Symposium on bacterial spores: XVI. Sporeformers as insecticides.
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Biomedical subjects
Publications and source records attributed to J R Norris.
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Crystalline inclusion bodies resembling those seen in Clostridium cochlearium were detected in cultures of Bacillus thuringiensis infected with bacteriophage.
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Baillie, Ann (University of Glasgow, Glasgow, Scotland), and John R. Norris. Antigen changes during spore formation in Bacillus cereus. J. Bacteriol. 87:1221-1226. 1964.-Ultrasonic extracts of Bacillus cereus cells harvested at different stages in spore formation were analyzed by immunoelectrophoresis with antisera prepared by injecting similar extracts into rabbits. Seven antigens resistant to heating at 80 C for 10 min were detected in extracts of young vegetative cells, and there were at least eight additional thermolabile antigens present at this stage. During spore formation, many of the thermolabile antigens disappeared from the cells and new antigens could be detected. Mature spores contained four of the heat-resistant antigens present in young vegetative cells and an additional five heat-resistant antigens. Only three thermolabile antigens were detected in spore extracts.
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Evidence from electron paramagnetic resonance (e.p.r.) studies suggests that the unpaired spin in oxidized P700+. or P865+. is shared by two special chlorophyll a (Chl a) or bacteriochlorophyll a (Bchl a) molecules respectively. Three classes of models have been proposed for special pair reaction centre chlorophyll: asymmetric, in which one Chl a (or Bchl a) acts as electron donor to a second acting as acceptor; models with translational symmetry only; and models with C2 symmetry. Models with C2 symmetry have been synthesized in vitro with two chlorophyll macrocycles tied together by a covalent link. The singlet and triplet states of the in vitro models have been characterized by e.p.r., nuclear magnetic resonance, and optical studies involving absorption, emission, and lasting behaviour. The fact that lasting occurs only from the folded configuration of the linked dimers suggests the availability of a highly effective nonradiative decay path from the S1 state of the excited open dimer. A radical-pair mechanism that accounts for the unusual spin polarization of the special pair triplet is proposed for the primary photochemistry in the reaction centre.
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This article documents the successful development of a computer-assisted process for systematic review of nursing policies and procedures at The Queen Elizabeth Hospital, Toronto, Ontario. Through broad staff input, policies and procedures are research-based yet grounded in the reality of the facility's nursing practice. Client-related standards are written as measurable, realistic statements of desired client outcomes, and as such are standards for client care. Policies and procedures have been rated according to the Canadian Council on Health Facilities Accreditation criteria (client centered, high risk, high volume, problem prone, interservice, outcome-related), so that important policies are easily identified to provide focus for quality assurance activities. The review process is structured so that over the course of a year, all policies are circulated for review by all levels of nursing staff.