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Biomedical subjects

J Simon

Publications and source records attributed to J Simon.

At least 451 records · Page 25Linked to original sources
PubMed1987

Pulmonary bacterial deposition and clearance during ascarid larval migration in weanling pigs.

Pulmonary deposition and clearance of bacteria were measured in weanling pigs, half of which had been inoculated at age 31 days with larvated Ascaris suum ova. Seven days later, when breathing signs of larval migration were pronounced, all pigs were exposed to aerosolized Escherichia coli (strain B). Then, either immediately after aerosol exposure (for deposition assessment) or immediately after a 120 minute period in filtered air (for clearance), bacteria in the pigs' lungs were counted. Ascarid ova-inoculated pigs did not differ significantly from control pigs for number of bacteria in the lungs after aerosol exposure, but after the 120 minute clearance period they had 7.2 times more than did the control pigs. Thus, in weanling pigs, the breathing-pattern changes that were evident during ascarid-larval migration did not affect pulmonary deposition of inhaled bacteria significantly, but the presence of ascarid larvae in the lungs was associated with impaired pulmonary bacterial clearance.

Animals↗
PubMed1987

Skeletal localization of samarium-153 chelates: potential therapeutic bone agents.

A series of stable complexes of 153Sm has been produced using multidentate acetate and phosphonate ligands. Biodistribution studies in unanesthetized rats showed varying degrees of bone and soft-tissue uptake for these complexes. Of the complexes studied, [153Sm] ethylenediaminetetramethylenephosphonate (EDTMP) showed the best combination of high bone uptake, low nonosseous uptake, and rapid blood clearance which warranted its further investigation in rabbits. Rabbit studies confirmed the [153Sm]EDTMP results obtained in rats. Blood clearance in rabbits was found to be more rapid than [99mTc] methylene diphosphonate (MDP). Scintigraphic images were virtually indistinguishable from [99mTc]MDP images. Lesion/normal bone ratios were determined from digitized images obtained using a drill hole model and found to be approximately 17:1. Based on these excellent biodistribution characteristics, [153Sm] EDTMP could be therapeutically useful in treating metastatic bone cancer.

Animals↗
PubMed1987

[The course of renal function in vesicorenal reflux. Study using I-123 Hippuran].

Fifty-two patients with vesicoureteral reflux (age: 6.4 +/- 6.2 years) were examined by IVP, voiding cystouretrography and 123 I-Hippuran studies. 17 patients were not operated; 26 had an antireflux, 9 an urethral surgery. The follow-up was 2 years for 39 of these patients, with at least one evaluation per year. The observations were: Unoperated patients were younger, had a lower radiological grade of reflux and renal functional impairment was less frequent. Functional impairment was initially equally distributed in unilateral (53%) and bilateral (54%) reflux. Radiological grade of reflux had an unsatisfactory predictive value for functional renal status. Functional renal status at 2 years was unchanged in 82% of patients. Improvement was observed in 8%, deterioration in 10%. These results were not influenced by the choice of treatment. However, reflux persistence was more frequently observed in the un-operated group (50% versus 5%). Renal transit times of 123 I-Hippuran did not correlate with the functional status or the radiological grade of reflux.

Adolescent↗
PubMed1986

Fasting and refeeding alter the insulin receptor tyrosine kinase in chicken liver but fail to affect brain insulin receptors.

Insulin receptors from chicken liver and brain were studied following alterations in the nutritional state. Chickens were either fasted for 48 h, fasted for 48 h and then refed for 24 h, or fed a regular diet ad libitum. 125I-Porcine insulin binding was significantly elevated in liver membranes from the fasted animals and lowered in refed chickens when compared to preparations from ad libitum fed chickens. These changes in 125I-insulin binding were inversely related to the levels of plasma insulin and since receptor affinities for insulin were similar in each group, they probably represent alterations in receptor number. Apparent Mr of alpha subunits of the insulin receptors was unaffected by alterations in the nutritional states. The presence of ATPase-like activities that co-eluted with liver insulin receptors from wheat germ agglutinin lectin columns but not from pea lectin columns necessitated the use of both pea and wheat germ agglutinin for liver insulin receptor purification. The insulin receptors purified from both lectin columns were recognized by anti-insulin receptor antiserum and had similar affinities for insulin which were unaltered by the nutritional state. Insulin-stimulatable autophosphorylation of the beta subunit of the insulin receptor was lower in livers from fasted chickens and intermediate in refed chickens. Furthermore, basal and insulin-induced phosphorylation of the artificial substrate poly(Glu,Tyr) 4:1 was significantly less in the fasting state and intermediate in the refed state compared to the ad libitum fed state. Insulin sensitivity (measured as the dose of insulin required for 50% maximal stimulation of kinase activity) was similar in all three states suggesting that the differences in insulin-induced phosphorylation are due to a change in maximal stimulation and not a change in insulin sensitivity. In contrast to the alterations seen with liver receptors, brain insulin receptors were unaffected by these alterations in nutritional state. These findings suggest that: liver insulin receptors are affected by altering the nutritional state; insulin binding to liver membranes is inversely related to plasma insulin levels; and tyrosine kinase is decreased both in fasted and refed animals suggesting an uncoupling of the normal interaction between alpha subunit and beta subunit in liver insulin receptors.

Animals↗
PubMed1986

Agonist and blocking effects of choline at the neuromuscular junction.

The effects of choline chloride were studied at the voltage-clamped frog neuromuscular junction by measuring miniature endplate currents and equilibrium dose response curves for acetylcholine applied by microionophoresis. Choline reduced the amplitude and shortened the time constant of miniature endplate currents in a dose dependent manner. Dose response curves carried out in the presence of low doses of choline (200 microM) were shifted to the right and the apparent dissociation constant for ACh was increased without affecting the Hill coefficient or the maximum conductance at the endplate. Higher doses of choline shifted the curve even further to the right but reduced the Hill coefficient and maximum conductance. Choline ionophoretic dose response curves were carried out but the conductance response was only about 1% of the response to comparable concentrations of ACh. In the presence of ethanol, which reduces the agonist dissociation constant, choline responses were increased and the dose response curve analysis revealed that the efficacy of choline was about 17% in comparison to ACh. Similar effects were measured at rat endplates. Rat nerve-muscle preparations were used to investigate the effects of choline upon neuromuscular transmission.

Acetylcholine↗
PubMed1986

Insulin receptors of chicken liver and brain. Characterization of alpha and beta subunit properties.

Receptors on membranes of chicken liver and brain bound porcine 125I-insulin in a specific and temperature-dependent manner. Competition with unlabeled insulin derivatives exhibited typical insulin potency ratios, i.e. chicken greater than porcine insulin greater than human proinsulin (2.1/1/0.02). Apparent binding affinity was higher in brain with a 50% inhibition of tracer binding of 1.3 +/- 0.2 nM porcine insulin as compared to 2.8 +/- 0.3 nM in liver. The apparent molecular mass of the 125I-insulin cross-linked alpha subunit of the insulin receptor was 139 +/- 2 kDa for chicken liver and 127 +/- 2 kDa for chicken brain. These molecular masses were similar to those of rat liver and brain insulin receptors. Neuraminidase treatment of the cross-linked insulin receptor increased the mobility of the alpha subunit from liver but did not affect that from brain, suggesting a difference in the glycosylation of the chicken brain alpha subunit as previously described in the rat. Despite this change, both receptors could be purified on wheat germ agglutinin (WGA) chromatography after Triton solubilization. In the presence of CTP and vanadate (phosphatase inhibitors) insulin-stimulatable tyrosine-specific phosphorylation of exogenous substrates was demonstrated with chicken liver and brain receptors. The reaction was dependent on Mg2+ and Mn2+. As noted with other insulin receptors, the best artificial substrate for phosphorylation was poly(Glu,Tyr)4:1. In both chicken liver and brain the smallest effective insulin dose as well as maximal stimulation of phosphorylation of the substrate was similar to that seen with rat liver, and in all three tissues chicken insulin was more potent than porcine insulin. In chicken liver an active ATP hydrolytic activity copurified with the insulin receptors during WGA chromatography. Further purification using S-300 Sephacryl filtration or affinity (insulin-biotin-avidin) chromatography could dissociate the phosphorylation and the hydrolytic activities. Gel electrophoresis, under reducing conditions revealed beta subunits with apparent Mr of 97-99 kDa in chicken liver and brain, which were phosphorylated in the presence of insulin. Similar apparent molecular masses have been described for the beta subunit of rat liver receptors. These studies suggest that both chicken brain and liver insulin receptors exhibit coupling of alpha and beta subunits with fully active tyrosine kinase and that the structural difference of the brain insulin receptor is widespread and phylogenetically old.

Adenosine Triphosphate↗
PubMed1986

Joint angle signaling by muscle spindle receptors.

Nerve impulses were recorded from sensory fibers supplying the tibialis anterior and soleus muscles of anesthetized cats as the ankle joint was moved from one end of the flexion-extension axis to the other and back again in steps of 6-7 degrees. The rate of movement from one position to the next was 40 deg/s and each position was held for 16-18 s. Plots were made of receptor discharge frequency as a function of ankle joint angle during joint movement (dynamic input-output (I-O) functions) as well as 2 and 15 s after movement terminated (2 and 15 s static I-O functions). Only receptors with a sustained (5s) static response within the physiological range were studied. A total of 229 tibialis anterior receptors met this criterion, of which 11 were identified as tendon organs. One hundred and five soleus receptors were studied, of which 6 were tendon organs. Thus tendon organ activity accounted for only a small part of the muscle afferent signal under passive conditions. The spindle receptors in soleus and tibialis anterior divided the ankle flexion-extension range about equally between them, those in soleus signaling over the flexion half of the range and those in tibialis anterior over the extension half. At angles where the receptors in a particular muscle did not signal joint angle, the tendon of the muscle was observed to be slack. Thus the total muscle afferent discharge in a relaxed animal is high at one end of the range, declines progressively as the ankle is displaced to an intermediate position, and then increases again as the joint moves toward the opposite end of the range. The spindle receptors within an individual muscle were recruited rather early as the muscle came under tension so that over most of a muscle's signaling range joint angle could have been coded by changes in receptor discharge frequency but not by which spindle receptors were active. To evaluate the information signaled by individual muscle spindle receptors, the following measurements were made from plots of impulse frequency vs joint angle: dynamic response, defined as the frequency difference between the dynamic and 2 s static I-O functions during muscle lengthening; adaptation, defined as the frequency difference between the 2 and 15 s static I-O functions during muscle lengthening.(ABSTRACT TRUNCATED AT 400 WORDS)

Adaptation, Physiological↗
PubMed1986

Increased effect of multiple doses of 40 749 RP a new long acting gastric antisecretory agent.

40,749 RP, a pyridyl-2-tetrahydrothiophene derivative is known to be a potent gastric antisecretory agent in animals and man. In 27 healthy volunteers in four double-blind cross-over studies, this activity has been examined against basal and pentagastrin stimulated gastric acid secretion. The effect of a single oral dose of 2 mg/kg was still present after 12, 24 and 48 h. The effect of 1 mg/kg was definitely increased after treatment for 7 days as compared to a single dose of 1 mg/kg.

Adolescent↗
PubMed1986

Cabanas approach: is sentinel node biopsy reliable for staging penile carcinoma?

In 1977 Cabanas proposed biopsy of a sentinel node which is considered the primary site of metastasis from penile carcinoma. If this node is not invaded by tumor, no further surgical treatment is necessary. We report on 2 patients in whom results of bilateral sentinel lymph node biopsies were negative and in whom pelvic lymph node metastasis developed within one year.

Aged↗
PubMed1986

Hydrodynamic parameters of opioid receptors from frog brain.

Active opioid receptors were solubilized from frog (Rana esculenta) brain membranes using 1% digitonin. The solubilized preparation was sedimented in sucrose density gradient and applied to Sepharose-6B column. In the ultracentrifugation experiments, two distinct molecular forms of the opioid receptors were observed with apparent s20, w values of 15.7 and 10.8 S. The estimated molecular weights were 470 and 180 kD. The Stokes radii of the two separate forms were determined by gel filtration and found to be 71 and 42 A. The corresponding molecular weights were 500 and 140 kD indicating a good correlation with data obtained from the sedimentation experiments.

Animals↗