Biomedical subjects

J Repka

Publications and source records attributed to J Repka.

9 recordsLinked to original sources

PubMed2006

Neutrophil oxidative metabolism in diabetic patients undergoing pancreas transplantation.

UNLABELLED: A pancreas transplantation is the only therapy capable of returning a constant, physiological euglycemic state to diabetic patients. Considering the clinical controversies in the study of infection in diabetes and the recognized effect of insulin on the oxidative metabolism of glucose in phagocytes, the present study sought to evaluate the formation of intraphagocytic oxygen-free radicals in diabetic patients undergoing simultaneous pancreas kidney transplantation (SPK). METHODS: Twenty-five diabetic patients undergoing SPK were compared with 25 normal individuals. Evaluation of the oxidative metabolism of leukocytes was performed using the NBT test. RESULTS: The abnormality in the pretransplant counts (19.32%-28.2%) reached normal levels at 48 hours after transplantation (45.11%-76.25%) and was maintained to the 5th day (46.28%-76.20%). CONCLUSION: An SPK in a diabetic patient normalized the formation of intraphagocytic oxygen-free radicals.

Adult↗

PubMed2006

Pancreatic islet cells antibodies in diabetic patients submitted to pancreas transplantation.

Several pieces of evidence suggest an autoimmune etiology of diabetes mellitus type 1. To trace patients who are susceptible to the disease, we utilized islet cells antibodies (ICAs). The aim of this study was to evaluate the presence of ICAs among diabetic patients undergoing simultaneous transplantation of the pancreas and kidney (SPK). Twenty-six diabetic patients received an SPK, 12 of whom were included in this analysis. The indirect immunofluorescence method was utilized for quantitation of ICAs. The types of ICAs were no different following transplantation of the pancreas. The serum levels of pre-existent ICAS in diabetic patients undergoing SPK with immunosuppression were not reduced, and they did not interfere with the function of the implanted pancreas over a period of 60 days.

Adult↗

PubMed2001

A bayesian framework for parentage analysis: the value of genetic and other biological data.

We develop fractional allocation models and confidence statistics for parentage analysis in mating systems. The models can be used, for example, to estimate the paternities of candidate males when the genetic mother is known or to calculate the parentage of candidate parent pairs when neither is known. The models do not require two implicit assumptions made by previous models, assumptions that are potentially erroneous. First, we provide formulas to calculate the expected parentage, as opposed to using a maximum likelihood algorithm to calculate the most likely parentage. The expected parentage is superior as it does not assume a symmetrical probability distribution of parentage and therefore, unlike the most likely parentage, will be unbiased. Second, we provide a mathematical framework for incorporating additional biological data to estimate the prior probability distribution of parentage. This additional biological data might include behavioral observations during mating or morphological measurements known to correlate with parentage. The value of multiple sources of information is increased accuracy of the estimates. We show that when the prior probability of parentage is known, and the expected parentage is calculated, fractional allocation provides unbiased estimates of the variance in reproductive success, thereby correcting a problem that has previously plagued parentage analyses. We also develop formulas to calculate the confidence interval in the parentage estimates, thus enabling the assessment of precision. These confidence statistics have not previously been available for fractional models. We demonstrate our models with several biological examples based on data from two fish species that we study, coho salmon (Oncorhychus kisutch) and bluegill sunfish (Lepomis macrochirus). In coho, multiple males compete to fertilize a single female's eggs. We show how behavioral observations taken during spawning can be combined with genetic data to provide an accurate calculation of each male's paternity. In bluegill, multiple males and multiple females may mate in a single nest. For a nest, we calculate the fertilization success and the 95% confidence interval of each candidate parent pair.

Animals↗

PubMed2000

Statistical confidence in parentage analysis with incomplete sampling: how many loci and offspring are needed?

We have recently presented models to estimate parentage in breeding systems with multiple mating and incomplete sampling of the candidate parents. Here we provide formulas to calculate the statistical confidence and the optimal trade-off between the number of loci and offspring. These calculations allow an understanding of the statistical significance of the parentage estimates as well as the appropriate sampling regime required to obtain a desired level of confidence. We show that the trade-off generally depends on the parentage of the putative parents. When parentage is low, sampling effort should concentrate on increasing the number of loci. Otherwise, there are similar benefits from increasing the number of loci or offspring. We demonstrate these methods using genetic data from a nest of the bluegill sunfish (Lepomis macrochirus).

Animals↗

PubMed2000

Parentage analysis with incomplete sampling of candidate parents and offspring.

Many breeding systems include 'multiple mating' in which males or females mate with multiple partners. We identify two forms of multiple mating: 'single-sex', where the next-generation individuals (NGIs) are the product of multiple mating by one sex; and 'two-sex', where the NGIs are the product of multiple mating by both sexes. For both mating systems we develop models that estimate the proportion of NGIs that is fathered (paternity) or mothered (maternity) by the putative parents. The models only require genetic data from the parent or parents in question and the sample of NGIs, as well as an estimate of population allele frequencies. The models provide unbiased estimates, can accommodate loci with many alleles and are robust to violations of their assumptions. They allow researchers to address intractable problems such as the parentage of seeds found on the ground, juvenile fish in a stream, and nestlings in a communal breeding bird. We demonstrate the models using genetic data from a nest of the bluegill sunfish Lepomis macrochirus, where the NGIs may be from multiple females that have spawned with multiple males from different life histories (cuckolder and parental).

Alleles↗

PubMed1998

Stability with Inheritance in the Conditional Strategy.

The conditional strategy is a theoretical framework that explains the existence within populations of individuals that express alternative behavioral, physical or life history tactics (phenotypes). An example is fighters and sneakers in many animal mating systems. In the conditional strategy the alternative tactics are chosen by individuals based on their state, for example large or small bodied. Since state is often heritable, due for example to additive genetic variance, the alternative tactics may also have inheritance. As the tactics do not have equal fitnesses, it is generally believed that any such inheritance would prevent the evolutionary stability of the conditional strategy. However, in previous work we introduced an Inheritance Theorem and were able to prove that a conditional strategy with tactic inheritances can have a unique equilibrium proportion of the tactics. We now prove a second property of our Inheritance Theorem, namely the stability of the equilibrium. This means that if the tactics are perturbed from their equilibrium proportions, they will return across generations to their equilibrium proportions. An example is provided in mites. We have therefore established an Inheritance Theorem which includes both the existence of an equilibrium and its stability for alternative tactics in a conditional strategy.Copyright 1998 Academic Press Limited

Journal Article↗

PubMed1998

Using a nonrecursive formula to determine cladogram probabilities.

Three properties of bifurcating branching diagrams that are used for representing a specific number of taxa are (1) the number of possible arrangements, (2) the number of possible topologies, and (3) the probabilities of formation according to particular models of cladogenesis. Of these, the probabilities have received the least attention in the literature. Indeed, many biologists would be astonished by the observation that the probability of a commonly cited cladogram containing 35 phyla of the animal kingdom is < 0.0072% of the value of the average probability taken over all possible cladograms! We reviewed works on cladogram arrangements and topologies and developed a computer-generated table of enumerations that extends and corrects such tables in the literature. We also developed a nonrecursive formula for the determination of cladogram probabilities. This formula facilitates calculation and thereby should promote use of cladogram probabilities, which might provide more accurate null hypotheses for tests of cladogenic events than do considerations of cladogram arrangements or topologies.

Animals↗

PubMed1995

The evolutionarily stable strategy under individual condition and tactic frequency.

A proof is presented to show that, when fitnesses from alternative tactics within a population depend on both their frequency and the phenotypic condition of individuals, there will be a unique ESS switchpoint s* that determines both the condition at which an individual will switch between tactics and the resulting frequency of the tactics in the population. For an individual at the ESS s*, the fitnesses of the alternative tactics will be equal. When fitness is averaged over the population, however, the average fitnesses of the alternative tactics will not be equal.

Animals↗

PubMedDate not supplied

A Bayesian model for assessing the frequency of multiple mating in nature.

Many breeding systems have multiple mating, in which males or females mate with multiple partners. With the advent of molecular markers, it is now possible to detect multiple mating in nature. However, no model yet exists to effectively assess the frequency of multiple mating (f(mm))--the proportion of broods with at least two males (or females) genetically contributing--from limited genetic data. We present a single-sex model based on Bayes' rule that incorporates the numbers of loci, alleles, offspring, and genetic parents. Two genetic criteria for calculating f(mm) are considered: the proportion of broods with three or more paternal (or maternal) alleles at any one locus and the total number of haplotypes observed in each brood. The former criterion provides the most precise estimates of f(mm). The model enables the calculation of confidence intervals and allows mutations (or typing errors) to be incorporated into the calculation. Failure to account for mutations can result in overestimates of f(mm). The model can also utilize other biological data, such as behavioral observations during mating, thereby increasing the accuracy of the calculation as compared to previous models. For example, when two sires contribute equally to multiply mated broods, only three loci with five equally common alleles are required to provide estimates of f(mm) with high precision. We demonstrate the model with an example addressing the frequency of multiple paternity in small versus large clutches of the endangered Kemp's Ridley sea turtle (Lepidochelys kempi) and show that females that lay large clutches are more likely to have multiply mated.

Animals↗