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Biomedical subjects

J Raszyk

Publications and source records attributed to J Raszyk.

At least 37 records · Page 2Linked to original sources
PubMed1986

[The clinical course and differential diagnosis of papular dermatitis in fattened pigs].

Since December 1981, dermatitis of unknown etiology in form of papular lesions has appeared in fattened pigs in some large herds in the Czech Socialist Republic. Clinical investigations of diseased pigs were performed in a large fattening house D. and in a large production herd of pigs M. in the region with frequent mines (lignite extraction). The diameter of the papules is mostly 3-5 mm, they are not painful when touched, are straggled irregularly, their large numbers are on the upper surface of the body. Papular dermatitis has a chronic course, affecting the gilts and castrated boars from the body weight of 30 kg, irrespective of their nutritional status. The overall health condition of pigs is not impaired and the disease does not cause itch. In the infected herds, there are usually fewer than 30% of pigs suffering from the disease. The incidence of the disease is highest in winter months. It is necessary to distinguish by differential diagnostics: swine pox, parakeratosis of swine, lesions of impetigo contagiosa suum, pustular dermatitis and scab of swine, from rarely occurring skin diseases of swine hypotrichosis cystica suis and demodicosis of swine. Neither did preventive nor therapeutic measures reduce expressively the incidence of skin lesions. We hope that the complex investigation of the internal environment of the infected pigs could help to reveal the pigs sensitive to the infection by this disease.

Animals↗
PubMed1986

[Biological, chemical and pathohistologic study of the skin in papular dermatitis in fattened pigs].

We examined the skin samples from 23 fattened pigs with papular dermatitis (PD) and from 17 pigs showing no skin lesions; the live weight of the pigs was from 95 to 105 kg and the pigs came from a large fattening house D that is located in the region where the mines are frequent (lignite extraction). It was only in 26% of pigs with PD where the common ectoparasites Haematopinus suis and Sarcoptes scabiei var. suis were found out. The skin lesions were not fluorescent in the light of Wood's lamp. The fungi Aspergillus flavus, A. fumigatus, A. tereus, Chrysosporium, Mucor sp., Scopulariopsis sp. were sporadically isolated from the skin lesions in 39% of pigs with PD. In 65% of pigs with PD Staphylococcus aureus was isolated from the skin lesions, which was not found in pigs without dermal lesions. Staphylococcus epidermidis was isolated in 87% of pigs with PD and 58% of pigs without any skin changes. The skin lesions were virus-free. Increased concentrations of cadmium and mercury were observed in the skin of pigs with PD and in control pigs. The concentration of aflatoxin B1 in the skin of the two groups of pigs did not exceed the level of 5 micrograms X kg-1, in PD pigs it was, however, twice higher than in the clinically healthy pigs. The residues of chlorinated hydrocarbons in the subcutaneous fat were not higher than the certified hygienic levels. Both epidermis and corium are affected by inflammatory lesions. At the beginning, neutrophil infiltration with necrotization of epithelial cells prevails. It is followed by capillary proliferation with angioblast and fibroblast multiplication and straggled lymphoid infiltration. It is necessary to distinguish by differential diagnostics: swine pox, parakeratosis of swine, lesions of impetigo contagiosa suum, pustular dermatitis and scab of swine, and from rarely occurring skin diseases of swine hypotrichosis cystica suis and demodicosis of swine. No conclusions can be drawn explicitly on the etiology and pathogenesis of papular dermatitis in fattened pigs. We must study the potential interactions of negative environmental factors with regulating homeostatic and adaptation mechanisms in fattened pigs.

Animals↗
PubMed1986

[Biochemical, hematologic, immunologic and cytogenetic study of papillary dermatitis in fattened pigs].

Examinations were performed of 31 fattened pigs suffering from papular dermatitis (PD) and 33 fattened pigs without dermal changes (C) coming from the D. large pig-fattening farm and the production breeding herd of pigs. The weight of the pigs was from 95 to 105 kg. Both farms are sited in a mining area (intensive lignite extraction). The PD pigs, compared with the controls (C), had less total protein, cholesterol and calcium in their blood serum and increased concentrations of thyroxine (T4), non-esterified fatty acids (NEFA), vitamins A and E, inorganic phosphorus and potassium, and the higher alanine aminotransferase (ALT) activity. The PD pigs also had an increased leucocyte count in blood. In their peripheral lymphocytes the PD pigs had a significantly higher number of aberrant cells, 3.9 times higher number of chromosome breaks, slightly increased rate of sister chromatid exchanges, but half the number of chromosome exchanges of the C pigs. No differences between PD pigs and the controls were recorded in the response of the T-lymphocytes of peripheral blood to non-specific phytohaemagglutinin stimulation; neither were there any substantial differences in the concentration of serum immunoglobulins of the IgG, IgM and IgA classes. The PD and C pigs of both herds (the D. and M. farms) had low concentrations of all three serum immunoglobulins. The higher occurrence of PD in the D. herd, compared with the M. farm, is associated with a greater reduction of serum immunoglobulins (IgM by 38%, IgA by 25%, IgG by 15%).

Animals↗
PubMed1986

[Trends in the quantity of foreign substances in feed mixtures and in pigsty dust at large pig farms].

In the period from January 1983 to December 1985 we examined thirty-five samples of commercial feed mixtures for pigs and thirty samples of pigsty dust deposition from large pig-houses in a region with extensive mining (lignite extraction) situated in the Hodonín district. In comparison with the contents of contaminants in the feed mixtures over the period from January 1983 (1/83) to June 1984 (6/84), the zinc and lindane concentrations increased to 213.3% (P less than 0.01) and 133.3%, respectively, from July 1984 (7/84) to December 1985 (12/85). The concentrations of copper, manganese, lead, cadmium, mercury (P less than 0.05), DDT (P less than 0.01) and aflatoxin B1 in the feed mixtures decreased. The highest permissible amount of contaminants in the feed mixtures was exceeded in the 1/83-6/84 period in cadmium (limit value 0.3 mg per kg) and mercury (limit value 0.1 mg per kg), in the 7/84-12/85 period only in zinc (limit value 250 mg per kg). In comparison with the values of contaminants in pigsty dust deposition recorded from January 1983 to June 1984, the lead concentration increased to 555.5% in the 7/84-12/85 period, lindane concentration rose to 569.0% (P less than 0.01), zinc concentration to 220.5% (P less than 0.01), the copper, manganese, cadmium, mercury concentrations (P less than 0.05) decreased, and the concentrations of DDT, aflatoxin B1 and polychlorinated biphenyls also dropped. It is desirable to evaluate in regular intervals the spectrum of contaminants both in feed mixtures and in pigsty dust deposition and to monitor other potential sources of contaminants in the pig-houses.

Aflatoxins↗
PubMed1986

[Influence of the migration of Ascaris suum larvae on blood parameters in the pig].

Biochemical and hematological blood parameters were investigated in pigs experimentally infected with Ascaris suum. The most marked changes were recorded on day 5-8 post inoculation, during fever. In this period there was a marked decrease of concentrations of albumin, iron, thyroxin, A and E vitamins in blood serum, while compared with the control group the contents of A vitamin in liver did not change. Further, the concentrations of nonesterified fatty acid increased, as well as those of inorganic phosphorus and 11-hydroxycorticosteroids. Till day 13 PI the decrease of albumin and iron concentrations intensified and a recession of cholesterol serum binding capacity for iron was also recorded. Our results suggest that the acute phase of pig ascariasis is accompanied by changes in the host metabolism, generally corresponding to findings in fever states of different aetiology.

11-Hydroxycorticosteroids↗
PubMed1985

[Digestibility of nutrients in a feed mixture in certain swine helminthiases].

The average digestibility coefficients of feed mixture nutrients in pigs infected medium severely to severely by the helminths Ascaris suum and Oesophagostomum dentatum were lower in comparison with the post-dehelminthization condition (dry matter by 3.1%, crude protein by 6.7%, fat by 8.9%, nitrogen-free extracts and fibre by 2.2%, organic matter by 3.1%). The content of total digestible nutrients made 679 g in infected pigs and 708 g in dehelminthized pigs. In the period following the experimental infection of pigs by the helminths Ascaris suum, characterized by the formation of antibodies and adaptation after heavy injury of the organism, the digestibility coefficients of feed mixture nutrients were in comparison with the state prior to infection influenced positively. In two metabolism trials, on the 9th to 13th day after infection the digestibility coefficients increased as follows: of dry matter by 4.8% and 3.6%, crude protein by 8.2 and 4.2%, nitrogen-free extracts and fibre by 2.0 and 3.5% and organic matter by 3.0 and 3.3%. The decrease in fat digestibility coefficients was observed in both cases, by 12.4% and 9.9%. Prior to infection the content of total digestible nutrients was 712 g and 682 g, after infection 731 g and 707 g.

Animal Feed↗
PubMed1984

Biochemical changes in sows after eleven or more farrowings.

The animals examined were 30 sows after eleven or more farrowings (pluriparous sows) and 26 primiparous sows. The pluriparous animals were 75.1 months old on the average and had farrowed an average of 13.23 litters. The mean age of the primiparous sows was 12.8 months. The blood sera of pluriparous sows had higher concentrations of total protein and urea and lower levels of 11-hydroxycorticosteroids (11-OHCS) and lower aspartate aminotransferase, alanine aminotransferase and alkaline phosphatase activities than those of primiparous sows. The pluriparous sows showed mild hypoglycaemia and hypocholesterolaemia. When insulin was injected i.m. in the dose of 1 I.U. per kg body mass, a decrease in blood serum glucose, total protein and potassium levels was recorded 60 min later in both pluriparous and primiparous sows; a significant rise in 11-OHCS concentration occurred only in the primiparous sows. It was concluded that the response of the hypothalamo-pituitary-adrenal axis in pluriparous sows to an insulin load is reduced. Studies of pluriparous sows contribute to a better understanding of the ontogenetic development of pigs throughout their postnatal development.

Adrenal Cortex Hormones↗
PubMed1983

[Changes in selected biochemical indicators in blood serum in anestrous gilts].

The biochemical profile [levels of calcium, phosphorus, magnesium, chlorides and iron, the activities of alkaline phosphatase (ALP), aspartate aminotransferase (AST) and alanine aminotransferase (ALT), and the concentrations of total protein, albumin, cholesterol, urea, glucose, and vitamins A and E] was studied in the blood serum of 40 anoestrous and 40 control inseminated animals in a production herd with an increased occurrence of anoestrus in gilts. The anoestrous gilts showed significantly lower levels of albumin (P less than 0.01) and glucose (P less than 0.01) and ALP activity (P less than 0.05), and significantly higher concentrations of urea (P less than 0.01), vitamin A (P less than 0.01) and vitamin E (P less than 0.05) and ALT activity (P less than 0.05), as compared with the inseminated controls. An extended enzymatological examination consisting of the evaluation of the activities of ALP, AST, ALT and gamma-glutamyl transferase (GMT) was performed in another set of 22 anoestrous and 20 mated gilts. The anoestrous gilts showed a statistically significant increase in the activities of AST (P less than 0.01), GMT (P less than 0.01) and ALT (P less than 0.05) and an insignificant increase in the activity of ALP in comparison with the control animals. The comparison of the obtained values of the studied biochemical criteria with literary data indicated a lower concentration of magnesium and a higher ALP and ALT activities in the anoestrous and inseminated gilts in both groups under study. A high acidity of fat and a medium to high fungus infestation (Mucor sp., Aspergillus sp.) were found by chemical and mycological examination of the administered feed mixtures. The histological examination of the ovaries of anoestrous animals showed cystically degenerative changes, proliferations of fibrous elements, and partial atrophy of ovarial cortex. It has been inferred from the observations that mycotoxins may be involved in the increase in the occurrence of anoestrus, either by a direct effect on sexual organs or by impairing the function of liver which, secondarily contributes to the rise of ovarial dysfunctions.

Anestrus↗
PubMed1979

[Biochemical and hematological changes in sows during estrus].

After the second and fourth parturition of piglets, ten clinically healthy sows, weaned on the second day of life, were subjected to examination two to three days before heat, during heat (defined by typical changes on the outer genitals and immobilization in the presence of a boar) and two days after insemination. During the morning hours, four hours after the last feeding, blood was taken from V. cava cranialis. Seventeen parameters were determined in whole blood and in the blood serum. In the period of oestrus, compared with the period two to three days before this period and two days after insemination, a marked increase was observed in the concentration of the serum levels of 11-hydroxycorticosteroids, iron, and in the activity of alanine aminotransferase. The activity of aspartate aminotransferase was increased in heat only in comparison with the period of the two days after insemination. The concentration of haemaglobin in whole blood was significantly increased in comparison with the period before oestrus and after insemination. Further, in the heat period -- as compared with the time before it -- a considerable drop was observed in the content of inorganic phosphorus and vitamin A. The possible mechanism of the occurrence of other changes is discussed.

Animals↗
PubMed1979

[Changes in biochemical indicators in the blood and organs of pigs fed dried grape press cake].

In two experiments the effect of feeding dried crushed white and red grape press cake replacing 10--20% of the complex feed mixture A1 and SOL, was studied on the 21 biochemical indicators of blood serum, plasma, suprarenal glands, liver and tissue of fattened pigs. Changes indicating unsuitability of this non-traditional feed were not observed. During feeding red grape press cake, the young pigs of 35kg body weight had a lower concentration of glucose in blood serum, in comparison with the control. The temporary increase of calcium level and decrease of inorganic phosphorus in these animals was accompanied by a lower activity of alkaline phosphatase. White and red grape press cake affected positively the vitamin E level in blood serum. In the muscles of the experimental slaughter pigs protein proportion was increased and fat proportion was decreased.

Adrenal Glands↗
PubMed1976

Effect of glucose on adrenocortical activity of newborn piglets.

Adrenocortical function was followed in newborn piglets fasted for 36 hours and in piglets given only glucose or glucoplastic amino acids during this period. The greatest increase in relative adrenal weight, blood plasma 17-hydroxycorticosteroid (17-OHCS) levels and in the production of 17-OHCS by pig adrenals in vitro as compared with suckled controls was found in fasted piglets followed by amino acid-treated animals. The latter showed no significant differences in blood glucose between the initial and final values. The administration of glucose produced a transient hyperglycemia, failed to prevent the inhibition of body growth, resulted in a reduction in liver weight similar to that seen in fasted animals, and had a marked suppressive effect on the elevation of adrenocortical function. There were no significant differences in the production of 17-OHCS by adrenals in vitro per kg body weight between the glucose-treated and suckled piglets.

17-Hydroxycorticosteroids↗
PubMed1976

Effect of insulin hypoglycaemia on adrenocortical function and blood plasma vitamin E and cholesterol levels in suckling and weaned piglets.

Suckling piglets aged 28-31 days and weaned piglets aged 46-60 days were injected with insulin at a rate of 1 U/kg body weight to induce hypoglycaemia. All the animals were blood-sampled before and 45 and 90 minutes after the insulin injection. Both groups developed a marked adrenocortical response without any significant differences, though the rise in plasma 17-hydroxycorticosteroid (17-OHCS) levels of weaned piglets was slightly delayed. Plasma vitamin E concentration showed a significant rise in suckling piglets and a slight rise in weaned animals; it was not accompanied by an increase in total cholesterol levels. The results suggest that glucocorticoids affect vitamin E metabolism. The values of plasma 17-OHCS, cholesterol and vitamin E levels as well as of blood glucose levels and circulating eosinophil leucocyte counts confirmed differences in these parameters between suckling and weaned piglets.

17-Hydroxycorticosteroids↗
PubMed1976

[Effect of glucose and phenobarbital on the concentration of 11-hydroxycorticosteroids in the blood plasma of piglets].

In piglets 18 to 30 days old, the authors studied the changes of the levels of plasmatic 11-hydroxycorticosteroids (11-OHCS), the overall content of cholesterol and blood glucose two hours after oral application of glucose or phenobarbital. A dose of 0.5 g of glucose per kg of weight did not affect the parameters in question as compared with the control group which received a physiological solution. A dose of 2 g of glucose per kg of bodyweight caused glycaemia two hours after administration and an non-significant increase of the 11-OHCS level. Phenobarbital, administered at the rate of 10 mg per kg of bodyweight, markedly reduced the 11-OHCS level in the blood plasma. The level of total cholesterol showed a slight decrease in all instances. The authors discuss the mechanisms causing the changes observed. It is suggested that the effect of phenobarbital could be used for moderating the negative effects of the raised glucocorticoid level in stress situations.

11-Hydroxycorticosteroids↗