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Biomedical subjects

J R Archer

Publications and source records attributed to J R Archer.

62 records · Page 4Linked to original sources
PubMed1978

Immune response inversion after hyperimmunisation. Possible mechanism in the pathogenesis of HLA-linked diseases.

The crosstolerance hypothesis suggests that animals sharing antigens with some microorganisms will produce low antibody levels in the early part and high levels in the latter part of an infection. Antibody responses have been measured in high responder B10.M and B10.D2 mice and low responder C3H and A.Thy-1.1, as well as F1 hybrids (B10.M X A.Thy-1.1) and (B10.M X C3H/He), after repeated immunisation with the antigen ferritin, involving altogether 483 mice. An inversion in the immune response was found to occur and similar delayed high antibody responses have been described in rheumatic fever. It is suggested a mechanism of immune inversion may operate in the pathogenesis of HLA and blood group-linked diseases.

Animals↗
PubMed1978

Antigen dose and strain variation as factors in the genetic control of the immune response to sperm whale myoglobin.

The primary and secondary immune response to the antigen sperm whale myoglobin was investigated in DBA/2, 129 and B10.BR mice over a dose range of immunization from 10 to 2000 microgram. Using an antigen excess technique, the quantity of antibody produced after secondary immunization followed a sigmoidal dose-response curve and the maximal plateau level was found to be different for each strain of mice. Furthermore, the genetic control of the immune response was investigated in twelve different inbred strains of mice following secondary immunization with 500 microgram of myoglobin. A continuous distribution for the mean antibody responses was obtained for the twelve different strains of mice. High responsiveness was associated with H-2 haplotypes d, f and k located on chromosome 17, the non-agouti gene 'a' located on chromosome 2 and the chinchilla gene 'c(ch)' located on chromosome 7. It is concluded that either a large number of IR-genes to myoglobin are present in many loci located on different chromosomes or the antibody differences could be explained by a cross-tolerance mechanism requiring no IR-genes at all.

Animals↗
PubMedDate not supplied

Factors influencing the cytotoxicity of anti-bacterial sera for lymphocytes from ankylosing spondylitis patients.

Rabbit antisera to certain strains of Gram-negative bacteria are reported to be cytotoxic for the lymphocytes of about 80% of HLA-B27 positive patients with ankylosing spondylitis (AS) but not for the lymphocytes of healthy HLA-B27 positive individuals. The lymphocytes of normal individuals can, however, be made susceptible to lysis by antibacterial sera by incubation in spent supernatant from appropriate bacterial cultures. In an attempt to explain the failure of certain laboratories to reproduce these results we have tested a number of variables in the 51Cr-release complement-dependent cytotoxicity assay. We conclude that AS patients in London and Sydney carry the same antigen, that several different incubation media can be used for both the cytotoxicity assay of HLA-B27 positive AS cells and modification of normal B27 positive cells and that the cells used may be collected either in heparin containing media or after defibrination. Major requirements for success include a healthy sample of lymphocytes (preferably at a fairly high concentration), suitable antiserum from a rabbit repeatedly immunised with large numbers of bacteria, appropriate complement and efficient technique. However, as we have failed to repeat this test consistently in London, even using materials tested in this study, it seems that another, probably non-biological, factor is also important.

Bacteria↗