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J Sapunar

Publications and source records attributed to J Sapunar.

66 records · Page 4Linked to original sources
PubMedDate not supplied

[Evaluation of ELISA-IgG test using a purified antigen in the diagnosis of human hydatidosis].

An enzyme-linked immunosorbent assay (ELISA) was developed for the detection of IgG antibodies to purified sheep hydatic cyst fluid antigen in 56 sera of confirmed cases of hydatidosis. The cut-off value was determined using serum samples from 80 healthy persons, employing two serum dilutions (1:100 and 1:500) with two and three standard deviations (SD). This assay was compared with the indirect hemagglutination test (IHAT). The sensitivity of ELISA-IgG was 94.3% for hepatic cysts and 92.9% for pulmonary cysts, whereas the values for IHAT were 77.1 and 64.3% respectively. According to Mac Nemar test, both method presented statistical significance (p < 0.05). In order to find out the specificity, additional 70 serum samples from individuals with other parasitoses, such as cysticercosis (30), trichinosis (26) and fascioliasis (14) were also tested. IHAT presented a specificity of 92.7% and for ELISA-IgG the specificity using a cut-off of mean + 3 SD was 99.3 and 100.0% with sera dilution of 1:100 and 1:500 respectively when a cut-off of mean + 2 SD was considered, we found a specificity of 91.3 and 97.3% for 1:100 and 1:500 dilutions. The use of ELISA-IgG and purified antigen in the diagnosis of human hydatidosis is discussed.

Adolescent↗
PubMedDate not supplied

[Vertebral hydatidosis with extra- and intra-rachidian hydatid ossifluent pseudoabscess].

A clinical case of a 35-year-old male with antecedents of frequent dog contacts suffering a progressive spastic paraparesis and pains of the low dorsal column is presented. Radiographies of the spinal column showed absence + of D6 right pedicle and a mass in the vicinities. A dorsal spinal column computed axial tomography revealed a retropleural mass close to D6-D7 invading the epidural space and compressing the spinal cord. An indirect hemagglutination test for hydatidosis resulted positive. Laminectomy of D5-D6-D7 and extraction of hydatic vesicles from the epidural space were performed. The patient had an excellent evolution, but four months later symptomatology reappeared with paraplegia. A new laminectomy of D8-D9, also with extraction of hydatic vesicles were carried out. As paraplegia persisted, another laminectomy of D3-D4 and D5 with extraction of additional hydatic elements was executed. Fifteen days later thoracotomy permitted to extirpate an osifluent hydatic abscess. Finally, 18 months after the onset of the illness, laminectomy of D1-D2 with extirpation of hydatic vesicles were performed. The patient was submitted to physiotherapy and rehabilitation. At present he needs a wheel chair. The characteristics of vertebral hydatidosis are commented and the frequent erroneous concepts on the parasitology and clinics of the disease are stressed.

Abscess↗
PubMedDate not supplied

[Hydatidosis of the humerus complicated with fracture, bacterial infection, fistula, and extraosseous localization].

The clinical case of an apparently healthy 63-year-old man from a rural area, with previous contact with dogs, who had a pathological fracture of the right humerus is presented. Initially he presented slight local pain, and functional discapacity. Eight months later, after a radiological study and surgery (curettage), diagnosis of hydatid disease was made. Later on, after receiving two courses with albendazole, the parent continued in similar conditions for seven years, when his situation became complicated with bacterial, fistula and extraoseous hydatidosis. The humerus was resected and a segmentary prothesis was successfully set.

Axilla↗