Metabolic products of fungi. XXIV. The structure of erythroskyrine, a nitrogen-containing coloring matter of Penicillium islandicum Sopp.
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Chemical Phenomena↗
Biomedical subjects
J Shoji
Publications and source records attributed to J Shoji.
Immunosuppressive effect of cholera toxin B on allergic conjunctivitis model in guinea pig.
PURPOSE: To investigate the new method of immunotherapy using cholera toxin B (CTB) in experimental allergic conjunctivitis. METHODS: We used 21 white Hartley guinea pigs. The animals were sensitized by intraperitoneal injection of ovalbumin (100 microg/mL) and albumin hydroxide (5 mg/mL) repeated after an interval of 2 weeks. One week after the second injection, conjunctivitis was induced by topical instillation of ovalbumin (5 mg/mL). The animals were divided into two groups, CTB group and control group. The CTB group underwent pretreatment of topical instillation of CTB (4 microg/30 mL) and ovalbumin (10 microg/30 mL), three times a day for 3 days, 1 week before the intraperitoneal injection. The control group did not undergo the pretreatment. Clinical examination was performed at 0.5, 6, and 24 hours after the development of conjunctivitis. Histological examination was performed at 6 and 24 hours. RESULTS: Both groups developed palpebral and bulbar edema with hyperemia 30 minutes after instillation of ovalbumin. The allergic reaction score was significantly less in the CTB group than in the control group (Mann-Whitney U-test: P <.01). The clinical reactions subsided after 6 hours. The CTB group showed less eosinophilic infiltration in the conjunctiva and the limbus, particularly in the conjunctival epithelium, than the control group at 6 and 24 hours. CONCLUSION: Pretreatment with topical CTB and antigen suppresses clinical and histological findings in experimentally induced allergic conjunctivitis.
Animals↗
Immunohistochemical study on follicular dendritic cell of conjunctiva-associated lymphoid tissue.
We performed an immunohistochemical study of follicular dendritic cells (FDC) in the follicular area of the conjunctiva-associated lymphoid tissue (CALT). Hartley guinea pigs were sensitized with a topical application of an emulsion of ovalbumin and Freund's complete adjuvant in the eye. They were divided into four groups. The control (group A) underwent no sensitization. The sensitized animals were studied at 1 week (group B1) or 2 weeks (group B2) after the sensitization. Additional sensitization at 1 week after the initial sensitization was also performed (group C). Histological methods included methylgreen pyronine staining, alpha-naphthylacetate esterase staining, and enzyme-antibody method against S-100 protein. The uptake of topically applied peroxidase-anti-peroxidase (PAP) in CALT was also examined histologically. In each group, positive reticular patterns by alphanaphthyl acetate esterase staining and immunoperoxidase staining with anti-S-100 protein antiserum were found in the CALT follicular area. The positively stained cells were found to be dendrite cells by immunoelectronmicroscopy. An uptake of PAP was found in the CALT follicular area, suggesting the function of trapping and retaining antigen-antibody complex by FDC. It was concluded that dendritic cells in the CALT follicular area were identified to be FDC.
Animals↗