In case of vaginal delivery herpes genitalis of pregnant women is the main reason for infection of the new-born. According to Anne Yeager, 2.4% of pregnant women with a past history if HSV infections are asymptomatically shedding herpes viruses at term without manifestation of a herpes infection of the new-born in most cases. The reasons for this could be: a sufficient antibody titre of the new-born (diaplacental transmission of maternal antibodies; term infant. According to Whitley the morbidity is about 1 to 5 infections per 10,000 deliveries; 50% of the infected infants do not show any cutaneous efflorescences and could therefore be wrong diagnosed.

Acyclovir↗

PubMedDate not supplied

Quantitative evaluation of spontaneous and radiation-induced polyploidisation processes in human and murine testes.

Flow cytometric analysis of human and murine testicular tissue was performed in order to determine whether cells exist with a DNA content differing from the expected categories 1 c, 2 c and 4 c, and to estimate the frequencies of diploid elongated spermatids. About 1.5 per cent of the murine testicular cells had an 8 c DNA content. In human testis, no 8 c cells were detected. A slight increase of 8 c cells was observed in the murine testis, following irradiation. An average of 1.8 per cent of the elongated spermatids in 10 control mice were diploid. In 12 human testicular biopsies, an average of 4.7 per cent of diploid elongated spermatids was observed among all elongated spermatids in mice. Acute or split-dose exposure, with 15 Gy roentgen rays, to spermatocytes increased the spontaneously occurring level of diploid elongated spermatids 25-fold.

Animals↗

PubMedDate not supplied

Cycling S-phase cells in animal and spontaneous tumours. I. Comparison of the BrdUrd and 3H-thymidine techniques and flow cytometry for the estimation of S-phase frequency.

Evaluation of the proliferative activities of cell populations has mainly been restricted to the use of autoradiography and flow cytometric measurements. The introduction of a new BrdUrd specific antibody makes it possible to determine exactly the DNA synthesizing cells. The BrdUrd technique is safe with respect to handling and the results are obtained within five hours. The suitability of the BrdUrd labelling procedure has been studied in different cell lines and compared with 3H-thymidine autoradiography and flow cytometry.

Animals↗