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Biomedical subjects

J Roche

Publications and source records attributed to J Roche.

At least 145 records · Page 8Linked to original sources
PubMed1980

Adrenocorticotropin analogs and glucocorticoids in the hypophysectomized rat. I. Effects on liver polyribosomes and rough endoplasmic reticulum.

Hypophysectomy decreased polyribosome aggregation and activity in rat liver. Polyribosome aggregation was measured by sucrose density gradient analysis and polyribosome activity was determined by the incorporation of labeled amino acids into protein in a cell-free system using pH 5 enzymes from sham-operated rats. Hypophysectomy also totally disorganized the rough endoplasmic reticulum of hepatocytes, as shown by electron micrographs. The sc administration of the ACTH fragments, Hoe 433 (corticotropic) and Org 2766 (noncorticotropic), and of glucocorticoids (dexamethasone or corticosterone) to hypophysectomized rats restored these parameters to the levels recorded in sham-operated rats in a dose-dependent fashion 5 h after injection; the effects were maintained until at least 24 h. The sc administration of the noncorticotropic but metabolically less stable fragment, ACTH-(1-10), had only a partial effect. The potency of a noncorticotropic ACTH fragment (Org 2766) in hypophysectomized rats suggests that ACTH may act on liver protein synthesis without the intervention of glucocorticoids or pituitary hormones.

Adrenocorticotropic Hormone↗
PubMed1978

Fever tester.

Explore the source record for details and available documents.

Fever↗
PubMed1978

The relationship between guanosine tetraphosphate, polysomes and RNA synthesis in amino acid starved Escherichia coli.

A relA+ strain of E. coli with four amino acid requirements was starved separately for each amino acid, after which the levels of polysomes, guanosine-5'-diphosphate-3'-diphosphate and the residual net synthesis of RNA were determined. The polysome level and guanosine-5'-diphosphate-3'-diphosphate production were coordinately affected by starvation for the different amino acids, whereas no correlation was found between these two parameters and residual RNA synthesis. The main conclusion stemming from these results is that guanosine-5'-diphosphate-3'-diphosphate cannot act as the sole effector molecule in stringent control of RNA synthesis.

Amino Acids↗
PubMed1978

Differential effect of amino acid starvation on polysome decay in Escherichia coli.

In a relA+ strain of E. coli starved separately for each of four required amino acids, the intracellular concentration of polysomes decreases as a function of time in all cases: very rapidly in the absence of arginine or leucine, slowly in the absence of threonine or histidine. In a starved isogenic relA strain, the polysome level is either totally stable or else drops slowly. The decrease in the level, when it occurs, does not significantly affect the polysome size distribution. Models for polysome metabolism in amino acid starved cells are discussed.

Amino Acids↗
PubMed1978

Thyroglobulin biosynthesis in a larval (ammocoete) and adult freshwater lamprey (Lampetra planeri Bl.).

1. The biosynthesis of 18-19S thyroglobulin has been studied in a larval and adult freshwater lamprey (Lampetra planeri Bl.). 2. In vivo and in vitro experiments have been performed by injecting into the coelomic cavity or by incubating branchial region labeled constituents of Tg of higher vertebrates (125I, [3H]leucine and various [3H]carbohydrates). 3. Larvae (ammocoetes) and adults incorporate all labels into thyroglobulin (18-19S Tg), containing a small proportion of labeled T3 and T4, as identified by paper chromatography, and very minute amounts of stable iodine. 4. In adults, the biosynthesis of 18-19S Tg proceeds much more rapidly and the labels are incorporated in higher percentage than in larvae. 5. The demonstration of the biosynthesis of the specific thyroid protein, 18-19S Tg, in larvae indicates that the biochemical mechanism of hormonogenesis is present in larval endostyle before the morphological differentiation of thyroid cells and follicles occurring during metamorphosis. 6. Some 18-19S Tg is apparently stored in the endostyle.

Aging↗
PubMed1978

Angiodysplasia of the small bowel: a method of intraoperative identification.

Two cases of recurrent bleeding from the gastro-intestinal tract are described. The initial diagnosis and the identification of the lesions at laparotomy were difficult problems. In both cases the bleeding was due to a vascular lesion of the small bowel, referred to as angiodysplasia. The lesions were detected before operation by selective angiography and identified at laparotomy by a preoperative and an intraoperative injection of a vital stain through the catheter into the feeding vessels.

Aged↗
PubMed1978

Increase in amylase clearance in relation to creatinin clearance during acute pancreatitis.

Renal amylase clearance in relation to creatinin clearance (Cam/Ccr) was determined for 20 patients suffering from acute pancreatitis (AP). For each of them, the diagnosis rested upon operative or laparoscopic observation of characteristic anatomical or histological lesions. The average clearance relationship in these patients reached 16 +/- SEM 3.8%. These results were significantly higher than those obtained from 132 patients who had been hospitalized for other medical or surgical diseases, as well as from 51 test patients (p less than 0.001). The authors stressed the diagnostic importance of this test, in particular in the presence of a normal amylasemia level.

Acute Disease↗