The therapeutic ways for chronic liver diseases accompanied by diseases of the endocrine and mammary glands.
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Addison Disease↗
Biomedical subjects
J Shi
Publications and source records attributed to J Shi.
Actions of bovine plasma fibronectin on cultured human dental pulp cells.
OBJECTIVE: Fibronectin (FN) has been shown to play a crucial role in odontoblast differentiation, and it is involved in the repair of dentine and pulp. The responsiveness of human dental pulp (HDP) cells to bovine plasma FN was investigated. MATERIALS AND METHODS: After isolation and selection noncarious freshly extracted third molars in vitro, HDP cells were incubated for 72 hours one of four concentrations (10, 20, 40, 80 micrograms/ml) of bovine plasma FN with Dulbecco's modified eagle medium (DMEM) containing 1% fetal calf serum (FCS). We examined cell proliferation (using an MTT assay), DNA synthesis (using incorporation of [3H]-thymidine), the cell area, and alkaline phosphatase (ALPase) activity of the culture supernatant. The expression of FN, types I and III collagens, binding of lectins concanavalin(ConA), or wheat germ agglutinin (WGA) in vitro was examined by means of immunocytochemistry. The expression degree and the cell area were analyzed semi-quantitatively with an image processing and analysis system. RESULTS: After 72 hours, HDP cells appeared contracted and smaller in the absence of FN when compared to cells incubated with FN. HDP cells were in spread state with all four concentrations of FN. FN (20, 40 micrograms/ml) stimulated cell proliferation, promoted incorporation of [3H]-thymidine, and increased the levels of ALPase activity in supernatant. The binding of ConA, the expression of type I collagen, and FN were significantly increased, while the binding of WGA, and the expression of type III collagen were significantly decreased in the FN (40, 80 micrograms/ml) groups when compared to the FN (10, 20 micrograms/ml) groups. CONCLUSION: These findings suggest that HDP cells utilize FN for attachment and subsequent spreading. FN might have mitogenic activity and some role in the regulation of differentiation of pulp cells into odontoblasts.
Alkaline Phosphatase↗
[The expression of TGF-beta receptors on cultured human dental papilla cells].
OBJECTIVE: To observe the expression of TGF-beta receptors on cultured human dental papilla cells and the effects of TGF-beta on TGF-beta receptors of the cells. METHODS: The expression of TGF-beta receptors on cultured human dental papilla cells and the effects of TGF-beta on TGF-beta receptors of the cells were examined by cell culture and immunohistochemical technique and image analysis. RESULTS: Type I, type II receptors for TGF-beta in human dental papilla cells were stained strongly and type III was weakly positive. Image analysis indicated that all experimental groups had no obvious difference with control groups (P > 0.05). CONCLUSIONS: Cultured human dental papilla cells express TGF-beta type I, type II and type III receptors. TGF-beta has no obvious effects on the cell receptor expression.
Activin Receptors, Type I↗
[Dynamic changes in dermal cell cycles, contents of hydroproline and ratios of collagen type I/III in the burned wound].
OBJECTIVE: To study the dynamic changes in the dermal cell cycles contents of hydroproline and ratios of collagen type I/III in the burned wound. METHODS: The dermal cell cycles, contents of hydroproline, ratios of collagen type I/III and pathologic changes were dynamically observed in 48 deep partial burn rats (TBSA 10%), attempting to investigate the process of deep partial burn wound healing. RESULTS: The percentage of S-phase reached the highest point on PBD 10. On PBD21, it was still significantly higher than that of normal group. OHP increased gradually after burn, and peaked on PBD14. On PBD21, the level of OHP was still significantly higher than that of normal group. The ratios of collagen type I/III decreased gradually and significantly lower than those of the normal group after burn. The strips of WBC infiltrating into wound were observed on PBD3, 7 and 10. On PBD 14, a large number of regenerative epidermal cells crept over wound surface. CONCLUSIONS: The inflammation is obvious from PBD3 to PBD10. The proliferation of dermal cell and the synthesis, deposition of new collagen are predominant from PBD10 to PBD14, and the repair of dermis is not complete on PBD21. The proliferation and migration of epidermal cells are major activities from PBD14 to PBD21.
Animals↗
[Analysis on Helicobacter pylori infection and its related diseases in 1,442 children in Tianjin city].
OBJECTIVE: The aim of this study was to investigate the prevalence of Helicobacter pylori (Hp) infection in children in Tianjin and to study the relationship between the Hp infection and its related diseases. METHODS: (1) Serologic examination for Hp - IgG; (2) Rapid urease test for Hp (Hp - Rut); (3) Giemsa staining for Hp; (4) Gastric fluid examination for Hp by PCR. Hp infection were diagnosed by at least two criteria. RESULTS: (1) Of the 1 442 children, 651 were Hp - IgG positive. The total positive rate was 45.15%, Positive rates of Hp - IgG among children under the age of 1 year, 3 years, 6 years, 10 years and to 14 years were 11.19%, 19.23%, 44.21%, 54.20% and 61.46%, respectively. (2) Positive rates of Hp - IgG were 31.18% (164/526) and 53.17% (48/916) in asymptomatic children and in patients with gastrointestinal symptoms. The rate was significantly higher in the later group (P < 0.005). (3) Hp infection rate in patients with duodenal ulcer (DU) was 90.76%, higher than that in other gastrointestinal diseases. (4) Histological examination showed that Hp infection rates were 33.20% and 83.97% in mild and moderate or severe gastritis, respectively. CONCLUSIONS: (1) The prevalence of Hp infection in children in Tianjin was high. (2) Rate of Hp infection increased with age and Hp infection rate was higher in children with gastrointestinal symptoms than with asymptomatie. (3) There was a high correlation in children with gastroduodenal diseases and Hp infection especially with DU. (4) Hp positive rate correlated with the pathohistologic severity of gastritis.
Adolescent↗
[The effect of nordihydroguaiaretic acid on endothelial cell migration induced by glioma cells].
OBJECTIVE: To investigate the effect of nordihydroguaiaretic acid (NDGA) on the migration of endothelial cells (ECs) induced by glioma cells (GCs) in vitro. METHODS: Cells of human umbilical vein endothelial cell line ECV-304 and human malignant glioma cell line SHG-44 were co-cultured in the Falcon Cell Culture Insert system, and the effect of NDGA on the migration of ECs induced by GCs was investigated. The expression of vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) was examined with immunohistochemistry. RESULTS: The expressions of VEGF and bFGF in SHG-44 cells were reduced after treatment with 100 micromol/L NDGA for 1 to 3 days. 100 micromol/L NDGA significantly inhibited not only the chemotactic migration of ECs induced by either glioma cells or glioma-conditioned media, but also the random motility of ECs (P < 0.01). NDGA at a concentration between 50 micromol/L and 200 micromol/L inhibited the chemotactic migration of ECs in a dose-dependent manner (the inhibition ratios were 12.0%, 37.4%, 86.7% and 82.2%). CONCLUSION: The results confirm the inhibitory effects of NDGA on the migration and angiogenic factor expression of ECs, which suggests that NDGA may suppress angiogenesis of glioma.
Brain Neoplasms↗
[Inhibition of hTR activity for suppression of lung cancer cell proliferation using antisense phosphorothioate oligonucleotides].
OBJECTIVE: Telomerase activity was measured in 8 lung tumor cell lines and transfected antisense phosphorothiate oligodeoxynucleotides (anti-PS-ODN) of hTR as therapeutic agents into target cell LTEP-a-2 to investigate the inhibitory effect on telomerase activity and tumor cell growth. METHODS: 1. Telomerase activity assay in 8 human lung tumor cell lines using telomerase PCR ELISA. 2. Synthesized anti-PS-ODNs of hTR and random PS-ODN transfected with Clonfectin into LTEP-a-2 cell lines for 72 hr. 3. Measure telomerase activity by telomerase PCR ELISA, SDH activity by MTT assay and cell growth. RESULTS: Eight cell lines showed positive expression of telomerase activity. Various anti-PS-ODNs could inhibit telomerase activity, SDH activity and cell growth. The inhibition became more marked with the increase of anti-PS-ODNs concentrations. Concentrations of 5 - 40 micromol/L anti-PS-ODNs of hTR specifically reduced the telomerase activity by 25.7% - 84.0%. PS-ODNs of anti-hTR were also able to reduce SDH activity by 19.4% - 74.7% at 5 - 40 micromol/L. The dose of 5 - 40 micromol/L PS-ODNs of anti-hTR had the ability to inhibit cell growth by 14.8% - 72.5%. The results indicate that random sequence of PS-ODN(9) failed to inhibit telomerase, SDH activity and cell growth. A statistically significant difference exists between random PS-ODN and three anti-PS-ODNs (P < 0.01). CONCLUSIONS: Human lung tumor cell lines express high telomerase activity. PS-ODNs of anti-hTR had the ability to inhibit telomerase and reduce LTEP-a-2 cell growth and metabolism.
Adenocarcinoma↗
[Effects of hypoxia alone or exercise combined on capillarization of rat gastrocnemius muscle and its mechanism].
OBJECTIVE: To study the effects of hypoxia and hypoxia-combined-exercise on capillary density and vascular endothelial growth factor (VEGF) and its receptor KDR of skeletal muscle in rats. METHODS: Myosin-ATPase histochemistry was used to assay the size and capillary density of skeletal muscle. VEGF and its receptor KDR were studied by immunohistochemistry. RESULTS: Five-week hypoxia (simulated 5,000 m altitude) resulted in a decrease in cross-sectional area of skeletal muscle fiber and an increase in capillary density (CD), but the capillary/fiber ratio (C/F) remained unchanged. After 5-week-exercise at high altitude (1 h/d, 6 d/w), the muscle fibers did not undergo atrophy. At the same time, CD and C/F were increased. VEGF protein was found primarily in the matrix between muscle fibers; VEGF receptor-KDR was shown mainly in endothelial cells of capillary. VEGF was more strongly stained in the skeletal muscle of hypoxia-combined-exercise rats than the other two groups. CONCLUSION: Hypoxia itself can not induce neovascularization, while hypoxia-combined-exercise rats show capillary proliferation in skeletal muscle. VEGF and its receptor might play roles in this process.
Animals↗
[Study on clinical and laboratory features of preleukemia patients].
OBJECTIVE: To explore prospective diagnostic criteria for preleukemia. METHODS: A case control study of the discrepancies of clinical and laboratory features between patients with preleukemia and those with chronic aplastic anemia (CAA) or atypical paroxysmal nocturnal hemoglubinuria (a-PNH). RESULTS: There were eight variables of significance: (1) lymphocytoid micromegakaryocytes in marrow; (2) immature granulocytes in peripheral blood; (3) >or= 2% myeloblasts in marrow; (4) positive periodic acid schiff (PAS) staining of nucleated erythrocytes; (5) myeloid differentiation index >or= 1.8; (6) clonal karyotypic abnormalities; (7) negative sister chromatid differentiation; (8) > 4.0 cluster/colony ratio of granulocyte-macrophage colony-forming units (CFU-GM). The following criteria was assigned: A: To meet (1) and at least two of the other seven variables; B: To meet at least four of the eight variables. All of the patients with preleukemia met A or B and none of the patients with CAA or a-PNH did. CONCLUSION: Preleukemia is different from CAA or a-PNH. It has its own clinical and laboratory features, which may be useful for prospective diagnosis.
Adolescent↗
[Separation and determination of tyramine by reversed-phase high performance liquid chromatography].
The method for separation and determination of tyramine by reversed-phase high performance liquid chromatography has been established. The effects of mobile phase on mechanism of separation based on the ion interaction are discussed. Using C8 alkyl bonded phase and 40% methanol-water solution containing Trisperchlorate as pH buffer (adjusted pH to 7.9) and ion interaction reagent and applying p-toluene sulfonamide as internal standard substance, the tyramine obtained from decarboxylation of p-tyrosine was determined. The RSD of determination was 0.66%(n = 11). The recoveries of sample were between 99.33% and 100.38%.
Alkylation↗
[Studies on pharmacokinetics of chloroquine in mice infected with chloroquine-resistant strain of Plasmodium berghei].
OBJECTIVE: To compare the pharmacokinetic differences of chloroquine in normal mice and the mice infected with the N and the RC strains of Plasmodium berghei. METHODS: The concentrations of chloroquine in the plasma of normal mice and the mice infected with the N or the RC strains of P. berghei were analyzed by reverse-phase HPLC. The pharmacokinetic parameters were measured with software 3P87. RESULTS: The t1/2 beta value was significantly lower in the mice infected with the RC strain than in normal mice and the mice infected with the N strain(P < 0.05), however, there were no significant differences between the mice infected with the N strain and normal mice. CONCLUSION: Elimination of chloroquine in the mice infected with the RC strain of P. berghei speed up significantly comparing with the mice infected with the N strain.
Animals↗
[Open operation of the vertical condylar fracture].
OBJECTIVE: This article is mentioned to study the incidence, diagnosis, management of the vertical condylar fractures. METHODS: 11 patients, 14 cases of the vertical condylar fractures, were treated in our department between June 1997 and August 1998. All the patients were diagnosed through history, physical examination, radiographs, computed tomography scan and three-dimensional reconstruction of a computed tomography scan, treated with various surgical approaches. RESULTS: All the patients had got satisfactory postoperative result in the aspects of occlusion, degree of opening, joint noise, pain in the area of temporomandibular joint, deviation in mandibular motion and healing of the incision. CONCLUSION: Enough attention should be paid to the vertical condylar fractures. The key to a successful open operation is the correct diagnosis, the accurate reduction and the stable fixation.
English Abstract↗
Reactivity of glutaredoxins 1, 2, and 3 from Escherichia coli shows that glutaredoxin 2 is the primary hydrogen donor to ArsC-catalyzed arsenate reduction.
In Escherichia coli ArsC catalyzes the reduction of arsenate to arsenite using GSH with glutaredoxin as electron donors. E. coli has three glutaredoxins: 1, 2, and 3, each with a classical -Cys-Pro-Tyr-Cys- active site. Glutaredoxin 2 is the major glutathione disulfide oxidoreductase in E. coli, but its function remains unknown. In this report glutaredoxin 2 is shown to be the most effective hydrogen donor for the reduction of arsenate by ArsC. Analysis of single or double cysteine-to-serine substitutions in the active site of the three glutaredoxins indicated that only the N-terminal cysteine residue is essential for activity. This suggests that, during the catalytic cycle, ArsC forms a mixed disulfide with GSH before being reduced by glutaredoxin to regenerate the active ArsC reductase.
Arsenates↗
Isolation, characterization, and chromosomal location of the mouse enamelysin gene.
Mouse enamelysin (Mmp20), a member of the matrix metalloproteinase (MMP) family of extracellular matrix degrading enzymes, shows a high degree of homology with other MMPs, particularly those of the stromelysin/collagenase subfamilies. It is expressed exclusively in ameloblasts and odontoblasts. The mouse enamelysin gene (Mmp20) is made up of 10 exons spanning approximately 65 kb within the MMP gene cluster at the centromeric end of chromosome 9.
Animals↗
Serum amyloid P is not present in amyloid beta deposits of a transgenic animal model.
Serum amyloid P component (SAP) is associated with amyloid beta (A beta) deposition in Alzheimer disease (AD). Since SAP is exclusively synthesized by peripheral organs, its presence in the brain of AD suggests impairment of the blood-brain barrier (BBB). We studied the association of SAP with A beta deposits in a transgenic mouse model overexpressing beta-protein precursor (betaPP). Both SAP and another extracellular matrix binding protein, basic fibroblastic growth factor bind to the heparinase sensitive sites of A beta deposits in this model. However, no endogenous SAP immunoreactivity was found in the transgenic mouse brain. These results suggest that SAP is not required for A beta deposition, and that this mouse model does not develop the same BBB abnormalities as those seen in AD.
Alzheimer Disease↗
Primary afferent fibers of the pelvic nerve terminate in the gracile nucleus of the rat.
After injection of horseradish peroxidase (HRP) into the pelvic nerve of the rat, a small number of HRP-labeled axon terminals were found in the gracile nucleus. Double labeling experiments were also performed: Fluoro-Gold (FG) was injected into the pelvic nerve, while cholera toxin B subunit (CTb) was injected into the gracile nucleus or dorsal faciculus at the fifth and sixth cervical cord segments ipsilateral to the FG injection. About 5% of FG-labeled neurons were labeled with CTb in the L6-, S1- and S2-dorsal root ganglia ipsilateral to the tracer injection. The results suggest that some primary afferent information from pelvic visceral organs may be directly conveyed to gracile nucleus by the primary afferent neurons.
Afferent Pathways↗
Cell transformation by the superoxide-generating oxidase Mox1.
Reactive oxygen species (ROS) generated in some non-phagocytic cells are implicated in mitogenic signalling and cancer. Many cancer cells show increased production of ROS, and normal cells exposed to hydrogen peroxide or superoxide show increased proliferation and express growth-related genes. ROS are generated in response to growth factors, and may affect cell growth, for example in vascular smooth-muscle cells. Increased ROS in Ras-transformed fibroblasts correlates with increased mitogenic rate. Here we describe the cloning of mox1, which encodes a homologue of the catalytic subunit of the superoxide-generating NADPH oxidase of phagocytes, gp91phox. mox1 messenger RNA is expressed in colon, prostate, uterus and vascular smooth muscle, but not in peripheral blood leukocytes. In smooth-muscle cells, platelet-derived growth factor induces mox1 mRNA production, while antisense mox1 mRNA decreases superoxide generation and serum-stimulated growth. Overexpression of mox1 in NIH3T3 cells increases superoxide generation and cell growth. Cells expressing mox1 have a transformed appearance, show anchorage-independent growth and produce tumours in athymic mice. These data link ROS production by Mox1 to growth control in non-phagocytic cells.
3T3 Cells↗
Murine TIMP-2 gene-targeted mutation.
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Animals↗