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Biomedical subjects

J Q Li

Publications and source records attributed to J Q Li.

At least 37 records · Page 2Linked to original sources
PubMed1997

[Effect of age and ginsenoside Rg1 on nitric oxide content and nitric oxide synthase activity of cerebral cortex in rats].

Nitric oxide(NO) content and nitric oxide synthase(NOS) activity were measured in cerebral cortex isolated from Wistar rats of three age groups(young: 3 months; adult: 9 months; and old: 27 months). No significant differences in NO content and NOS activity between young and adult rats were found(P > 0.05). The NO content and NOS activity in old rats were shown to be significantly higher than those of young and adult rats(P < 0.01). When treated with Rg1(10, 20, 40 mg.kg-1), the NO content and NOS activity in old rats decreased. The inhibitory effect of Rg1 on NOS was found to be dose-dependent in the range of 10-40 mg.kg-1. The optimal reduction in NO content and NOS activity induced by Rg1 occurred at 40 mg.kg-1 for old rats(P < 0.01). In view of the close relationship of NO content and NOS activity with aging, the inhibitory effect of Rg1 on NOS activity, as shown by our results, might provide an explanation for its antiaging function.

Aging↗
PubMed1997

[Study on the anti-apoptotic mechanism of ginsenoside Rg1 in cultured cortical neurons].

Ginsenoside Rg1 is an active principle isolated from Panax ginseng CA Meyer. Primary neuronal culture was performed with different concentrations of Rg1. On the 14th day, the culture was changed to serum-free medium. On the 16th day, neurons were harvested and assayed under microscope and fluorescence microscope. The result showed that serum withdrawal from the medium induced apoptosis in primary cultured cortical neurons. Rg1 (1, 10 mumol.L-1) was shown to inhibit apoptosis and protect neurons against injury. Membrane fluidity was measured using fluorescence spectrophotometer in five groups of cultured cortical neurons: control (complete medium), apoptosis (serum-free medium), Rg1 0.1 mumol.L-1, Rg1 1 mumol.L-1 and Rg1 10 mumol.L-1. Neurons were isolated enzymatically and loaded with the fluorescent dye, DPH (1, 6-diphenyl-1,3,5-hexatriene). Membrane fluidity in control neurons (eta 1.2928 +/- 0.1426) was shown to be significantly higher than that in apoptosis cells (eta 2.9277 +/- 0.2004). The membrane fluidity of neurons treated with Rg1 (0.1, 1, 10 mumol.L-1) increased significantly (eta 2.9172 +/- 0.1604, 2.4731 +/- 0.2187, 1.6436 +/- 0.1483). These findings indicate a substantial alteration of membrane fluidity with neuronal apoptosis. Increment of membrane fluidity provides an aspect of elucidating the mechanism of Rg1's anti-apoptosis function.

Animals↗
PubMed1995

Randomized study of chemoembolization as an adjuvant therapy for primary liver carcinoma after hepatectomy.

From April 1990 to December 1993, 140 patients were recruited to a randomized study to evaluate transcatheter hepatic arterial chemoembolization (TACE) as an adjuvant therapy for primary liver carcinoma after hepatectomy. This study investigated the principle, techniques and results of TACE. The results showed that the intrahepatic recurrence rate was 48.9% in the patients who underwent radical resection only, but only 21.3% in the patients who also underwent TACE 3-4 weeks after hepatectomy (P < 0.01). The 1-, 2-, 3-, and 4-year survival rates were 72.3%, 52.7%, 35.1%, and 35.1% respectively for the patients who underwent radical resection only, and were 97.9%, 85.5%, 69.5%, and 56.9% for the patients who also underwent TACE 3-4 weeks after radical resection (P < 0.001). The 1-, 2-, 3-, and 4-year survival rates were 38.9%, 0%, 0%, and 0% for the patients who underwent palliative resection only, and were 68.3%, 32.3%, 21.5%, and 21.5% respectively for the patients undergoing TACE 3-4 weeks after palliative hepatectomy (P < 0.001).

Adult↗
PubMed1994

Cation channel blocked by extracellular Ca2+ in the apical membrane of the chick embryonic ectoderm.

In the chick embryo (20 h incubation, gastrula stage), the apical membrane of the ectodermal cells shows a high density of a non-selective cation channel which is blocked by very low extracellular Ca2+ concentrations. Properties of this channel were studied at the single-channel level using the patch-clamp technique in the cell-attached mode. With 1 mmol/l Ca2+ in the pipette, only outward current was present and the channel conductance measured at +120 mV was 25.5 pS. In the absence of Ca2+, also inward current through the channel was observed. The conductances measured at -50 mV were 49.5 pS with Na+ as the charge carrier, 72.5 pS with K+, 49.1 pS with Cs+, and 18.5 pS with Li+. The conductance measured at +80 mV was around 23 pS in all four cases. The reversal potential was similar (around 25 mV) for all four ions, which indicates a poor selectivity of the channel. In the absence of Ca2+ and the presence of 1 mmol/l ethylenebis(oxonitrilo)tetraacetate (EGTA), the kinetics of the channel were characterized by bursts of the order of seconds. During a burst, the channel flickered between one open and one closed level. The open time was constant between -30 mV and -80 mV, while the closed time decreased with hyperpolarization. The open time varied according to the permeant ion (K+ < Na+ = Cs+ < Li+). Extracellular Ca2+ blocked the inward current in a voltage-dependent manner. The Kd values, 1 mumol/l at -30 mV and 3.2 mumol/l at -80 mV, indicate that Ca2+ ions exit the channel toward the intracellular side. A weak voltage dependency of the association rate constant suggests that the Ca(2+)-binding site is close to the outside mouth. Extracellular Ca2+ was much less efficient at blocking the outward current (Kd about 1 mmol/l at 80 mV). Tetracaine, but not uraniumdioxide, decreased the opening probability of the channel. The embryonic channel shows similarities with the Ca(2+)-blockable, poorly selective channel described in the epithelium of toad urinary bladder.

Animals↗
PubMed1994

[Evaluation of chemoembolization as an adjuvant therapy for primary liver carcinoma after surgical resection].

In order to decrease the intrahepatic recurrence rate after surgical resection of hepatoma, from October 1989 to December 1992, 219 patients were recruited to a prospective trial to evaluate transcatheter hepatic arterial chemo-embolization (TCE) as a postoperative adjuvant therapy for primary liver carcinoma. These patients who underwent radical or palliative resection were divided into 3 groups: (1) Resection of tumor; (2) TCE three to four weeks after resection; (3) Relapse after resection plus TCE. This study presented the principle, techniques and results of TCE. The intrahepatic recurrence rate was 58.6% in group I and 9.5% in group II (P < 0.001). The 1-, 2-, 3-year-survival rates were 67.2%, 52.0%, 42.8% of the patients in group I, while 95.2%, 84.7%, 67.7% in the patients in group II (P < 0.05). The 1-, 2-, 3-year-survival rates were 21.5%, 0%, 0% of the patients who underwent palliative. resection only, while 66.7%, 41.7% of the patients in group III (P < 0.001). Taken together, adjuvaut chemoembolization helps decrease the recurrence rate and increase the survival rate in patients with primary liver cancer treated with surgical resection.

Adult↗
PubMed1994

[Long-term results of irregular hepatectomy for primary liver cancer at the right lobe].

From 1964 to 1990, 228 cases with primary liver cancer at the right lobe underwent irregular hepatectomy in our hospital. Of them, 47 were asymptomatic. Alpha feto-protein (AFP) was positive in 162 (71%) and 195 patients were associated with liver cirrhosis (85.6%). In 60 cases, the tumor size was less than 5cm in diameter. During operation, the following methods were used for interruption of hepatic blood flow: temporary blockage of the hepatic hilum in 117 cases, microwave coagulation of hepatic parenchyma in 77 cases, continuous mattress sutures in 29 cases, hepatic clamp in 3 cases and other methods in 2 cases. One segment resection was done in 116 patients, two segments in 67, three segments in 28 and four segments in 17. One hundred and fifty-five patients underwent radical resection and 73 patients underwent palliative resection. The operative mortality was 2.6%. The 3- and 5- year survival rates of the patients so treated were 48.3% and 38.8%, respectively. The 3- and 5- year survival rates of the patients with small tumor ( < 5cm in diameter) after hepatectomy were 59.9% and 53.3%, respectively. The medvan survival time after palliative hepatectomy was 15.7 months.

Adult↗
PubMed1994

[Clinical factors affecting prognosis of small hepatocellular carcinoma after hepatectomy].

One hundred and two patients with histologically diagnosed small hepatocellular carcinoma (< or = 5cm HCC) underwent curative irregular hepatectomy in our hospital from Nov 1972 to Nov 1992. The postoperative survival rates at 1,3,5 and 10 years were 93.9%, 75.3%, 52.8% and 44.6%, respectively. We analysed the clinical factors using a Cox model and found that the clinical staging of HCC, gamma-GT, satellite tumor nodules, tumour capsule and postoperative recurrence have significant effect on the survival rate of patients. We conclude that early diagnosis of sub--clinical HCC, transarterial embolism after hepatectomy to reduce postoperative recurrence, and active treatment with operation and/or TAE after recurrence are effective methods to improve survival of HCC patients.

Adult↗
PubMed1994

[Clinical study on epidermal growth factor and its receptor in human gastric cancer].

We detect the expression of epidermal growth factor (EGF) and epidermal growth factor receptor (EGFR) in 104 specimens of gastric cancer by avidin biotin-peroxides complex technique (ABC). The positive rates of EGF, EGFR and synchronous EGF and EGFR were 35.6%, 42.3%, 30.8% respectively. The positive expression of EGF and EGFR to gastric cancer tissue was inclined to occur in patients who were in advanced stages, of poorly-differentiated types, Borrmann III, IV types, scirrhous type, seroinvasive type or lymph node metastasic types. The survival rates at 1, 3, 5 years after gastrectomy in the patients with expression of EGF were significantly lower than the survival rates of the patients with negative expression (P < 0.05). The patients with synchronous expression of EGF and EGFR had the worst prognosis. After gastrectomy, all of them died within 4 years. It is indicated that EGF and EGFR could serve as biological indicators of gastric cancer malignancy and an index for evaluating, the prognosis of the patients with gastric cancer.

Adenocarcinoma↗
PubMed1992

The formation and crystal structure of dihydronitidine and discussion of anticancer mechanism of nitidine cation.

The paper reports the formation and crystal structure of dihydronitidine, expounds the reasons and conditions of easily formed oxynitidine, and discusses anticancer mechanism of nitidine (cation). The crystallographic parameters of dihydronitidine are: space group P(2)1/n, a = 12.54(1), b = 9.148(5), c = 14.748(8) A, beta = 92.12(6)degrees, Z = 4. 4108 independent reflections were collected within the range of 3 degrees < or = 2 theta < or = 54 degrees, of which 2137 intensity data with I > or = 3 sigma (I) were used in the structural determination. The crystal structure has been refined by full matrix least-square method to a final R of 0.050.

Antineoplastic Agents, Phytogenic↗
PubMed1990

Localization of small liver tumors.

Thirty-six patients with small liver tumor were diagnosed by alpha-fetoprotein (AFP); sonography, and computed tomography (CT), and underwent hepatectomy. The pathological types included 23 hepatocelluler carcinoma (HCC), 11 hepatic cavernous hemangioma, and 2 secondary liver cancer. In 22 patients, the tumor nodules were located in the right lobe and 14 cases in the left lobe. The diagnostic accuracy rate of CT was 100% for HCC and secondary liver cancer, but for hepatic cavernous hemangioma it was only 72.2%. However, the accuracy rate of sonography was as high as 81.8% for hepatic cavernous hemangioma and only 60.4% for liver malignancies. The positive rate of AFP for the HCC patients of this series was only 66.6%. The method of intraoperative detection of small liver tumor is introduced, if the tumor was invisible grossly or nonpalpable during exploratory laparotomy. In the series, 7 cases in whom the right lobe lesion was too small to be located by routine manual examination during exploratory laparotomy were detected by this method, and all small liver tumors were resected successfully.

Adult↗
PubMed1989

Evaluation of partial hepatectomy for primary liver carcinoma.

From 1964 to 1985, 120 cases of primary liver carcinoma had undergone hepatectomy in our hospital. Seven of these cases underwent hepatic lobectomy, 28 cases had palliative hepatic resection, and 85 cases had partial hepatectomy. We introduced different surgical modes and compared the mortality, survival rates, and complications between the hepatic lobectomy and partial hepatectomy groups. The mortality rates of these two groups were 14.3 and 3.5%, respectively, and the 1, 3, and 5 year survival rates were 83.3, 33.3, and 16.7 and 68.8, 48.1, and 20%, respectively. In the palliative hepatectomy group (28 cases), ten cases received combined radiotherapy postoperation. Most of these cases died during the first year postoperation. Primary liver cancer in Asia is commonly associated with hepatic cirrhosis. We suggest that partial hepatectomy is suitable for such patients. The results of the present series showed that the outcomes of the hepatic lobectomy and partial hepatectomy groups did not differ, but in partial hepatectomy, the operative mortality and complications were reduced, bleeding minimized, and operation time also shortened.

Adenoma, Bile Duct↗
PubMed1989

Subclinical primary liver carcinoma.

From 1976 to 1984, 30 cases of asymptomatic primary liver cancer (subclinical primary liver cancer) have been treated in our hospital. The most important approaches used to uncover subclinical primary liver cancer were 1) the use of alpha-fetoprotein (AFP) monitoring in patients with liver disease; 2) the use of an AFP survey among high-risk area populations; and 3) X-ray examination of the diaphragm and sonography examination. The resectability rate of this series was 50%. The 3 and 5 year survival rates after hepatectomy were 62.5 and 57.1%, respectively. Hepatic arterial ligation combined with irradiation for the nonresectable cases had a better result. We discuss here the approaches and the diagnostic criteria of subclinical primary liver cancer.

Adult↗
PubMed1989

Inflammatory pseudotumor of the liver.

Since the first report of inflammatory pseudotumor of the liver in 1953, 12 cases have been reported in the literature. The authors report herein five cases of inflammatory pseudotumor of the liver in our hospital. These tumors were resected and the diagnosis confirmed pathologically. Three of the patients were male and two female. Four of these patients had been misdiagnosed as having primary liver cancer by sonography and CT scan. Our experience has shown that if the patient had a long clinical course, few clinical symptoms, good general condition, and a negative alpha-fetoprotein assay, if the tumor was well encapsulated and the liver presented without cirrhosis, the patients should be suspected of having inflammatory pseudotumor of liver. In this series, all five patients underwent hepatectomy. There were no complications after operation. Surgical resection should be considered as the main therapeutic method for inflammatory pseudotumor of liver. If the patient proves unsuitable for surgical treatment, steroid therapy should be the treatment of choice. In this paper 12 cases of inflammatory pseudotumor of liver reported in the literature are reviewed and discussed.

Adolescent↗
PubMed1988

Surgical management of soft tissue sarcomas, with an analysis of 313 cases.

From 1964 to 1978, 313 patients, 192 males and 121 females, with soft tissue sarcoma underwent surgery in our hospital. All patients have been followed up for over 5 years. One hundred ninety patients (60.7%) had recurrence of the tumor after previous surgery. The tumor was located in the head in 55 cases, in the trunk in 136, and in the extremities in 122 cases. Regional lymph node metastases occurred in 23 patients (7.4%). Fibrosarcoma, neurofibrosarcoma, and rhabdomyosarcoma were the types most commonly encountered in this series. The 5-year survival rate, according to the extent of surgical resection for 117 patients with local resection, was 47.9%; for 75 patients with wide local resection, 62.7%; for 43 patients with amputation, 18.6%; and for 78 patients with local resection combined with irradiation, 48.7%. For the whole series, the 5- and 10-year survival rates were 47.6% and 35.8%, respectively. The 5-year survival rates of stages I, II, III, and IV (TNM classification) were 60%, 46.9%, 40%, and 22.2%, respectively.

Adult↗
PubMed1988

Treatment of advanced malignancies with ifosfamide under protection with mesna.

Fifty-seven patients with advanced malignant tumours were treated with ifosfamide (Holoxan) and mesna (Uromitexan) in our department from November 1979 to December 1984. This series comprised eight cases of soft tissue sarcoma, nine cases of ovarian carcinoma, five cases of non-seminomatous testicular tumour, 11 cases of bronchogenic carcinoma, three cases of renal carcinoma, seven cases of non-Hodgkin's lymphoma, two cases of skeletal fibrosarcoma, two cases of breast carcinoma, one case each of Ewing's tumour, prostatic carcinoma, seminoma, plasma cell tumour, multiple myeloma, malignant teratoma, nasopharyngeal carcinoma, Wilms's tumour, neuroblastoma and mycosis fungoides. Out of these 57 cases, 53 were evaluable. There were five complete remissions and 20 partial remissions, corresponding to a total response rate of 47%. The overall median survival time (MST) of the 53 evaluable patients was 7.5 months. The responders had a longer survival time (MST 10 months) than the non-responders (MST 4.75 months) (p greater than 0.05). Analysis of the results according to sex, age, dosage of ifosfamide and degree of histological differentiation of the tumour cells failed to show any influence of these factors on the therapeutic results. The response rate to ifosfamide found in this study might be related to the histological origin of the tumours and to whether the primary tumours had been resected. The non-seminomatous testicular tumours, non-Hodgkin's lymphomas and ovarian carcinomas showed a high response rate. The response rate was higher in the group in which the primary tumour had been resected (61%) than in the non-resected group (12%) (except the non-Hodgkin's lymphoma). The side-effects of this regimen were moderate. Dyspepsia, nausea, vomiting, myelodepression, dizziness, and alopecia were common. Cystitis could be prevented nearly completely by concomitant administration of mesna, when given correctly, for preventing side-effects of ifosfamide on the urinary system (haemorrhagic cystitis, etc.).

Adult↗