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J R Jessen

Publications and source records attributed to J R Jessen.

6 recordsLinked to original sources
PubMed2001

[Relatives' attitude towards death in hospital].

INTRODUCTION: A survey was conducted over 13 months to measure the satisfaction level of relatives to patients, who had died in hospital. METHODS: The survey contained a questionnaire of 75 questions measuring satisfaction in different areas: the amount and quality of information given to the relatives by doctors, the support and care provided by the staff, and the quality of the physical environment. RESULTS: Out of 619 questionnaires posted, 462 were returned (74.6% response rate). Only half the relatives were satisfied with the level of information given by doctors to themselves and the one dying. Twenty-four per cent of the respondents were dissatisfied by the length of time taken by doctors to inform them about the situation. A broad majority of respondents (85%) were very satisfied with the care and support provided by the nursing staff. Sixty-six per cent of the patients died in private wards. Fifty-five per cent of the relatives were satisfied with the quality of the patient's room, but only 37% were satisfied with the environment offered to them. One in five was especially dissatisfied by the lack of a special room, which would permit enabling private conversations in an undisturbed environment. DISCUSSION: Our survey shows that doctors' communication with the relatives of dying patients needs to be improved. There also seems to be a need for providing a better environment for the relatives of dying patients by reserving separate rooms for rest and conversation.

Attitude to Death↗
PubMed2001

Concurrent expression of recombination activating genes 1 and 2 in zebrafish olfactory sensory neurons.

Each olfactory sensory neuron (OSN) expresses a single odorant receptor (OR) from a large repertoire of clustered OR genes. It has been hypothesized that OR gene regulation may involve stochastic DNA rearrangement, which in lymphocytes requires the recombination activating genes, rag1 and rag2. We have recently demonstrated that rag1 is expressed in zebrafish OSNs. Here we report that rag2, the obligate partner for rag1 function, is also expressed in OSNs and that its expression pattern mimics that of rag1. The onset of rag1 and rag2 expression preceded that of known zebrafish ORs and the number of rag1-positive OSNs corresponded with the number expressing the olfactory cyclic nucleotide-gated cation channel, an OSN marker. Zebrafish OSNs are the first example of concurrent rag expression in a nonlymphoid tissue. The expression of rag1 and rag2 in OSNs adds to the list of similarities between the olfactory and immune systems that includes monoallelic and mutually exclusive gene expression.

Animals↗
PubMed1999

Transgenesis.

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Animals↗
PubMed1998

Modification of bacterial artificial chromosomes through chi-stimulated homologous recombination and its application in zebrafish transgenesis.

The modification of yeast artificial chromosomes through homologous recombination has become a useful genetic tool for studying gene function and enhancer/promoter activity. However, it is difficult to purify intact yeast artificial chromosome DNA at a concentration sufficient for many applications. Bacterial artificial chromosomes (BACs) are vectors that can accommodate large DNA fragments and can easily be purified as plasmid DNA. We report herein a simple procedure for modifying BACs through homologous recombination using a targeting construct containing properly situated Chi sites. To demonstrate a usage for this technique, we modified BAC clones containing the zebrafish GATA-2 genomic locus by replacing the first coding exon with the green fluorescent protein (GFP) reporter gene. Molecular analyses confirmed that the modification occurred without additional deletions or rearrangements of the BACs. Microinjection demonstrated that GATA-2 expression patterns can be recapitulated in living zebrafish embryos by using these GFP-modified GATA-2 BACs. Embryos microinjected with the modified BAC clones were less mosaic and had improved GFP expression in hematopoietic progenitor cells compared with smaller plasmid constructs. The precise modification of BACs through Chi-stimulated homologous recombination should be useful for studying gene function and regulation in cultured cells or organisms where gene transfer is applicable.

Animals↗
PubMed1997

GATA-1 expression pattern can be recapitulated in living transgenic zebrafish using GFP reporter gene.

In this study, DNA constructs containing the putative zebrafish promoter sequences of GATA-1, an erythroid-specific transcription factor, and the green fluorescent protein reporter gene, were microinjected into single-cell zebrafish embryos. Erythroid-specific activity of the GATA-1 promoter was observed in living embryos during early development. Fluorescent circulating blood cells were detected in microinjected embryos 24 hours after fertilization and were still present in 2-month-old fish. Germline transgenic fish obtained from the injected founders continued to express green fluorescent protein in erythroid cells in the F1 and F2 generations. The green fluorescent protein expression patterns in transgenic fish were consistent with the pattern of GATA-1 mRNA expression detected by RNA in situ hybridization. These transgenic fish have allowed us to isolate, by fluorescence-activated cell sorting, the earliest erythroid progenitor cells from developing embryos for in vitro studies. By generating transgenic fish using constructs containing other zebrafish promoters and green fluorescent protein reporter gene, it should be possible to visualize the origin and migration of any lineage-specific progenitor cells in a living embryo.

Animals↗