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J STOKES

Publications and source records attributed to J STOKES.

102 records · Page 6Linked to original sources
PubMed1950

Studies on the agent of infectious hepatitis; propagation of the agent in tissue culture and in the embryonated hen's egg.

Two viral agents have been procured from patients with infectious hepatitisin two widely separated outbreaks of the disease by transfer of acute stage serum and stool filtrates to and passage in tissue cultures of rabbit liver cells in roller tubes and minced chick embryos in Simms-Sanders medium followed by passage in the amniotic cavity of the chick. Cultures of both agents, designated the Akiba and NL strains of virus, induced mild hepatitis without jaundice in the majority of volunteers tested after an incubation period of from 9 to 38 days. Although these agents have not been identified definitely as the virus of infectious hepatitis, the available evidence, as discussed, is compatible with the suggestion that such they are.

Animals↗
PubMed1950

Studies on the agent of infectious hepatitis; the disease produced in human volunteers by the agent cultivated in tissue culture or embryonated hen's eggs.

The successful cultivation of the virus of infectious hepatitis in chick embryo tissue culture and in the amniotic cavity of the embryonated hen's egg is supported by a comparison of the disease induced in volunteers by the cultivated virus with hepatitis without jaundice resulting from experimental infection with natural infectious hepatitis virus. Both types of viral preparations produced illnesses in comparable percentages of volunteers (83 and 75 per cent, respectively) after similar average periods of incubation (24.4 and 23.4 days, respectively) and of similar average duration (28.3 and 27.6 days, respectively). The disease could be divided in both groups of patients into a primary stage, followed after a short interval of relative well being by the secondary stage. The illnesses in both instances were characterized by anorexia, nausea, vomiting, enlarged, tender livers and abnormal liver function tests, and frequently temperature elevations. They differed in that jaundice was observed in 31 per cent of the cases resulting from infection with natural virus but not in any patients infected with the cultivated virus.

Animals↗