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Biomedical subjects

J S Fine

Publications and source records attributed to J S Fine.

42 records · Page 3Linked to original sources
PubMed1984

Creatine kinase isoenzymes in human cerebrospinal fluid and brain.

Extracts of normal brains obtained at autopsy and cerebrospinal fluid (CSF) from patients with global brain ischemia were analyzed for creatine kinase (CK; EC 2.7.3.2) isoenzymes. We used both qualitative and quantitative assays (electrophoresis and immunoinhibition). Brain extracts contained CK-BB isoenzyme and mitochondrial CK. In 54 CSF samples free of blood contamination and with total activities ranging from 7 to 2010 U/L (mean 202 U/L), virtually all of the CK activity was due to CK-BB, and none to CK-MM or CK-MB. We conclude that brain contains CK-BB and mitochondrial CK, but lacks CK-MM and CK-MB. After cardiac arrest, CK-BB is released into the CSF. Any CK-MM in the CSF is probably from blood contamination, in which case immunoinhibition with anti-CK-M antibodies accurately quantifies CK-BB.

Acetates↗
PubMed1982

Growth of high aflatoxin B2 mutants on defined and complex media and with ethoxyquin.

The major naturally occurring aflatoxins are B1, B2, G1, and G2. A precursor-product relationship between B1 and the other toxins has been assumed by many workers, but experimental data are conflicting. Four independent reports, of mutants which produce aflatoxin B2, provide genetic evidence supporting an alternate origin for B2. Five high aflatoxin B2 mutants were obtained and grown in defined and complex liquid media and on a solid complex medium in order to determine the stability of the high B2 phenotype on different substrates and to define optimal levels of production. All the high B2 strains tested produced some aflatoxin yields were quite variable. The highest production was on complex liquid medium containing yeast extract. It was not possible to confirm reports that the antioxidant ethoxyquin was an inhibitor of G aflatoxins.

Aflatoxins↗
PubMed1975

A more sensitive automated method for determination of ornithine carbamoyltransferase activity in human serum.

Ornithine carbamoyltransferase (EC 2.1.3.3) activity is a sensitive, specific indicator of hepatocellular injury. This paper describes development of an improved automated procedure for measurement of this activity. Triethanolamine-ethylenediaminetetraacetate is used as a buffer, and activity is determined by measuring the concentration of the product, citrulline. Kinetic studies have been performed to determine optimal pH and L-ornithine and carbamoyl phosphate concentrations. Recovery of citrulline was studied. The upper limit of normal obtained in a study of 106 blood-bank donors was 6 U/liter. The automated procedure developed as a result of these studies, in which optimal assay conditions are used, produces a threefold increase in sensitivity and permits use of a sample volume of 1 ml.

Adult↗
PubMedDate not supplied

Computer programs that teach the interpretation of image-based laboratory tests.

OBJECTIVE: To review the effort of the University of Washington (UW) Department of Laboratory Medicine to develop and use personal computer programs to teach the interpretation of image-based clinical laboratory tests to medical technologists and other health care workers. DATA SOURCES: Professional journals and books; Software owned by and licensed by the University of Washington. STUDY SELECTION: Not applicable. DATA EXTRACTION: Not applicable. DATA SYNTHESIS: We have been developing interactive personal computer (PC) programs for teaching image-based laboratory tests to medical technologists and other health care workers. The programs, called "Laboratory Tutors," are useful for teaching microscope-based tests and tests based on electrophoresis. Our programs include ANA-Tutor, which teaches the immunofluorescence assay for anti-nuclear antibodies; Gram Stain-Tutor, which teaches the direct Gram stain; Electrophoresis-Tutor, which teaches the interpretation of agarose gel protein electrophoretic patterns; Urinalysis-Tutor, which teaches the microscopic examination of urine sediment; in addition to other programs. The tutorials are all based on high-quality digital images that were acquired and processed using digital imaging systems. They require minimal computer literacy and have a number of advantages over standard approaches to teaching image-based laboratory tests. The computer tutorials are used in UW's medical technology and medical school curriculum, where they are used as supplements to traditional instruction. CONCLUSION: Laboratory tutors are computer programs that use high resolution digital images to teach the interpretation of image-based laboratory tests. We plan to continue to develop these programs, study their educational effectiveness, and update them periodically.

Clinical Laboratory Information Systems↗
PubMedDate not supplied

The practice of adolescent medicine in staff model HMOs.

Adolescent medicine is a medical specialty that may be represented in a prepaid group practice, but has generally not been perceived as necessary. A survey of The HMO Group shows considerable variation in staffing and the centralization of adolescent health care. An adolescent medicine practice may be rapidly created and promoted through notification of patients and professionals. Adolescent medicine providers can attend to age-appropriate educational and office setting needs of their patients, and study quality assurance of adolescent care by others. The HMO may benefit from improved age-specific care and increased physician and patient satisfaction. Problems with coverage and staffing may occur. HMOs should consider using one adolescent medicine specialist for each five to six pediatricians.

Adolescent↗