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Biomedical subjects

J Sexton

Publications and source records attributed to J Sexton.

At least 37 records · Page 2Linked to original sources

Children's behavior in emergency and non-emergency dental situations.

This study evaluated children's behavior during emergency and non-emergency visits to the dentist. Three methods were used to evaluate behavior: 1) the Palmar Sweat Index, 2) the Taylor Manifest Anxiety Scale, and 3) the Frankl Behavior Scale. Forty children, 6-12 years of age, were seen in a pediatric dental clinic. All children had no previous dental experiences. Twenty patients were seen for new patient examinations and twenty patients had emergency examinations and treatment. This study showed that children exhibit equal levels of stress whether for a screening appointment or an emergency visit. The Palmar Sweat Index has the potential of being a viable evaluation for detecting non-verbally expressed anxiety.

Child↗

Depression: a possible complication of orthognathic surgery.

Advances in the field of orthognathic surgery as well as increased experience on the part of surgeons have led to successful treatment of greater numbers of patients with maxillofacial deformities. Consequently, greater insight has been gained into not only the technical effects of surgery, but also into the psychological impact on the patients. This paper describes a small pilot study designed to evaluate possible psychiatric effects that may occur during the recuperative period of orthognathic surgery.

Adult↗

Eucaryotic RNA polymerase conditional mutant that rapidly ceases mRNA synthesis.

We have isolated a yeast conditional mutant which rapidly ceases synthesis of mRNA when subjected to the nonpermissive temperature. This mutant (rpb1-1) was constructed by replacing the wild-type chromosomal copy of the gene encoding the largest subunit of RNA polymerase II with one mutagenized in vitro. The rapid cessation of mRNA synthesis in vivo and the lack of RNA polymerase II activity in crude extracts indicate that the mutant possesses a functionally defective, rather than an assembly-defective, RNA polymerase II. The shutdown in mRNA synthesis in the rpb1-1 mutant has pleiotropic effects on the synthesis of other RNAs and on the heat shock response. This mutant provides direct evidence that the RPB1 protein has a functional role in mRNA synthesis.

Chromosome Mapping↗

Epstein-Barr virus in normal pregnant women.

Acquired immune suppression accompanying normal pregnancy may be associated with reactivation of Epstein-Barr virus (EBV). Pregnant women with reactivated EBV having anti-EA antibodies show high titers of antiviral capsid antigen (VCA) geometric mean titers (GMT) of 522 versus 170 in those lacking anti-early antigen (EA). Among twenty-seven seropositive women at parturition, 17 (63%) had generated antibody to EA, and all 27 (100%) demonstrated significant increases in antibody to VCA (p less than 0.01). In contrast, antibody titers to cytomegalovirus, herpes hominis, varicella-zoster, and rubella viruses in the pregnant women were comparable to those found in nonpregnant controls. (Am J Reprod Immunol. 1982; 2:217-221.)

Adult↗

Abnormal anti-Epstein Barr virus antibodies in carriers of the X-linked lymphoproliferative syndrome and in females at risk.

The asymptomatic hemizygous female carriers of the X-linked lymphoproliferative syndrome (XLP) have abnormal antibody responses to EBV. This suggests partial expression of the defect that leads to EBV-provoked life-threatening diseases in their affected sons. EBV specific antibodies were measured in 65 serum samples of 12 obligate carrier females and seven of their daughters (females at risk) during periods ranging from 1 to 5 yr. Abnormal qualitative antiviral capsid antigen (VCA) IgG titers were nearly fourfold higher than normal controls, two carriers had persistent IgM anti-VCA antibody, two-thirds had persistent IgA anti-VCA antibody, and half of the women had titers to early antigen (EA). Five of seven females exhibited a similar persistent pattern. In contrast, none of the unaffected family members nor 23 normal controls expressed IgA or IgM titers to VCA even with high exposure to the virus, and anti-EA was detected in only one control. Therefore, these findings may prove useful for detecting carriers of the syndrome. Abnormal anti-EBV titers similar to the carrier pattern have been reported in patients and other immunosuppressed individuals, and are indicative of active viral infection.

Adolescent↗

Activated human mononuclear phagocytes release a substance(s) that induces replication of quiescent human fibroblasts.

Human mononuclear phagocytes (HMP) treated in vitro with N-acetylmuramyl-L-alanyl-D-isoglutamine (MDP) released a soluble substance(s) that induced in vitro replication of subconfluent, quiescent, serum-free human fibroblasts. The response was enhanced if nonmitogenic plasma-derived serum (PDS) was added to the HMP-conditioned media after harvest. The capacity of serum-free, quiescent fibroblasts to respond to the HMP-released substance(s) diminished with time in culture. The diminished proliferative response was reversed by adding PDS to the MDP-HMP-conditioned media. Similar results were obtained in parallel experiments using platelet-derived growth factor (PDGF). These data suggest that the HMP-associated growth-promoting substances may, like PDGF, act co-ordinately with substances in PDS to stimulate the proliferation of cells in vitro.

Acetylmuramyl-Alanyl-Isoglutamine↗

Surgery in a gravid patient.

A review of salient points in the evaluation and treatment of the gravid patient is presented. Emphasis is on problems arising in the practice of oral surgery. The physiologic mechanisms that form the basis for treatment planning rationale are elucidated.

Abruptio Placentae↗

A new method for rapid fluid bolus infusion into a peripheral vein.

OBJECTIVE: To compare the flow rates achieved by a new short-tubed infusion device with those obtained with a conventional apparatus, using gravity, manual pressure, and pneumatic inflation as the driving forces. METHODS: Ten paramedic volunteers were recruited for this prospective, randomized, controlled laboratory study. For the short-tubing setup, a new device, the port, was used to adapt standard 18-cm extension tubing directly to an i.v. bag [short tubing/port (STP) setup]. For the conventional (CON) setup, 280-cm tubing was used. Both study setups were tested on each of the volunteers with flow from a standard 250-mL bag of normal saline subjected to three types of driving force: 1) gravity alone, 2) pressure supplied by two hands squeezing the i.v. bag, and 3) a pneumatic pressure bag continuously inflated to 300 mm Hg. The mean flow rates for each driving force were compared between the two setups. RESULTS: Using gravity flow, no significant difference was noted between the STP setup and the CON setup (0.40 vs 0.45 mL/sec, respectively, p > 0.4). However, when flow was augmented by pressure supplied by two hands squeezing the bag, mean flow was greater for the STP setup than for the CON setup (4.5 vs 2.7 mL/sec, respectively, p < 0.001). When flow was augmented by a pneumatic bag at 300 mm Hg, mean flow was again greater for the STP setup (5.6 mL/sec) than for the CON setup (3.3 mL/sec, p < 0.001). CONCLUSION: Flow of crystalloid under pressure into a peripheral vein is markedly increased with the new STP setup as compared with the CON setup. Incorporation of this new setup in prehospital care would allow EMS personnel to infuse fluid more rapidly and conveniently during transport.

Adult↗

Adjunctive hyperbaric oxygen therapy reduces length of hospitalization in thermal burns.

The use of adjunctive hyperbaric oxygen therapy as part of a comprehensive program of burn care at our institution has resulted in a statistically significant reduction in length of hospital stay (p = less than 0.012) with no increase in cost of hospital care in patients suffering burns over 18% to 39% of total body surface area.

Adolescent↗

Adjunctive hyperbaric oxygen in the treatment of thermal burns. An economic analysis.

Current reimbursement for burn care underscores the importance of cost containment in the treatment of burn injuries. We evaluate the economic impact of adjunctive hyperbaric oxygen in burns of 19% to 50% total body surface area in patients admitted to our burn center from January 1982 to July 1987. Length of hospitalization, number of surgical procedures, and total cost of care, exclusive of surgical fees, were examined. Eleven patients did not receive adjunctive hyperbaric oxygen therapy, whereas 10 did. The hyperbaric oxygen-treated group had an average decrease in length of stay of 14.8 days, a reduction of surgical procedures of 39%, and an average saving per case of $31,600.

Adolescent↗