the management of male breast masses has been reviewed. Forty-four male patients have been investigated in this way with one carcinoma being diagnosed. The cytological characteristics of the patients in this series with gynaecomastia and carcinoma of the male breast have been described. Although clinically the distinction between male breast carcinoma and gynaecomastia is often difficult, FNAC is an important aid in making this distinction. FNAC is recommended for the evaluation of all breast masses in adult men.

Aged↗
PubMed1987

Trichothecene mycotoxins in aerosolized conidia of Stachybotrys atra.

Stachybotrys atra is the etiologic agent of stachybotryotoxicosis, and this fungus and its trichothecene mycotoxins were recently implicated in an outbreak of unexplained illness in homes. S. atra was grown on sterile rice, autoclaved, dried, and then aerosolized by acoustic vibration. The distribution of particles (mass and number) was monitored on an aerodynamic particle sizer interfaced with a computer. Dust was collected on preweighed glass-fiber filters and extracted with 90% aqueous methanol. Extracts were tested for the ability to inhibit protein synthesis in rat alveolar macrophages, the ability to inhibit the proliferation of mouse thymocytes, and the presence of specific trichothecene mycotoxins. Virtually all of the particles were less than 15 micron in aerodynamic diameter, and the mass median diameter was 5 micron. Thus, most of the particles were respirable. Microscopic analysis of the generated dust revealed that ca. 85% of the dust particles were conidia of S. atra, another 6% were hyphal fragments, and the remainder of the particles were unidentifiable. Thus, greater than 90% of the particles were of fungal origin. The extracts strongly inhibited protein synthesis and thymocyte proliferation. Purified satratoxin H was also highly toxic in the same systems. Each of the individual filters contained satratoxin H (average, 9.5 ng/mg of dust). Satratoxin G and trichoverrols A and B were found in lesser amounts in some, but not all, of the filters. The limit of analysis is ca. 50 ng. These results establish that the conidia of S. atra contain trichothecene mycotoxins. In view of the potent toxicity of the trichothecenes, the inhalation of aerosols containing high concentrations of these conidia could be a potential hazard to health.

Aerosols↗
PubMed1986

Two apparent Philadelphia chromosomes arising from translocations with different chromosomes in a patient with CML: 46,XY,t(7;22)(p22;q11),t(9;22)(q34;q11).

Chromosome studies on bone marrow cells and unstimulated peripheral lymphocytes from a patient with chronic myelogenous leukemia revealed the presence in all cells of two apparent Philadelphia chromosomes: one resulting from the classical translocation with a chromosome #9, and the other arising from a translocation between chromosomes #22 and #7. There was no normal chromosome #22. Some of the cells also had an i(17q), indicative of blast crisis. Repeated chromosome studies at different times during the course of the disease revealed the evolution of additional karyotypic changes. All cells from later samples had an extra #8; some of these cells had a third Philadelphia chromosome, whereas, others had a second Y chromosome. Although a few normal cells were seen in PHA-stimulated lymphocyte cultures, indicating that the patient has a normal constitutional karyotype, most of the cells had a karyotype identical to that found in unstimulated cultures. This unusual karyotype, 46,XY,t(7;22)(p22;q11),t(9;22)(q34;q11), represents the first case in which two apparent Philadelphia chromosomes are present in the leukemic cells from a patient in the absence of a normal #22 chromosome.

Chromosome Banding↗