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Biomedical subjects

J Prost

Publications and source records attributed to J Prost.

At least 91 records · Page 5Linked to original sources
PubMed1987

[Influence of protein malnutrition followed by a balanced refeeding on the synthesis level of pancreatic hydrolases in growing rats].

The intake of 5% gluten + casein (4:1, W:W) (PM) (E group) diet compared to 20% (16:4, W:W) (control group) caused an overall decreased synthesis. With the duration of PM, Tg2 and Chtg1 synthesis remained steady, while lipase synthesis decreased and amylase synthesis increased. At the beginning of refeeding (two days), in E group Tg2 and amylase synthesis was increased with rebound effect compared to controls, then decreased, Chtg1 synthesis was higher than control synthesis and remained constant, lipase synthesis was increased and reached the control value only after 9 days.

Animals↗
PubMed1984

[Effect of various environmental factors on the incidence of mastitis in cows and implementation of prevention programs].

The analysis carried out aimed at obtaining numerical data concerning the environmental conditions and finding about whether their improvement would contribute to control mastitis. Examinations of the environmental conditions were made seven times during the year, and mastitis was found in 2,088 cows of the black and white breed in 12 cowsheds of the state farms in the Szczecin district. The analysis resulted in gradual improvement of zoo sanitary conditions and in a decrease of mastitis cases. This correlation was proved statistically. A characteristic correlation was found between the sanitary state and infection intensity r = 0,948, between the sanitary state aNd irritation intensity of this gland r = 0,945. Moreover, the duration of udder dryness in cows was analysed. In 50,43% of the cows examined the period of dryness was short. In these cows the intensity of infection was higher (81,7%) than in cows with a normal period of dryness (33,5%).

Animal Husbandry↗
PubMed1984

[Effect of various environmental factors on the development of mastitis in cows and the implementation of a control program].

The analysis carried out aimed at obtaining numerical data concerning the environmental conditions and finding out whether their improvement would contribute to control mastitis. Examinations of the environmental conditions were made seven times during the year, and mastitis was found in 2,088 cows of the black and white breed in 12 cowsheds of the state farms in the Szczecin district. The analysis resulted in gradual improvement of zoo sanitary conditions and in a decrease of mastitis cases. This correlation was proved statistically. A characteristic correlation was found between the sanitary state and infection intensity r = 0,948, between the sanitary state aNd irritation intensity of this gland r = 0,945. Moreover, the duration of udder dryness in cows was analysed. In 50,43% of the cows examined the period of dryness was short. In these cows the intensity of infection was higher (81,7%) than in cows with a normal period of dryness (33,5%).

Animal Husbandry↗
PubMed1983

[Comparative study of the effects of 2 types of protein-energy malnutrition followed by a balanced refeeding, on the lipase, phospholipase A2 and amylase activities of the pancreas and pancreatic juice in the growing rat].

The intake of 2 p. 100 casein diet as only protein source decreased overall phospholipase A2 activity. Amylase activity was more affected than lipase one. The effects of intake of 5 p. 100 gluten diet as only protein source were less important than 2 p. 100 casein diet. Refeeding on 20 p. 100 or 15 p. 100 casein diet caused a considerable increase of phospholipase A2, lipase and amylase activities.

Amylases↗
PubMed1982

[Comparative study of the effects of 2 types of protein-energy malnutrition followed by a balanced realimentation on trypsin and chymotrypsin activity of the pancreas and the pancreatic juice of the rat].

The intake of 2 p. 100 casein or 5 p. 100 gluten diet as only protein source caused overall protein synthesis and immediately stopped the rat growth. Trypsin activity was less affected than chymotrypsin activity in pancreas. Refeeding on 20 p. 100 or 15 p. 100 casein diet caused a considerable increase of trypsin and chymotrypsin activities during the first week.

Animals↗
PubMed1980

[Effect of a protein-deficient diet (5 p. 100 gluten) and of balanced refeeding (15 p. 100 casein) on potential lipase, colipase-dependent lipase and phospholipase A2 activities. I. In the growing rat pancreas].

The intake of a 5 p. 100 gluten diet as the only protein source caused an overall protein synthesis deficiency that immediately stopped the rat growth. At first, the pancreas was less affected than the rest of the organism, but after 29 days of malnutrition, there was a 40 p. 100 decrease in body and pancreatic weight. The protein level of the deficient pancreas remained at that of the controls. Refeeding caused a considerable but temporary increase in that level after 24 h. The specific enzyme activities (AS) showed high variations from one day to the other, but only phospholipase A2 AS was significantly affected by the deficiency (--50 p. 100). Total lipase activities of the pancreas were reduced by 50 p. 100 and those of phospholipase A2 by 70 p. 100. Refeeding caused a considerable increase in lipase activities in 12 h and in phospholipase A2 activity in 48 h, but this process was brief and did not indicate complete recovery of those activities. After 27 days of refeeding, only phospholipase A2 showed good levels. Adding methionine to the deficient diet only changed the colipase level; this constitutes one more argument in favor of a distinct synthesis for this factor and for lipase.

Animals↗
PubMed1980

[Effect of a protein-deficient diet (5 p. 100 gluten) and of balanced refeeding (15 p. 100 casein) on potential lipase, colipase-dependent lipase and phospholipase A2 activities. II. In the pancreatic juice of the growing rat].

We experimented with a diet resembling a protein-deficient one eaten by man, having a low protein level and proteins of poor biological value. Malnutrition caused weight loss accompanied by an overall reduction of ingesta. However, the food intake, as compared to animal weight, was the same in the undernourished animals as in the controls. Bile and pancreatic juice outputs were not altered continuously and regularly but with the poor protein diet, they were lower. Refeeding a balanced diet caused these outputs to increase. When 0.6 p. 100 of methionine was added to the deficient diet, no significant difference was noted in the outputs of the rats eating the deficient diets. Enzyme activities in the juice and pancreas varied widely from one day to another during malnutrition and during refeeding. The mean values of specific activities, which masked these variations, showed that malnutrition did not significantly decrease the phospholipase A2 activity in the pancreatic juice, while potential lipase and colipase-dependent lipase activities declined. Refeeding temporarily stimulated specific phospholipase A2 and potential lipase activities the third day; colipase-dependent lipase activity was not activated. After 2 to 3 weeks of refeeding, the specific activities of the three enzymes were similar to the control values, but were no longer so at the end of refeeding. This would suggest a return to the normal, as shown by the oscillations around the control values. Protein malnutrition, as refeeding, did not in general cause the same changes in all the enzymes at the same time.

Animals↗
PubMed1978

[Adaptation of lipase and phospholipase A activities in pancreas and pancreatic juice in rats with diets rich in triglycerides and phospholipids].

Lipid rich diets containing about 20% of triglycerides or phospholipids given to Rats during 2 months were observed to increase lipase and phospholipase A2 activities in pancrease and pancreatic juice. The phospholipase and lipase activities are higher, respectively, on the phospholipid and triglyceride diet. Lower effects are observed after a 7-day administration of diet containing 40% of total lipid.

Animals↗
PubMed1978

[Effects of diets rich in triglycerides and phospholipids on lipase and phospholipase A activities in rat pancreatic juice and pancreas].

Lipase and phospholipase A activities were increased in the pancreas and pancreatic juice of rats ingesting during two months 20% phospholipids- or triglycerides-rich diets. The activity of lipase is higher with the triglycerides-rich diet and the phospholipase A activity is higher with the phospholipids-rich diet. After seven days of the experiment, these two enzymes activities were less increased with diet containing 40% lipids.

Animals↗
PubMed1978

[Effects of isoproteic and lipid rich diets on lipase, phospholipase A2, cholesterolesterase, trypsin and amylase activities in rat pancreatic juice and pancreas (author's transl)].

Isoproteic and lipid-rich diets containing about 15 or 20 % of sun flower triglyceride or soya phospholipids given to rats for 8 weeks were observed to increase lipase, phospholipase A2 and trypsin synthesis in the pancreas phospholipase A2 and trypsin synthesis in the pancreas and the activities of these enzymes in the pancreatic juice (Tables IV, V). Smaller effects are observed after 15 day administration of diets containing 40 % of triglycerids or phospholipids ; these diets decreased the ADN content of pancreas.

Amylases↗
PubMedDate not supplied

[Effect of fish protein on blood pressure, glycemia and plasma and urinary parameters in spontaneously hypertensive rats (SHR) and diabetic SHR].

Fish protein (FP) effect compared to casein (CAS) was studied on blood pressure (BP) evolution and glycemia in part, and on plasma lipid, angiotensine II and aldosterone concentrations and urinary aldosterone on the other hand, in SHR and in SHR with streptozotocin-induced diabetes (SHR-STZ). Two groups of rats (6 SHR and 6 SHR-STZ) were fed for 2 months diet containing 20% CAS or 20% FP. One month before sacrifice, diabetes was induced into one group of rats by a single intraperitonealy injection of streptozotocin (STZ). The results showed that FP diminished blood pressure (-9%) in SHR, compared with CAS. In contrast, FP enhanced significantly blood pressure in SHR-STZ vs SHR (p<0.01). There was no effect on glycemia with fish protein. FP compared to CAS involved a diminution (-41%) and (-17%) of total cholesterol and (-26%) and (-9%) of phospholipids in SHR and SHR-STZ, respectively. Moreover, a decrease of triacylglycerols (-21%) was noted in SHR-STZ with FP vs CAS. In SHR, plasma aldosterone and angiotensine II concentrations were reduced (-62%) and (-64%) and urinary aldosterone amounts were enhanced with FP compared to CAS (p<0.05). In SHR-STZ group, aldosterone value was fivefold lower in plasma and twofold higher in urine with FP compared to CAS. A significant enhancement of urinary aldosterone was noted in SHR-STZ vs SHR whatever diet-consumed. In conclusion, FP has a beneficial effect on blood pressure by modulating the hypertension markers i.e. plasma total cholesterol, angiotensine II and aldosterone, in SHR group, and on total cholesterol and triglycerids in SHR-STZ. FP reduces plasma aldosterone by its enhanced urinary excretion. It is necessary to specify the action mode of FP in order to propose it as nutritional complement in the degenerative diseases such as hypertension and diabetes.

Aldosterone↗
PubMedDate not supplied

[Gestational or essential hypertension in pregnant women limits the capacity to stock triglycerides by the placenta despite raised lipoprotein-lipase activity].

Placenta lipoprotein lipase (LPL) activity as well as serum VLDL and placenta lipids composition were determined in pregnant hypertensive women at term. 46 patients aged from 29 +/- 2 years with gravidic hypertension (HTA-G) and 38 patients with essential hypertension (HTA-E) aged 30 +/- 1 years were compared with 20 normotensive women aged 27 +/- 1 years. Serum triacylglycerols (TG) concentrations were 1.3-fold higher in the both hypertensive patients compared with controls. However, serum phospholipids (PL) and total cholesterol (TC) values were similar in the three groups. VLDL mass and their apolipoproteins, unesterified cholesterol (UC) and cholesteryl esters (CE) contents were significantly increased in hypertensive women compared with controls. In HTA-G and HTA-E patients, respectively. TG-VLDL concentrations were increased by +43% and +36% compared with those of controls (P < 0.01). In placenta, the values were lower 2.2- and 1.9-fold for TG, 2.8 and 2.5-fold for PL and two- and threefold for TC, in HTA-G and HTA-E patients than in controls. Placenta LPL activity was 2.7-fold higher in HTA-G and HTA-E patients compared with that of controls. In conclusion, although placenta LPL activity is higher it is not permit a decrease of serum TG-VLDL on the one hand, and an increase of placenta ability in TG storage on the other hand.

Adult↗
PubMedDate not supplied

[Fish proteins moderate triacylglycerols, activities of hepatic triacylglycerol lipase and tissue lipoprotein lipases in hypertensive and diabetic rats].

Fish proteins effect compared with casein was determined on triacylglycerols (TG) metabolism and activities of hepatic triacylglycerol lipase (HTGL) and tissue lipoprotein lipases (LPL), in SHR and diabetic SHR. Two groups of rats (n=24) were fed, 2 months, diet containing 20% casein (CAS) or 20% fish proteins (FP). One month before sacrifice, diabetes was induced in one group of rats by a single intraperitoneal injection of streptozotocin (STZ) (60 mg/kg of body weight). FP vs. CAS showed a significant decrease of TG concentrations in plasma (-41%) and liver (-66%), in SHR-STZ. VLDL-LDL mass, which represented the amount of apolipoproteins, TG, phospholipids (PL), unesterified cholesterol (UC), and cholesteryl esters (CE), decreased by 21 and 16% with FP vs. CAS, in SHR and SHR-STZ, respectively, and was concomitant with its low TG. Indeed, TG values was 1.35- and 1.82-fold lower in SHR and SHR-STZ, respectively. In HDL2, a TG diminution of 13% was noted, in SHR with FP vs. CAS. In SHR-STZ with FP, TG and PL were enhanced by 11% and 27%, respectively compared to SHR. HTGL activity decreased by 22% in SHR fed FP compared to those fed CAS. In SHR-STZ with CAS vsSHR, this activity was decreased by 21%. LPL activity in heart was enhanced by 19% in SHR-STZ vs. SHR with FP, when that of muscle was diminished 1.5-fold in SHR with FP vs. CAS and 1.8-fold in SHR-STZ vs. SHR with CAS. Adipose LPL activity was 1.36-fold higher in SHR with FP than CAS. In conclusion, it appears that fish proteins have a hypotriglyceridemic effect, which the mechanism can differ in SHR or SHR-STZ. It may be of interest to propose these fish proteins as lipid metabolism regulator in diseases with hypertriglyceridemia.

Animals↗
PubMedDate not supplied

Time course of changes in rat pancreatic synthesis rates and retention thresholds of four hydrolases during consumption of a low-protein followed by a balanced diet.

Synthesis rates of pancreatic amylase, trypsinogen 2 (Tg2), chymotrypsinogen 1 (Chtg1), and lipase, were measured in control rats fed a 20% protein diet for 46 days and in malnourished rats fed a 5% protein diet for 23 days (protein malnutrition) followed by refeeding a balanced diet for 23 days. In the control group, a progressive pancreatic maturation (namely an increase in hydrolase synthesis per gram of tissue) appeared with age. In the malnourished group, pancreas maturation of the four hydrolases was inhibited. Synthesis rates of the four hydrolases were reduced to a lesser extent from day 2 of protein malnutrition. With continued protein malnutrition, Tg2 synthesis rates remained steady, whereas the lipase synthesis rate continued to decrease and Chtg1 and amylase rates started to increase. As soon as refeeding was initiated, an important enhancement in synthesis was observed. The synthesis rates of Tg2, Chtg1, and amylase actually showed a rebound effect, then decreased with the refeeding time to reach control values, except for Chtg1, which remained higher than control values throughout the refeeding phase. Lipase synthesis rate rose more slowly and reached the control values only after 9 days of refeeding. The retention threshold (pancreas tissue versus pancreatic secretion) values showed that the storage levels were different from one hydrolase to the other and were variable with age in the control group, and with protein malnutrition and refeeding times in the malnourished group. In the control group, a preferential secretion of newly synthesized enzymes was observed in young rats, whereas with age, the proportion of newly synthesized hydrolases excreted decreased slowly.(ABSTRACT TRUNCATED AT 250 WORDS)

Amino Acids↗