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Biomedical subjects

J Rak

Publications and source records attributed to J Rak.

At least 91 records · Page 5Linked to original sources
PubMed1988

Transplantable mouse 16/C mammary adenocarcinoma as a model in experimental cancer therapy. I. Kinetics of growth and spread.

Introduction of transplantable animal tumours has enabled the development of different model systems in which the site and type of tumour growth depends on the selection of suitable route for cells inoculation. Manipulation of the inoculation route of 16/C mammary adenocarcinoma results in local tumour growth either subcutaneous (s.c.), intramuscular (i.m.), intraperitoneal (i.p.) or intrasplenical (i.spl.) and development of metastases in lungs, lymph nodes, and liver. Exponential model of s.c. growth of the tumour was estimated and doubling time value calculated (3, 64 days). Correlation between the presence of tumour cells in lungs and advancement of s.c. growing tumours was found. Usefulness of 16/C adenocarcinoma as a model for experimental therapy is discussed.

Adenocarcinoma↗
PubMed1986

Antitumor activity of optical isomers of cyclophosphamide, ifosfamide and trofosfamide as compared to clinically used racemates.

The relationship between enantiomeric homogeneity of three oxazaphosphorine drugs: cyclophosphamide, ifosfamide and trofosfamide and their antitumor activity was evaluated by standard screening tests against four in vivo transplantable tumor models: L 1210 and P 388 lymphoid leukemias, Lewis lung carcinoma and 16/C line of mouse mammary adenocarcinoma. It was shown that the stereodifferentiation of anti-tumor effect of enantiomers was not outstanding although quite consistently in favour of levorotatory forms. The only exception was seen for cyclophosphamide enantiomers tested against leukemias where R/+/form was more effective than S/-/or racemate.

Animals↗
PubMed1986

Antitumor effect of selected daunorubicin derivatives in mice with P 388 leukemia.

Antitumor activity of four modified on amino sugar moiety derivatives of daunorubicin was compared with activity of two commercial preparations of the parental drug in tests against P 388 leukemia in mice. Tested analogs appeared to be similarly effective as the reference drugs, but two of them, DR-2 and DR-19, were found to be significantly less potent.

Animals↗
PubMed1986

Comparative studies on biological activity of /+/R and /-/S enantiomers of cyclophosphamide and ifosfamide. I. Antitumour effect of cyclophosphamide and ifosfamide enantiomers.

The differences in antitumour effect of /+/R and /-/S enantiomers of both cyclophosphamide and ifosfamide were detected. In the case of ifosfamide in all five tested tumour models (Leukemia L1210, P388, Lewis lung carcinoma, 16/C mammary adenocarcinoma and B16 melanoma) the /-/S form exerted not only higher antitumour effects than /+/R form, but revealed higher therapeutic indices as well. The same appeared to be true for /-/S enantiomer of cyclophosphamide in three models of solid tumours. In L1210 and P-388 ascitic leukemia models /+/R and /-/S cyclophosphamide exerted the same antitumour effect.

Animals↗
PubMed1985

Enhancement by cyclophosphamide of experimental pulmonary metastases formation of Lewis lung carcinoma. I. Dose-dependence and kinetics of cyclophosphamide effect.

The results presented in this study indicate that treatment of mice with Cyclophosphamide prior to Lewis lung carcinoma (LL2) cells inoculation enhanced the formation of artificial lung metastases in a dose-dependent manner. At the dose of Cyclophosphamide equal to 200 mg/kg the number of lung colonies was increased by a factor varying between 8 and 32. The effect was also time-dependent and it persists up to seventh day after Cyclophosphamide administration but was not visible when LL2 cells were injected 2 or 3 weeks later. Treatment of mice with Cyclophosphamide after LL2 cells inoculation did not enhance formation of tumor metastases.

Animals↗
PubMed1985

Enhancement by cyclophosphamide of experimental pulmonary metastases formation of Lewis lung carcinoma. II. Reduction of the cyclophosphamide effect by repopulation with cells from lymphoid organs.

Cyclophosphamide-induced enhancement of artificial lung metastasis of LL2 could be partly abolished by reconstituting of mice with spleen, lymph nodes or bone marrow cells but not with thymus cells. At cell dose of 10 x 10(6) per mouse the highest reconstituting capacity was found for spleen cells (73%) followed by bone marrow (60%) and lymph nodes cells (22%). Cell dose of 10(6) lymphoid cells per mouse did not reduce the CY-effect. The higher cell number (30 x 10(6) and 50 x 10(6) per mouse) did not abolish completely the CY-effect. The results indicate that in addition to sensitive lymphoid cells there exists another target for CY effect.

Animals↗
PubMed1984

Differentiation of pathogenic and saprophytic leptospira strains.

Comparative studies of 249 pathogenic and 80 saprophytic leptospira strains, including 2 strains of the illini type, using the 8-azaguanine test, growth at 13 degrees C and growth on trypticase soy broth revealed their good differentiating potency if the recommended conditions were carefully observed. The same results were obtained by a simple hemolytic test using sheep and rat blood cells, having the advantage of providing results within 24 h. This test is suggested to replace the 8-azaguanine and the growth test at 13 degrees C. In these investigations, the first European strain of the illini type was recognized.

Animals↗