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Biomedical subjects

J Sherrod

Publications and source records attributed to J Sherrod.

3 recordsLinked to original sources

Assessing immunization coverage in private practice.

To achieve national health objectives of eliminating most childhood vaccine-preventable diseases by the year 2010, all health care providers will have to improve the immunization rates of their patients. Currently, immunization rates of children 19 to 35 months of age are less than national objectives, suggesting a need for optimized immunization services. A key strategy for improving age-appropriate immunization coverage by health care providers is the assessment of immunization coverage. Because most (62%), immunization services in the United States are delivered in the private sector, a concerted effort in private practice is critical to improving immunization rates. Assessment of immunization coverage of patients enrolled in private practice serves 1) to measure the overall performance of the practice in providing the standard of care, 2) to identify strategies for improving coverage, and 3) to document the quality of health services delivered (report card). Assessment of immunization coverage has been demonstrated in several practice settings to be highly effective in improving immunization rates. All types of physicians should benefit from assessing immunization coverage of their patients. Simple assessment tools are available at no cost to the public and can be obtained by contacting the Centers for Disease Control and Prevention. These tools include a manual self-assessment or a computerized software package (CASA) to fit the needs of the practice.

Centers for Disease Control and Prevention, U.S.↗

Insulin processing and dissociation--effect of temperature and lysosomotropic agents.

Dissociation of intact and degraded insulin from hepatocytes in monolayer culture was examined under conditions in which processing of insulin was altered by either temperature or pharmacologic agents. Conditions which increased insulin degradation or processing decreased equilibrium insulin binding whereas those conditions which inhibited processing increased equilibrium binding. The effect of lysosomotropic agents on processing was markedly temperature dependent. Not only was processing increased at higher temperatures (37 degrees C, but the effect of lysosomal inhibitors (chloroquine and methylamine) on insulin processing was abolished at this temperature. The temperature dependency of this effect may explain discrepancies between laboratories on the effect of these inhibitors in hepatocytes.

Animals↗

Effect of down-regulation of insulin receptors on receptor-antibody binding.

The extraction of insulin receptor antibody (ARAB) by IM-9 lymphoblastoid cells was measured using nonisotopic ARAB, ARAB extraction was expressed as a clearance, with ARAB determinations made indirectly by a method dependent on its inhibition of insulin binding. Control cells and down-regulated IM-9 cells (cells exposed to 10(-7) M insulin for 16 h) at a cell density of 8 X 10(7) cells/ml were incubated in the presence of a 1:75 dilution of serum from a patient with insulin resistance due to insulin receptor antibodies. After 60 min at room temperature, control cells cleared 42 +/- 3% of ARAB compared to only 18 +/- 2% by the down-regulated cells. ARAB clearance was directly proportional to maximal tracer insulin binding, as determined in parallel experiments. ARAB clearance was similarly reduced in a dose-related fashion by 16-h exposure to Concanavalin A. These results indicate that ARAB extraction by IM-9 cells is reduced by chronic exposure to insulin. The observation that ARAB binding to insulin down-regulated cells is reduced despite the ability of ARAB to attach normally to cells whose receptors have been saturated with insulin supports the notion that down-regulation is due to a loss of membrane receptor rather than to simple receptor occupancy. (Endocrinology 108: 478, 1981)

Antibodies↗