Biomedical subjects

J Qu

Publications and source records attributed to J Qu.

At least 19 recordsLinked to original sources

PubMed2002

Ischemia alters the electrical activity of pacemaker cells isolated from the rabbit sinoatrial node.

The purpose of this study was to investigate the mechanisms responsible for ischemia-induced changes in spontaneous electrical activity. An ischemic-like Tyrode solution (pH 6.6) reversibly depolarized the maximum diastolic potential (MDP) and reduced the action potential (AP) overshoot (OS). We used SNARF-1, which is an indicator of intracellular pH (pH(i)), and perforated-patch techniques to test the hypothesis that acidosis caused these effects. Acidic but otherwise normal Tyrode solution (pH 6.8) produced similar effects. Basic Tyrode solution (pH 8.5) hyperpolarized the MDP, shortened the AP, and slowed the firing rate. In the presence of "ischemic" Tyrode solution, hyperpolarizing current restored the MDP and OS to control values. HOE-642, an inhibitor of Na/H exchange, did not alter pH(i) or electrical activity and did not prevent the effects of ischemic Tyrode solution or recovery after washout. Time-independent net inward current but not hyperpolarization-activated inward current was enhanced by ischemic Tyrode solution or by 30 microM BaCl(2), a selective blocker of inward-rectifying K currents at this concentration. The results suggest that 1) acidosis was responsible for the ischemia-induced effects but Na/H exchange was not involved, 2) the OS was reduced because of depolarization-induced inactivation of inward currents that generate the AP upstroke, and 3) reduction of an inward-rectifying outward K current contributed to the depolarization.

Action Potentials↗

PubMed2001

Insight into hepatocellular carcinogenesis at transcriptome level by comparing gene expression profiles of hepatocellular carcinoma with those of corresponding noncancerous liver.

Human hepatocellular carcinoma (HCC) is one of the most common cancers worldwide. In this work, we report on a comprehensive characterization of gene expression profiles of hepatitis B virus-positive HCC through the generation of a large set of 5'-read expressed sequence tag (EST) clusters (11,065 in total) from HCC and noncancerous liver samples, which then were applied to a cDNA microarray system containing 12,393 genes/ESTs and to comparison with a public database. The commercial cDNA microarray, which contains 1,176 known genes related to oncogenesis, was used also for profiling gene expression. Integrated data from the above approaches identified 2,253 genes/ESTs as candidates with differential expression. A number of genes related to oncogenesis and hepatic function/differentiation were selected for further semiquantitative reverse transcriptase-PCR analysis in 29 paired HCC/noncancerous liver samples. Many genes involved in cell cycle regulation such as cyclins, cyclin-dependent kinases, and cell cycle negative regulators were deregulated in most patients with HCC. Aberrant expression of the Wnt-beta-catenin pathway and enzymes for DNA replication also could contribute to the pathogenesis of HCC. The alteration of transcription levels was noted in a large number of genes implicated in metabolism, whereas a profile change of others might represent a status of dedifferentiation of the malignant hepatocytes, both considered as potential markers of diagnostic value. Notably, the altered transcriptome profiles in HCC could be correlated to a number of chromosome regions with amplification or loss of heterozygosity, providing one of the underlying causes of the transcription anomaly of HCC.

Carcinoma, Hepatocellular↗

PubMed2001

Synthesis and characterization of chiral N-O turns induced by alpha-aminoxy acids.

Chiral alpha-aminoxy acids of various side chains were synthesized with high optical purity starting from chiral alpha-amino acids. The conformations of diamides 13a-e, 15, and 16 were probed by using NMR, FT-IR, and CD spectroscopic methods as well as X-ray crystallography. The right-handed turns with eight-membered-ring intramolecular hydrogen bonds between adjacent residues (called the N-O turns) were found to be preferred for D-aminoxy acid residues, and they were independent of the side chains. The rigid chiral N-O turns should have great potential in molecular design.

Amino Acids↗

PubMed2001

Identification and determination of glucuronides and their aglycones in Erigeron breviscapus by liquid chromatography-tandem mass spectrometry.

A novel, speedy and reliable LC-MS-MS method for the search, identification and quantitation of O-glycosides and their aglycones from plant resources was established by analyzing the extract of Erigeron breviscapus. The extract was directly infused to a triple-quadrupole MS-MS and major glucuronides in the extract were screened out with high confidence by a neutral loss scan for the loss of a gluconic acid. The identity of these glucuronides and their aglycones was further confirmed with LC-MS-MS. In addition to scutellarin, apigenin 7-O-glucuronide, quercetin-3-O-glucuronide and their aglycones that were previously reported by others, we also confirmed by LC-MS-MS that remarkable amount of baicalin, an isomer of apigenin 7-O-glucuronide, presented in the extract of this plant, which had not been reported before. A satisfying quantitation of three glucuronides was also made by LC-MS-MS.

Asteraceae↗

PubMed2001

HCN2 overexpression in newborn and adult ventricular myocytes: distinct effects on gating and excitability.

Ventricular pacemaker current (I(f)) shows distinct voltage dependence as a function of age, activating outside the physiological range in normal adult ventricle, but less negatively in neonatal ventricle. However, heterologously expressed HCN2 and HCN4, the putative molecular correlates of ventricular I(f), exhibit only a modest difference in activation voltage. We therefore prepared an adenoviral construct (AdHCN2) of HCN2, the dominant ventricular isoform at either age, and used it to infect neonatal and adult rat ventricular myocytes to investigate the role of maturation on current gating. The expressed current exhibited an 18-mV difference in activation (V(1/2) -95.9+/-1.9 in adult; -77.6+/-1.6 mV in neonate), comparable to the 22-mV difference between native I(f) in adult and neonatal cultures (V(1/2) -98.7 versus -77.0 mV). This did not result from developmental differences in basal cAMP, because saturating cAMP in the pipette caused an equivalent positive shift in both preparations. In the neonate, AdHCN2 caused a significant increase in spontaneous rate compared with control (88+/-5 versus 48+/-4 bpm). In adult, where HCN2 activates more negatively, the effect was evident only during anodal excitation, requiring significantly less stimulus energy than control (2149+/-266 versus 3140+/-279 mV. ms). Thus, ventricular maturational state influences the voltage dependence of expressed HCN2, resulting in distinct physiological impact of expressed channels in neonate and adult myocytes. The full text of this article is available at http://www.circresaha.org.

Animals↗

PubMed2001

Determination of scutellarin in Erigeron breviscapus extract by liquid chromatography-tandem mass spectrometry.

A quantitative assay for scutellarin by LC-MS-MS (negative ion mode) was developed. The scutellarin was extracted from dry Erigeron breviscapus. Significant ion suppression was observed, which could be eliminated by increasing the turboionspray interface temperature to 350 degrees C and by 1000-fold dilution of the extract with solvent. The calibration curve of scutellarin showed excellent linearity over a wide concentration range (0.01-100 microg/ml) (r=0.998), and the limit of detection was 15 pg/ml using a 10-microl injection volume. The analysis time was 4 min/sample.

Asteraceae↗

PubMed2001

The serine/threonine kinase PAK4 prevents caspase activation and protects cells from apoptosis.

The serine/threonine kinase PAK4 was identified first as an effector molecule for the Rho GTPase Cdc42. PAK4 differs from other members of the PAK family both in sequence and function. Previously we have shown that an important function of this kinase is to mediate the induction of filopodia in response to activated Cdc42. Studies with a constitutively active PAK4 mutant have shown that it also has a role in promoting anchorage-independent growth, an important hallmark of oncogenic transformation. Here we show that another function of PAK4 is to protect cells against apoptotic cell death. Expression of wild-type or constitutively active PAK4 delays the onset of apoptosis in response to tumor necrosis factor alpha stimulation, UV irradiation, and serum starvation. Consistent with an antiapoptotic function, expression of PAK4 leads to an increase in phosphorylation of the proapoptotic protein Bad and an inhibition of caspase activation.

3T3 Cells↗

PubMed2001

Comparison of blood counts in venous, fingertip and arterial blood and their measurement variation.

We compared routine blood pictures in venous, fingertip and arterial blood and their measurement variation. A pair of venous and fingertip blood samples were simultaneously collected from 24 volunteers, and another pair of venous and arterial samples from another 12 volunteers. The volunteers were healthy adults, aged 20-22 years, with males and females in equal numbers. Three aliquots of blood were taken from each person for three measurements of blood counts, including haemoglobin concentration and haematocrit, using an automatic haematology analyser. Compared to arterial blood, there was a significant increase of erythrocyte count (2.7%) and haematocrit (3.1%) in the venous blood. The total and large leucocyte counts were significantly higher (9.2% and 12.6%, respectively) in the fingertip blood than in the venous blood. There were a decreasing number of leucocytes in the fingertip blood with repeated sampling. The intermeasurement variation for total leucocyte or haemoglobin measurement was significantly larger as measured with the fingertip blood or arterial blood than with the venous blood. The intermeasurement coefficients of variation of haemoglobin (1.3%) and small leucocyte ratio or platelet (approximately 4.0%) measurements with venous and arterial blood were the smallest and largest, respectively. Volume of arterial plasma was diminished by 2-3% after microcirculation. Elevation of total leucocyte count in the fingertip blood was mainly due to the increase of granulocytes. Measurement with venous blood was of better precision than with fingertip or arterial blood.

Adult↗

PubMed2001

Time-frequency representations of Lamb waves.

The objective of this study is to establish the effectiveness of four different time-frequency representations (TFRs)--the reassigned spectrogram, the reassigned scalogram, the smoothed Wigner-Ville distribution, and the Hilbert spectrum--by comparing their ability to resolve the dispersion relationships for Lamb waves generated and detected with optical techniques. This paper illustrates the utility of using TFRs to quantitatively resolve changes in the frequency content of these nonstationary signals, as a function of time. While each technique has certain strengths and weaknesses, the reassigned spectrogram appears to be the best choice to characterize multimode Lamb waves.

Journal Article↗

PubMed2001

Crack characterization using guided circumferential waves.

This paper examines the propagation of guided circumferential waves in a hollow isotropic cylinder that contains a crack, with the goal of using these guided waves to both locate and size the crack. The crack is sized using a modified Auld's formula, which relates the crack's length to a reflected energy coefficient. The crack is then located by operating on the backscattered signal with a time-frequency digital signal processing (DSP) technique, and then comparing these results to those obtained if the cylinder is perfect. The guided circumferential waves are generated with a commercial finite element method (FEM) code. One objective of this work is to demonstrate the effectiveness of using sophisticated DSP techniques to describe the effect of scattering on dispersive waves, showing it is possible to characterize cracks systematically and accurately by quantifying this scattering effect. The results show that the need for high frequency signals to detect small cracks is significantly decreased by using these techniques.

Journal Article↗

PubMed2001

Activated PAK4 regulates cell adhesion and anchorage-independent growth.

The serine/threonine kinase PAK4 is an effector molecule for the Rho GTPase Cdc42. PAK4 differs from other members of the PAK family in both sequence and function. Previously we have shown that an important function of this kinase is to mediate the induction of filopodia in response to activated Cdc42. Since previous characterization of PAK4 was carried out only with the wild-type kinase, we have generated a constitutively active mutant of the kinase to determine whether it has other functions. Expression of activated PAK4 in fibroblasts led to a transient induction of filopodia, which is consistent with its role as an effector for Cdc42. In addition, use of the activated mutant revealed a number of other important functions of this kinase that were not revealed by studying the wild-type kinase. For example, activated PAK4 led to the dissolution of stress fibers and loss of focal adhesions. Consequently, cells expressing activated PAK4 had a defect in cell spreading onto fibronectin-coated surfaces. Most importantly, fibroblasts expressing activated PAK4 had a morphology that was characteristic of oncogenic transformation. These cells were anchorage independent and formed colonies in soft agar, similar to what has been observed previously in cells expressing activated Cdc42. Consistent with this, dominant-negative PAK4 mutants inhibited focus formation by oncogenic Dbl, an exchange factor for Rho family GTPases. These results provide the first demonstration that a PAK family member can transform cells and indicate that PAK4 may play an essential role in oncogenic transformation by the GTPases. We propose that the morphological changes and changes in cell adhesion induced by PAK4 may play a direct role in oncogenic transformation by Rho family GTPases and their exchange factors.

Animals↗

PubMed2001

[The efficiency of algae removal from drinking water by ferrate].

This paper studied the efficiency of algae removal by ferrate from two kinds of raw water which were collected from Donghu and Tiegang reservoir respectively. The experimental results showed that the removal efficiency of algae reached about 95% only through PAC coagulation when the raw water was extracted from Donghu reservoir in which most of algae was Chlorella. But dealing with Tiegang raw water in which the majority of algae were Oscillatoria, terrate was used as pre-oxidant coupling with PAC coagulation not only using PAC to flocculate and the efficiency of algae removal could be up to 97.85%. However this treatment method using ferrate as pre-oxidant overmatched the traditional method prechlorination for algae removal.

Eukaryota↗

PubMed2001

Alteration of surfactant proteins A and D in bronchoalveolar lavage fluid of Pneumocystis carinii pneumonia.

OBJECTIVE: To understand the interaction between surfactant proteins and pneumocystis carinii pneumonia (PCP), and the impact of corticosteriods on surfactant proteins. METHODS: We established rat models of PCP and bacterial pneumonia induced by subcutaneous injection of 25 mg cortisone acetate. At 8-12 wk, the bronchoalveolar lavage fluid (BALF) of rats was collected. Total nucleated cells of BALF were counted and differentiated, and the concentrations of surfactant protein A (SP-A) and surfactant protein D (SP-D) were measured by immunoblotting assay. The rats were divided into three immunosuppressive groups and a normal control group. Group I, normal control (n = 6), consisted of healthy SD rats; group II, negative control (n = 6), consisted of rats with cortisone acetate injection for over 8 wk without lung infection; group III, bacterial pneumonia (n = 11), rats were injected with cortisone acetate over 8 wk that resulted in bacterial pneumonia without other pathogens isolated; and group IV, PCP (n = 14), rats with injected cortisone acetate for 8-12 wk and developed PCP without other pathogens isolated. RESULTS: Our results indicated that the total cell count in BALF in the negative control group was lower than that in the normal control group (P < 0.001). During PCP infection, the total cell count and the percentage of polymorphonuclearcytes (PMNs) in BALF were significantly increased (P < 0.01), but were lower than those in the bacterial pneumonia group. The concentration of SP-A of BALF in PCP (45.1 +/- 22.1 micrograms/ml) was significantly increased in comparison with that in the negative control (16.2 +/- 9.9 micrograms/ml, P < 0.05) and bacterial pneumonia groups (6.2 +/- 5.6 micrograms/ml, P < 0.001). We also found that the relative content of SP-D was significantly higher in PCP (24,249 +/- 4780 grey values) than that in the negative control (13,384 +/- 2887 grey values, P < 0.001) and that in bacterial pneumonia (11,989 +/- 2750 grey values, P < 0.001). SP-A and SP-D were also higher in the moderate to heavy group of PCP than those seen in the mild group (P < 0.01, P < 0.001). SP-A and SP-D were higher in the negative control group than those in the normal control group, but there was no significant difference between the 2 groups. CONCLUSION: These results suggest that the concentrations of SP-A and SP-D in BALF are increased by pneumocystis carinii specific stimulation, but the alteration is not related to the corticosteriod usage.

Animals↗

PubMed2001

[Telomerase activity in stool of patients with colorectal cancer].

OBJECTIVE: To probe into the method for screening colorectal cancer by the way of stool. METHODS: Telomerase activity in the stool of patients with colorectal cancer was determined by PCR-TRAP. RESULTS: The positive rate of telomerase activity in the stool of patients with colorectal cancer was 62.8%. Its sensitivity, specificity and positive predictive value were 62.8%, 95.7%, and 96.4%, respectively. Telomerase activity in stool was not obviously correlated with Duke's stage, lymphatic metastasis and tumor location. Telomerase activity was also found in the stool sample of a patient with colonic adenoma. CONCLUSION: The determination of telomerase activity in the stool of patients with colorectal cancer contributes to the improvement of screening and diagnosis of colorectal cancer.

Colorectal Neoplasms↗

PubMed2001

[The effectiveness of prolene patch in hernia prevention following harvesting rectus abdominis myocutaneous flap].

OBJECTIVE: To investigate the effectiveness of polypropylene path (Prolene) in hernia prevention following harvesting of rectus abdominis is myocutaneous flap. METHODS: From November 1999 to October 2000, Prolene patches were applied in 26 cases to repair the anterior rectus sheath following harvesting free rectus abdominis myocutaneous flap. Data concerning each case included size of rectus flap, defect of rectus sheath, size of patch used, wound healing and complications. RESULTS: Prolene patch showed good biocompatibility with abdominal tissue. No foreign-body rejection occurred after operation. Seroma developed in 1 case, and was drained bedside without complication. All prolene patches healed well in the body during follow-up. Hernia formation and abdominal bulge were not observed. CONCLUSION: Prolene patch is a satisfactory material for repair of the anterior rectus sheath after harvesting free rectus abdominis myocutaneous flap.

Adolescent↗

PubMed2001

[Alterations of pulmonary surfactant during Pseudomonas aeruginosa pneumonia of immunocompromised rats].

OBJECTIVE: To analyze the alterations of pulmonary surfactant in immunocompromised host with Pseudomonas aeruginosa (PA) pneumonia. METHODS: 50 rats were randomly divided into two groups, one immunosuppressed with cyclophosphamide and cortisone acetate as ICH group, another as control group (CON), their lung tissue and bronchoalveolar lavage fluid (BALF) were collected before PA challenging and 3 h, 6 h, 9 h, 24 h after PA challenging intratracheally, wet/dry ratio (W/D) of lung tissue were measured, concentrations of total protein (TP), total phospholipids (TPL), disaturated phosphatidylcholine (DSPC) and surfactant protein A (SP-A) in BALF were analyzed. RESULTS: The ratios of DSPC/TPL and DSPC/TP decreased significantly in both ICH and CON groups after PA infection, there were not significant differences between the two groups, no change in the concentrations of TPL and DSPC. Concentration of SP-A and SP-A/TP in ICH group decreased remarkably 6 h after PA pulmonary infection than before [(1.8 +/- 1.1) microgram/ml vs (4.2 +/- 1.5) microgram/ml, (1.4 +/- 0.7) microgram/mg vs (11.7 +/- 8.1) microgram/mg, P < 0.05], meanwhile there were no significant alterations in CON group. W/D and TP concentrations increased in both groups after PA challenging, however the alterations were much greater in ICH group (P < 0.05). Alterations of SP-A appeared a negative correlation with alterations of TP and W/D (r = -0.793, P < 0.01, r = -0.769, P < 0.01). The ratios of SP-A/TPL and SP-A/DSPC decreased significantly 6 h after PA challenging than before, the ratios were much lower in ICH group than in CON group during 6 h-9 h after PA inoculation. CONCLUSION: After PA pulmonary infection, alterations of phospholipids could initially appear a relative decrement of DSPC during acute phase of infection, much remarkable decrease of SP-A in ICH group could be associated with the more severe lung injury, alterations of SP-A were more obvious than surfactant lipids in ICH.

Animals↗

PubMed2001

[Autotrophic denitrification of groundwater by electrochemical process].

An autotrophic denitrification process and its reactor for removing nitrate from groundwater was investigated in this paper. Using activated carbon fiber(ACF) as electrodes, the electrochemical reactions could produce hydrogen as the donor for autotrophic denitrification. In the process, nitrate was removed effectively and no accumulation of nitrite in the effluent. The results in this study proved that some key factors, such as the applied current density, flow rate of water, oxidation-reduction potential and the nitrate concentration in raw water, influenced the electrochemically denitrifying effect. When the concentration of NO3(-)-N was 28.8 mg/L in inlet water, the optimum applied density was 9 mA, the highest hydraulic load of the reactor was 35 ml/h, and the reactor performed a practical buffering capacity to pH. After applying electricity to the reactor for 1 h, the oxidation-reduction potential decreased to below--200 mA, so an adaptable reductive environment could be provided in the reactor for denitrification.

Carbon↗

PubMed2000

Inactivation of monomeric sarcosine oxidase by reaction with N-(cyclopropyl)glycine.

Monomeric sarcosine oxidase (MSOX) catalyzes the oxidative demethylation of sarcosine (N-methylglycine) and contains covalently bound flavin adenine dinucleotide (FAD). The present study demonstrates that N-(cyclopropyl)glycine (CPG) is a mechanism-based inhibitor. CPG forms a charge transfer complex with MSOX that reacts under aerobic conditions to yield a covalently modified, reduced flavin (lambda(max) = 422 nm, epsilon(422) = 3.9 mM(-1) cm(-1)), accompanied by a loss of enzyme activity. The CPG-modified flavin is converted at an 8-fold slower rate to 1,5-dihydro-FAD (EFADH(2)), which reacts rapidly with oxygen to regenerate unmodified, oxidized enzyme. As a result, CPG-modified MSOX reaches a CPG-dependent steady-state concentration under aerobic conditions and reverts back to unmodified enzyme upon removal of excess reagent. No loss of activity is observed under anaerobic conditions where EFADH(2) is formed in a reaction that goes to completion at low CPG concentrations. Aerobic denaturation of CPG-modified enzyme yields unmodified, oxidized flavin at a rate similar to the anaerobic denaturation reaction, which yields 1,5-dihydro-FAD. The CPG-modified flavin can be reduced with borohydride, a reaction that blocks conversion to unmodified flavin upon removal of excess CPG or enzyme denaturation. The possible chemical mechanism of inactivation and structure of the CPG-modified flavin are discussed.

Aerobiosis↗