Polypeptides isolated from ribosome-like structures occluded in avian myeloblastosis virus (AMV).
The protein composititon of ribosome-like particles isolated from AMV was determined by acrylamide gel electrophoresis and by immunological methods. It was established that the protein spectrum of ribosome-like particles differed significantly form the total protein spectrum of AMV. The most characteristic protein components of ribosome-like particles had a molecular weight in the range of 70 000--110 000. Apart from these proteins, the viral ribosomal particles contained a small amount of proteins with a molecular weight of 14 000--35 000 that could not be removed even by extensive purification. Immunological studies of the proteins of ribosome-like particles revealed the presence of antigenic determinants of ribosomal proteins. Furthermore, throughout the purification procedure the material contained components that reacted with antibodies against gs antigens.