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J Sikorová

Publications and source records attributed to J Sikorová.

16 recordsLinked to original sources
PubMed1988

Remarks on small sample size taken from reference population for examination of factor VIII.

Investigation of plasma factor VIII activities in 20 reference (normal) individuals (N = 20) is frequently used to evaluate the reference distribution parameters by means of simple statistical method. Two examples demonstrate the inaccuracy of conclusions of such a small sample of data. The first one shows the comparison of estimate of F.VIII:C reference limits obtained from examination of 6 different groups randomly chosen (N = 20 each) with a group of N = 120 reference individuals. The second example presents the balance of haemophilia A carrier detection rate performed in 30 obligatory carriers and 42 normal women by examination of F.VIII:C and VWF-Ag using universal discriminant. The correction figures typical for specific conditions of our laboratory are obtained from examination of N = 20 and N = 80 individuals and using heuristic optimalization.

Adolescent↗
PubMed1987

[Lyophilized plasmas for the control of thromboplastin time values and calibration of thromboplastins].

The paper deals with the production of normal and pathological plasmas for the calibration of thromboplastin. Calibration is performed according to WHO principles. We compared single charges of thromboplastin (Sevac, CSSR, human brain) with each other and a selected reference charge with British thromboplastin. Lyophilised reference plasmas produced (20-50 healthy blood donors) and plasmas taken from patients treated with coumarin derivates (single and mixtures) could replace their fresh equivalents which was confirmed by testing 4 different batches. The stability of these plasmas (for a time of at least 12 months) was proved by repeated checks. These plasmas also served as controls in other laboratories in Czechoslovakia.

Blood Coagulation Tests↗
PubMed1980

Lyophilised plasmas for one-stage determination of factor VIII.

Two batches of lyophilized normal pooled HEPES-stabilized plasma according to ZUCKER [11] and LOELIGER [7] were prepared. The F VIII activity established by the activated partial thromboplastin test (APTT-VIII) was identical in both plasmas and amounted to 0.99 U/ml (95% confidence limits 0.87 to 1.17 U/ml). It did not change within 11 months in plasmas according to ZUCKER, and 6 months in those according to LOELIGER (further on not followed). We prepared four batches of lyophilized HERPES-stabilized substrate plasma (severe form of hemophilia A) according to ZUCKER. They were employed for 5 to 13 months in APTT-VIII along with the frozen 3.2% sodium citrate substrate plasmas. The results showed a satisfactory agreement. The lyophilized plasmas did not influence even the dose-response-line. On the ground of these results we consider the reported lyophilized plasmas to be suitable reagents in APTT-VIII technique.

Blood Coagulation Tests↗