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Biomedical subjects

J Silver

Publications and source records attributed to J Silver.

At least 415 records · Page 23Linked to original sources
PubMed1976

Pyorrhoea as cause of pyrexia.

Three patients with fever and malaise, one of whom also had joint pains, were extensively investigated before their condition was attributed to dental sepsis. Each patient recovered fully after appropriate dental treatment. Dental sepsis should be added to the list of possible causes of pyrexia of undetermined origin, and a routine dental examination should be carried out in each case.

Adult↗
PubMed1976

Preliminary amino acid sequences of transplantation antigens: genetic and evolutionary implications.

Preliminary amino acid sequence data on the transplantation antigens of mouse and man have led to provocative hypotheses about the genetic organization and evolution of genes coded by the major histocompatibility complex of mammals. New microsequencing techniques should permit a detailed analysis of these gene products and an eventual choice among the alternative hypotheses now posed. These data have made it apparent that the H-2 complex is a fascinating and complicated chromosomal region which will continue for some time to intrigue immunologists, geneticists, biochemists, and cell biologists.

Alleles↗
PubMed1976

Structure and evolution of transplantation antigens: partial amino-acid sequences of H-2K and H-2D alloantigens.

Techniques for the amino acid sequence analysis of subnanomole quantities of polypeptides have been applied to characterize beta2-microglobulin and transplantation antigens of the mouse isolated from spleen cells by indirect immunoprecipitation. Eleven residues were identified throughout the NH2-terminal 27 residues of the beta2-microglobulin; all were identical to residues seen at the corresponding positions of beta2-microglobulins from other species. Two K and two D transplantation antigens were examined and the following generalizations emerged from the limited partial amino-acid sequence data: (1) the K and D molecules are homologous to one another; (2) they do not show amino acid sequence homology with immunoglobulins; (3) the two K and two D molecules differ from one another by multiple amino acid substitutions; and (4) the K molecules as a class cannot be distinguished from the D molecules as a class. The genetic and evolutionary implications of these observations are discussed.

Amino Acid Sequence↗
PubMed1976

An autoradiographic analysis of [3H]alpha-bungarotoxin distribution in the rat brain after intraventricular injection.

Purified alpha-bungarotoxin was isolated by chromatography and made radioactive with tritium ([3H]acetamidino-alpha-bungarotoxin). Infusions of [3H]alpha-bungarotoxin alone or preceded by tubocurarine or atropine were given into the third ventricle. 2. 12, or 24 h after injection the brains were prepared for autoradiography. Injections of alpha-bungarotoxin (radioinert) in buffer, or of [3H]parathyroid hormone in buffer, served as controls. The various patterns of labeling suggest the presence of nicotinic-cholinergic neurons within the arcuate and basolateral regions of the hypothalamus including the supraoptic and suprachiasmatic nuclei and, in addition, the central nucleus of the amygdala.

Amygdala↗
PubMed1976

New approaches to the chemical characterization of membrane molecules.

Recognition of the H-2D and H-2K cell surface molecules plays an integral role in the cell mediated cytolysis of virally infected or chemically modified cells by immune cytotoxic thymus derived cells. Previously, technical difficulties have made structural studies on these membrane molecules extremely difficult. We present micromethods based on detergent solubilization and indirect immunoprecipitation which have allowed us to determine the amino acid sequence of the N-terminal portion of four H-2D and H-2K molecules. Application of these microsequencing methods to the determination of the detailed structure of these molecules will allow us to assess their involvement as targets in immune surveillance. This kind of approach will facilitate detailed chemical analysis of a variety of membrane molecules of interest in developmental and tumor biology.

Alleles↗