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Biomedical subjects

J Sela

Publications and source records attributed to J Sela.

At least 91 records · Page 5Linked to original sources

Acinar cell hydropic degeneration induced by intraductal instillation of solutions into the parotid glands of the rats.

Saline or BSA were instilled 2, 5, 8 or 11 times at daily intervals into the parotid glands of rats, via Stensen's duct. Hydropic change of the acinar cells developed after 8 and 11 instillations. Histologically, the lesion was characterized by enlargement of the acinar cells, the cytoplasm of which contained vacuoles displacing the nuclei. In view of the negative results of various histochemical reactions the vacuolization was interpreted as hydropic in nature.

Animals↗

Cyanide removal of gold from SEM specimens.

The cyanide method is highly recommended for the purposes of the removal of gold coatings from SEM specimens prior to (a) further treatment before further SEM, and (b) staining and mounting for LM.

Animals↗

Experimental allergic sialoadenitis. X. Chronic destructive parotitis induced in immunized rats by daily intraductal challenges with antigen.

The right parotid duct of rats was instilled five to eight times with bovine serum albumin at daily intervals. Acute parotitis developed in non-sensitized animals, while chronic destructive sialoadenitis ensued in preimmunized rats. The morphological changes were reminiscent of those found in the salivary glands of patients with Sjögren's syndrome.

Acute Disease↗

The role of bone remodelling in the healing of extraction socket in rats.

Bone remodelling of post-extraction socket was studied in the past by various methods. In the present work, the process was studied with the scanning electron microscope (SEM). The SEM is essential for a three-dimensional description, particularly when hard tissues are involved. Initiation of healing on the first week was characterized by formation of delicate spongy bone. The trabeculae were distributed in a centrifugal pattern around the interradicular bone. Socket healing on the second week consisted of thickened, regularly distributed trabeculae. This was accompanied by resorption and flattening of the peri-socket sharp ridges. The third and fourth weeks were characterized by small bone marrow spaces and a gradual transformation of the trabecular bone to one of cortical-compact nature. The typical characteristics of resorbing, resting and forming surfaces were detected in all phases of socket healing. Two major patterns of ossification could be shown: Firstly, calcification of collagen bundles in osteoblastic lacunae. Concentrations of such lacunae were related to periosteal activity and were also found in areas most probably occupied previously by blood vessels. Another pattern of mineralization was in the form of globular calcospherites and is reminiscent of calcification of ground substance.

Alveolar Process↗

Experimental allergic sialoadenitis. VIII. Acute parotitis following intraductal instillation of antiserum to salivary antigens in the rat.

Antiserum to ammonium sulphate-soluble proteins of rat saliva was raised in rabbits. The introduction of this antiserum, via the excretory duct, into the parotid gland of rats induced severe acute sialoadentis. It is suggested that the inflammatory response developed as a result of formation of immune complexes, composed of acinar epithelial antigens and the antibodies introduced from without.

Animals↗

Experimental allergic sialoadenitis. IX. The regional lymph nodes after antigenic challenges of the parotid glands in rats.

The regional lymph nodes of the parotid gland of rats were examined histologically. The nodes were removed from untreated animals or rats given instillations of BSA or saline into the parotid duct. Anti-BSA antibodies were assessed in the saliva and serum. Patterns of reactivity of the lymph nodes did not correlate with the presence or absence of salivary and serum antibodies. The occurrence of antibodies in the saliva appears to reflect the activity of immunocytes residing in the parotid gland. The morphological features of the lymph nodes correspond to the overall immunological experience.

Animals↗

Tissue antigens in the saliva of rats. Demonstration of salivary gland specific and nonspecific antigens.

Antisera were raised in rabbits against the half-saturated ammonium sulfate soluble fraction of rat saliva. With these antisera, at least four antigens were demonstrated by immunodiffusion in the saliva and in a saline extract of the parotid gland; three of these antigens were also found in a saline extract of the submaxillary gland. Two of the four antigens were salivary gland specific, the other two being shared by the liver,pancreas and testis. The immunofluorescent pattern indicates that these antigens originate from the acinar epithelium of the salivary glands and probably constitute products of secretion and/or cellular turnover.

Animals↗

Antibodies in the saliva after antigenic challenge of the parotid gland of systemically sensitized rats.

Anti-BSA antibodies were detected in the parotid saliva of untreated rats. In non-sensitized animals, the parotid duct of which was repeatedly instilled with saline or BSA, the antibody titers rose significantly. Introduction of BSA into the parotid gland of sensitized animals was followed by a significant rise in salivary but not in circulating antibodies. The findings demonstrate the relative independence of the local from the systemic immune system.

Animals↗

Adaptation of silicate and Adaptic to the margins of cavities. A scanning electron microscope study.

The effects of etching of the enamel, on the adaptation of silicate and Adaptic to the cavity walls was examined with the scanning electron microscope. Replica methods were utilized to ensure that no shrinkage of the filling materials was caused by the vacuum in the instrument. It was shown that following etching of the cavity margins, Adaptic adhered to the enamel with absent, or minimal, fissure formation on one side of the filling, and some on the opposite side. The adaptation of silicate to the margins of the cavities was not improved by etching.

Composite Resins↗

Experimental allergic sialoadenitis. VII. Reactivity of the parotid gland to antigenic challenge in passively immunized rats.

Rats were passively immunized by an intraperitoneal injection of homologous anti-BSA serum and their salivary glands were challenged 20 min or 24 hours later with BSA by the intraductal route. Immune complex sialoadenitis developed only when challenge was early. It is concluded that immunoglobulins are transferred from the circulation into the salivary glands and are relatively rapidly cleared by a mechanism yet unknown, possibly by salivary flow.

Animals↗

The effect of local anesthetic solutions on the blood vessels of the rat molars.

The effect of solutions of lidocaine, with and without adrenaline, on the blood vessels of the dental pulp and periodontal ligament in rat molars was examined. The vessels were visualized by intravascular injection of a supension of carbon particles. The results indicate that the anesthetic solution alone does have a local effect on the blood vessels. Local anesthetic solutions containing adrenaline had a more marked effect; in addition, a dose-dependent systemic effect of adrenaline was noted.

Animals↗