Biomedical subjects
Publications and source records attributed to J Sanz Esponera.
Neurofibromas are benign tumours of the nerve sheath. Histologically they vary depending on their contents of cells, myxoid stroma and collagen. A 41-year old male with radicular pain had a tumour involving the posterior chest wall. Microscopically it resulted to be a neurofibroma with abundant psammoma bodies. Although these bodies are very frequent in some neoplasias, to our knowledge they have not been described in neurofibromas to date.
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The biological significance of melanocytic lesions depends on its association with melanomas. Some melanocytic lesions are considered as precursors of melanoma while others may share histological similarities with malignant lesions. Melanomas exhibit clinical, epidemiological and histological heterogeneity. Molecular and cytogenetic studies may supply additional information to supplement the histopathological evaluation. Several genes have been linked to melanomas: CMM1 on chromosome 1p36 and CMM2 on 9p21, and other chromosomal regions where tumor suppressor genes are located as p16 (9p21) and p53 (17p13). We analyzed different melanocytic lesions to determine LOH at 9p21 and 17p13 and to assess clonality by the HUMARA technique. All the malignant melanomas were monoclonal and all the benignant lesions we analyzed were policlonal in our series including deep penetrating nevi and Spitz Nevi. LOH at 9p21 was determined in 60% melanomas, 50% Spitz and 0% intradermal nevi. In conclusion, genotypic changes may supplement the phenotypic or morphological evaluation of melanocytic lesions.
Lung cancer is the leading of death in both, women and men in the United States and many European countries. molecular cytogenetic and LOH analyses and LOH analyses of non-small cell lung cancer have shown somatic genetic alterations in a variety of chromosomes such as 1p, 3p, 5q, 8p, 9p, 11p, 11q and 17p. Putative tumor suppressor genes have been located in these lci. Allelic loss at 3p21, 9p21 and 5q21 has been also reported in premalignant epithelial lesions of the bronchus. We investigated 33 cases of NSCLC for LOH at 3p, 5q, 9p and 17p: 22 squamous cell and 11 adeno carcinomas. Normal lymphocytes, tumor cells and normal bronquial cells adjacent to the tumor were microdissected from paraffin embedded tissues. PCR amplification was performed utilizing the specific markers D5S299, D5S346, D3S1300, D9S157, D9S171 and D17S799. Our results show that within the NSCLC tumor cells, LOH was more frequently found at the 5q21 locus (75% of the informative cases), the 3p21 locus (48%) and 17p. Within the normal bronquial cells, LOH was found in 33% of the cases at 5q21 and 33% of the cases at 3p21. In conclusion our results show that LOH can be assessed in normal bronquial mucosae adjacent to NSCLC. We suggest that LOH at these loci may be present before the onset of the malignant growth.
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A 27-year-old woman with a Malignant Triton Tumor (MTT), or malignant schwannoma with rhabdomyoblastic differentiation, located in the parotid cell and infiltrating the nasal sinuses and the left orbit is described. The initially resected tumor showed three recurrences within a 2 years follow-up period. During successive recurrences an increase in cellular density, number of mitoses and necrosis was noticed. Immunohistochemical analysis showed that the tumor was composed of a mixed population of cells. Some of them showed positivity for actin, desmin and myoglobin, while others were positive for S-100 protein, glial fibrillary acid protein, and IV-collagen. Cytokeratin stainings were negative. Up to now, 8 benign triton tumors and another 45 cases of MTT have been described. None of them was primarily located in the parotid gland, and infiltration to the orbital cavity has not been previously described.
Primary malignant lymphoma of the bladder is a rare tumour. In a recent literature search only 70 cases have been found since 1885. Most of these tumours were low grade B-cell non Hodgkin's lymphomas and only 20% were high grade neoplasms. This tumour usually appears in women between 20 and 85 years (median 64 yr.) of age. 20% of them have antecedents of chronic cystitic. Some authors have considered that some of the primary malignant lymphomas of the bladder arise from the mucosa-associated lymphoid tissue (MALT), therefore acting like MALT lymphomas which affect the gastrointestinal tract, the salivary glands or the thyroid. We report three additional cases of primary malignant lymphoma of the bladder, two of high grade, and discuss the histological criteria that support their MALT nature.
Metastases to the tonsils are extremely infrequent. Less than 70 cases have been reported in literature since 1858. The commonest sources of tonsillar metastases are malignant melanomas and carcinomas of the breast and the lungs. We report about two new cases of tonsillar metastases, one of which developed from a malignant melanoma, the other one from a hepatocellular carcinoma. We have not found any reports on tonsillar metastases stemming from hepatocellular carcinomas in literature and, moreover, in our case, this was the clinical presentation of the primary tumor.
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Benign tumors of the urinary tract are rarer than transitional cell carcinomas. Among the benign tumors we have those of epithelial origin (papillomas or low grade transitional cell carcinomas) and those of mesodermal origin (fibrous and angiomatous polyps, hemangiomas and lymphangiomas and neurofibromas), together with a group of miscellaneous lesions including endometriosis, amyloidosis and granulomas. The fibroepithelial polyps of the urinary tract represent between 2-6% of all the benign tumors. This rare tumor is located more frequently in the ureter and only in 11 cases reported since 1929 the location was the renal pelvis. Between 1976 and 1994 we have seen three cases of fibroepithelial polyps of the urinary tract, two of them affecting the renal pelvis and one of them affecting the ureter. We report the cases located in the pelvis and make a review of the cases reported in this location in the last years and discuss the clinical, radiological and cytological criteria that can lead to a preoperative diagnosis of this entity avoiding an unnecessary nephroureterectomy.
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The authors describe a Juxtaglomerular Cell Tumor (JGCT) in a hypertensive young man whose symptomatology disappeared when the JGCT was removed. The clinical diagnosis was made before surgery by selective determination of plasma renin activity level in both renal veins and in the draining vein of the tumor. This neoplasm is benign and usually occurs in young patients. The morphological, immunocytochemical and ultrastructural features are described. Other renin-secreting tumors and the theories about histogenesis of JGCT are discussed.
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The characteristics of brain tumors in vitro are analyzed in relation to their morphology, ultrastructure, immunocytochemistry and cell growth, showing that it is possible to identify the cell origin and to know several biological characteristics of the primitive tumor.