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Biomedical subjects

J Sato

Publications and source records attributed to J Sato.

At least 505 records · Page 28Linked to original sources

Mechanism of cell dissociation with trypsin and EDTA.

The mechanism of cell dissociation with trypsin and EDTA was examined. Cell dissociation was possible when trypsin and EDTA were given simultaneously, when trypsin was given after EDTA treatment, but not when trypsin was given before EDTA treatment.

Animals↗

Preparation of anti-rat albumin antiserum and its application to radioimmunoassay.

Albumin was highly purified from a commercially available rat albumin preparation (Fraction V) using disc electrophoresis. The purified albumin had the same antigenicity as Fraction V. A monospecific anti-rat albumin rabbit serum was obtained. The antiserum was used in a double antibody quantitative method for determining rat albumin.

Albumins↗

Intracranial aneurysms in geriatric patients: angiographic features and angioautotomographic analyses.

The angiographic investigation of intracranial aneurysms in geriatric patients was made by conventional cerebral angiography and angioautomography. Analyses were based on 49 aneurysms in 38 patients aged over 60 who had 24 (49.0%) large aneurysms of maximum diameter over 100 mm. This contrasted strongly with the aneurysms of patients aged under 60, only 14.4% of which were large. Cerebral angioautotomography had a particular diagnostic value for such large aneurysms, revealing not only the stalks and the whole shapes of aneurysms, but also the normal parent arteries which were hidden on conventional cerebral angiography.

Age Factors↗

A new method of magnification cerebral angioautotomography for the analysis of intracranial aneurysms.

The authors have developed a new method of magnification cerebral angioautotomography which is performed by the use of a special head-swing apparatus. From the results of experiences of 24 intracranial aneurysms in 15 patients, the method has proved useful for the intracranial aneurysms in detecting the shape and the stalk of aneurysms which were obscured or hidden on conventional magnification cerebral angiography.

Carotid Artery Diseases↗

Angiotomography of intacranial aneurysms. Analysis by a new method of angioautotomography.

The authors have developed a new method of angioautotomography by the use of a special head-swing apparatus. They have studied 70 intracranial aneurysms by this method and have found that it was more useful in clearly detecting the stalk of aneurysms than conventional angiography. The authors conclude that the method is also superior to conventional magnification angiography. Magnification angiotomography performed by this method offers more precise details.

Basilar Artery↗

Primary culture of adult rat liver cells. III. Hormonal effects on cytological and biochemical properties of primary cultured cells.

The effect of various hormones on cytological and biochemical properties of cultured hepatic cells were investigated in order to obtain long-term survival of the hepatocytes with adult liver functions in primary culture. Insulin supplementation of the culture medium enhanced the attachemnt efficiency of cells in primary culture without affecting either maintenance of morphological characters of epithelial cells or retention of liver-specific functions in cultured cells. A combination of dexamethasone and insulin was apparently effective in stimulating the formation of a monolayer of polygonal cells with granular cytoplasm and in maintenance of liver-specific functions for relatively longer periods. Supplementation with either dexamethasone or hydrocortisone alone enhanced tyrosine aminotransferase activities in cultured cells for at least 4 days postinoculation. These steroid hormones also allowed growth of small epithelial cells with clear cytoplasm and maintenance of increased albumin production for 8 days after inoculation. The roles of these hormones in the primary culture of isolated hepatic cells are discussed in the present paper.

Animals↗