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Biomedical subjects

J Sheehan

Publications and source records attributed to J Sheehan.

At least 73 records · Page 4Linked to original sources
PubMed1985

Separation and characterization of two populations of aggregating proteoglycans from cartilage.

Intermediary gel immunoelectrophoresis was used to show that purified aggregating cartilage proteoglycans from 2-year-old steers contain two distinct populations of molecules and that only one of these is immunologically related to non-aggregating cartilage proteoglycans. The two types of aggregating proteoglycans were purified by density-gradient centrifugation in 3.5M-CsCl/4M-guanidinium chloride and separated by zonal rate centrifugation in sucrose gradients. The higher-buoyant-density faster-sedimenting proteoglycan represented 43% of the proteoglycans in the extract. It had a weight-average Mr of 3.5 X 10(6), did not contain a well-defined keratan sulphate-rich region, had a quantitatively dominant chondroitin sulphate-rich region and contained 5.9% protein and 23% hexosamine. The lower-buoyant-density, more slowly sedimenting, proteoglycan represented 15% of the proteoglycans in the extract. It had a weight-average Mr of 1.3 X 10(6), contained both the keratan sulphate-rich and the chondroitin sulphate-rich regions and contained 7.3% protein and 23% hexosamine. Each of the proteoglycan preparations showed only one band on agarose/polyacrylamide-gel electrophoresis. The larger proteoglycan had a lower mobility than the smaller. The distribution of chondroitin sulphate chains along the chondroitin sulphate-rich region was similar for the two types of proteoglycans. The somewhat larger chondroitin sulphate chains of the larger proteoglycan could not alone account for the larger size of the proteoglycan. Peptide patterns after trypsin digestion of the proteoglycans showed great similarities, although the presence of a few peptides not shared by both populations indicates that the core proteins are partially different.

Amino Acids↗
PubMed1985

Fractures of the femoral shaft treated by plating.

Eighty-six fractures of the femoral shaft were treated by open reduction and plating. Seventy-seven fractures (90 per cent) were reviewed 12-72 months (average 38 months) after operation. An excellent or good result was achieved in 70 fractures (91 per cent). There was no case of deep infection. The most common complication was a fatigue fracture of the plate, which occurred in five cases. In patients over the age of 60 years, loosening of the implant was troublesome. Primary bone grafting is essential if rigid internal fixation is not obtained. Plating is an excellent method of treating fractures of the femoral shaft in patients under the age of 60 years, provided that the surgeon is technically competent and experienced in this method of treatment.

Adolescent↗
PubMed1985

The attitudes of nurse tutor students towards behavioural objectives: background and method of study.

Since behavioural objectives are rooted in behaviouristic psychology there is a brief discussion of some notable behaviourists. This is followed by reference to some key characters in the behavioural objectives movement. The strengths and weaknesses of behavioural objectives are discussed with reference to the relevant literature. Behavioural objectives in nursing education are considered and the tensions which their use may give rise to are noted. Finally, details are given of how a small-scale empirical study relating to behavioural objectives was conducted.

Attitude of Health Personnel↗
PubMed1985

The attitudes of nurse tutor students to behavioural objectives: results and discussion.

This small-scale empirical study provides evidence of the attitudes of a group of nurse tutor students to behavioural objectives. The questionnaire items used ranged from questions about behaviourism and the use of behavioural objectives in teaching, to a question on whether the participants would use behavioural objectives in their teaching once they had finished their course. It emerged that while there was not an uncritical acceptance of behavioural objectives, they were seen as having a place in the process of teaching and learning nursing.

Adult↗
PubMed1985

Ethical considerations in nursing practice.

Some ethical implications for nursing practice are considered in relation to three issues: competence, honesty and obedience. Factors which contribute to conformity, obedience and authoritarianism are discussed, and respect for other people is suggested as a guiding principle for ethically acceptable conduct.

Authoritarianism↗
PubMed1983

Isolation and characterization of dermatan sulphate proteoglycan from human uterine cervix.

Proteoglycans were extracted from human uterine cervix with 4 M-guanidinium chloride in the presence of proteinase inhibitors. They were purified by density-gradient centrifugation in 4 M-guanidinium chloride/CsCl (starting density 1.32 g/ml) followed by DEAE-cellulose and Sepharose chromatography. Only one polydisperse proteoglycan was found. s020,w was 2.1S and the weight-average molecular weight was 73 000 (sedimentation-equilibrium centrifugation) to 110 500 (light-scattering). The core protein was monodisperse, with an apparent molecular weight of 47 000. The proteoglycan contained about 30% protein and probably two or three glycosaminoglycan side chains per molecule. High contents of aspartate, glutamate and leucine were found. The glycan moiety of the proteoglycan was exclusively dermatan sulphate, with a co-polymeric structure with approximately equal quantities of iduronic acid- and glucuronic acid-containing disaccharides.

Aged↗