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Biomedical subjects

J Santelli

Publications and source records attributed to J Santelli.

26 records · Page 2Linked to original sources
PubMed1985

Pursuing and distancing: the construct and its measurement.

Although the concept of interpersonal pursuing and distancing has been introduced and used clinically, the lack of a reliable and valid measure has deterred its more formal investigation and relevance to personality theory. The Pursuing-Distancing (P-D) Scale, a 92-item measure of the behavioral expression of these characteristics, was developed. Evidence for internal consistency as well as internal support of construct validity is presented and discussed. An 80-item revised scale is available from the authors and is currently being evaluated in a variety of external validation settings.

Journal Article↗
PubMed1980

A simple method for maintaining large, aging populations of Caenorhabditis elegans.

This paper describes a technique capable of establishing and maintaining large, age-synchronous populations of the nematode Caenorhabditis elegans. The technique has three essential components: a rich chemical medium; a method for producing and harvesting mass quantities of eggs; and 5-fluorodeoxyuridine (FUdR), an inhibitor of DNA synthesis. A culture of worms is filtered through glass wool or a wire screen to isolate young larvae. Eggs laid by these worms after they mature are collected over a period of 4-6 hours and allowed to hatch. A low level of FUdR (25 microM) is added just before the larvae reach maturity. This timing is important to avoid developmental abnormalities. The adults lay eggs in the presence of FUdR but the eggs do not hatch, which maintains the synchrony of the culture. Many aging characteristics appear to be similar in treated and untreated worms, such as the time of cessation of egg production, the appearance of visible and behavioral age-related changes, and the mean lifespan. This system thus seems suitable for large-scale biochemical analysis of certain aspects of aging in C. elegans.

Aging↗
PubMed1979

Synchronous growth and aging of Caenorhabditis elegans in the presence of fluorodeoxyuridine.

Fluorodeoxyuridine (FUdR), an inhibitor of DNA synthesis, was examined for its ability to prevent a synchronous population of C. elegans from reproducing without otherwise interfering with the organism's post-maturational development and aging. When a synchronized population was exposed to 400 micrometer FUdR just as the population reached sexual maturity, the FUdR induced complete sterility within five hours by preventing eggs from hatching. Any larvae that hatched from eggs made before the FUdR was added remained small in the presence of FUdR and were easily removed by filtration or sedimentation. FUdR-sterilized adults showed no morphological abnormalities. Age-associated changes seen in controls also occurred in FUdR-treated worms, including atrophy of the gonads, increased pigmentation, sluggishness and increased transparency. Life span was not shortened by FUdR treatment. Our observations suggest that treatment with FUdR under carefully controlled conditions is a reasonable way to maintain synchronously aging populations of C. elegans.

Aging↗