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Biomedical subjects

J Pitha

Publications and source records attributed to J Pitha.

At least 91 records · Page 5Linked to original sources
PubMed1986

Muir-Torre syndrome. Histologic spectrum of sebaceous proliferations.

The sebaceous proliferations seen in patients with Muir-Torre syndrome are unique and difficult to classify. Having reviewed over 50 skin biopsies from five patients with Muir-Torre syndrome, we conclude that the primary proliferation involves the hair follicle and most often resembles either a keratoacanthoma or a sebaceous proliferation, but may have histologic features of both lesions. Unusual patterns seen in the sebaceous components may include solid basaloid sheets, mucinous areas, and convoluted glands.

Adenoma↗
PubMed1986

Characteristics of the beta-adrenoreceptor from neuronal and glial cells in primary cultures of rat brain.

The cellular characteristics of the beta-adrenoreceptor in glial and neuronal cells from the newborn rat brain were determined by (-)-[125I]iodocyanopindolol binding. In membranes from both cell types, the binding was saturable and from competition assays the potency series of (-)-isoproterenol greater than (-)-epinephrine = (-)-norepinephrine greater than (+)-isoproterenol was observed. 5'-Guanylyl-imidodiphosphate reduced the affinity of (-)-isoproterenol for the beta-adrenoreceptor from glial cells but had no effect on agonist affinity in neuronal cells. Chronic treatment of both cell types with (-)-isoproterenol reduced the receptor content and the capacity of the agonist to increase the cellular cyclic AMP content. However, the receptor recovery after chronic agonist treatment was faster in glial cells (72 h) than neuronal cells (120 h) and was blocked by cycloheximide. Treatment of both types with the irreversible beta-blocker bromoacetylalprenololmentane (2 microM) reduced the receptor content by 78% but no receptor recovery was observed for 120 h after the initial receptor loss. The data indicated that the majority of beta-adrenoreceptors in both cell types are the beta-1 subtype, but show some differences in receptor-agonist interactions. Furthermore, these CNS cells may be useful models for regulatory studies on the beta-adrenoreceptor.

Adrenergic beta-Agonists↗
PubMed1986

Lung beta-adrenoreceptor blockade affects perinatal surfactant release but not lung water.

We induced beta-adrenergic receptor blockade at 28 days gestation in the fetal rabbit with an irreversible beta-antagonist, bromace-tylalprenolomenthane (BrAlp). There was a marked decrease in concentration of available receptors in lung with increasing doses of BrAlp. BrAlp treatment decreased isoproterenol, but not prostaglandin, stimulated adenosine 3',5'-cyclic monophosphate (cAMP) generation in lung minces, and had no effect on activation of adenylate cyclase through non-beta-receptor-mediated components of the cyclase system in particulate preparations. Phospholipid recovery via lung lavage was significantly less from treated fetuses than from controls in groups delivered by cesarean section at 30 days (-31%) or vaginally at 31 days (-34%) and not allowed to air breathe. However, if fetuses from either group were allowed to air breathe, the difference was abolished. BrAlp treatment did not affect the phospholipid composition in lavage fluid, the rate of phosphatidylcholine synthesis, or tissue content of total or saturated phosphatidylcholine. Beta-adrenergic receptor blockade did not produce a significant change in lung water content either at or after birth regardless of the route of delivery. These data indicate that endogenous catecholamines play a role in surfactant secretion in both the fetal and newborn rabbit. We found no effects of BrAlp treatment on lung water, suggesting perhaps a less important role of endogenous catecholamines or that fewer receptors are required for this response than remained after treatment.

Alprenolol↗
PubMed1986

Differential recovery of beta adrenoreceptor antagonist and agonist high affinity binding sites in the guinea-pig lung after irreversible blockade.

The recovery of guinea-pig lung beta adrenoreceptors and beta adrenergic-mediated airway responsiveness was studied after irreversible receptor blockade with bromoacetylalprenololmentane (BAAM). The antagonist receptor concentration and those receptors able to form the agonist high affinity binding state were determined by (-)-[3H]dihydroalprenolol (DHA) and [3H]hydroxybenzylisoproterenol (HBI) binding, respectively. Airway responsiveness was measured by the ability of isoproterenol infusions to shift the histamine dose-response curve for airway constriction. Twenty-four hours after a single i.p. injection of BAAM (20 mg/kg), both [3H]DHA and [3H]HBI binding were reduced by 48 to 51% without changing the KD value for either ligand. In addition, there was a complete block of airway responsiveness to isoproterenol infusion at 1 microgram/kg/min although responsiveness similar to control values was observed at a higher infusion rate (10 micrograms/kg/min). Ninety-six hours after BAAM treatment, [3H]DHA binding had recovered to the control value whereas no recovery of [3H]HBI binding or of airway responsiveness was observed. By 144 hr after injection, both [3H]HBI binding and airway responsiveness had recovered to control values. The data indicate that after irreversible blockade, the total receptor population as measured by antagonists recovers relatively quickly followed by a slower recovery of those receptors able to form the agonist high affinity binding state that parallels the recovery of airway responsiveness.

Adrenergic beta-Agonists↗
PubMed1985

Irreversible inactivation of the beta-adrenoreceptor by a partial agonist. Evidence for selective loss of the agonist high affinity binding sites.

The catecholamine derivatives aminomenthylnorepinephrine (compound 1) and bromoacetylaminomenthylnorepinephrine (compound 2) were synthesized and their interaction with the rat lung beta-adrenoreceptor was characterized. Compared to (-)-isoproterenol, compounds 1 and 2 were 10 and 280 times less potent, respectively, at inhibiting (-)-[3H]dihydroalprenolol binding. At pH 7.4, all 3 compounds induced a loss of receptors (40-60%) which could be recovered by treatment with guanyl-5'-yl imidodiphosphate (Gpp(NH)p). However, at pH 8.1 Gpp(NH)p treatment did not recover those receptors lost by compound 2 only. The compound 2-induced receptor loss at pH 8.1 was time-dependent, prevented by propranolol but unaffected by Gpp(NH)p or after membrane heating at 50 degrees C which prevented the formation of the agonist high affinity binding state. Although, the maximal receptor loss as measured by [3H]dihydroalprenolol was 40-60%, more than 80% of the receptors were lost when measured by direct agonist binding, and the receptors left showed little agonist high affinity binding state formation. In rat reticulocyte membranes, compounds 1 and 2 stimulated adenylate cyclase activity with intrinsic activities of 0.55 and 0.31, respectively. However, at pH 8.1, compound 2 initially stimulated the enzyme followed by a blockade. These data indicated that both compounds 1 and 2 were partial beta-adrenoreceptor agonists and, at pH 8.1, compound 2 appeared to bind irreversibly only to those lung receptors able to form the agonist high affinity binding state. Furthermore, after irreversible binding, compound 2 appeared to act as an antagonist.

Adenylyl Cyclases↗
PubMed1985

Amorphous water-soluble derivatives of cyclodextrins: nontoxic dissolution enhancing excipients.

Dissolution properties of drugs may be improved by their conversion to an amorphous state or by complexation with cyclodextrins. The present report describes the preparation of cyclodextrin derivatives which are intrinsically amorphous, and water-soluble, and their use as complexation agents. Such derivatives were prepared by condensation of alpha-, beta-, or gamma-cyclodextrins with epoxides (propylene oxide, isobutylene oxide, epichlorohydrin, 1,4-butanediol diglycidyl ether). The condensation products effectively solubilized estradiol, progesterone, or testosterone in water; these solutions, upon freeze-drying, yielded solids which could be directly compressed to tablets which dissolve completely within minutes. Condensation products of cyclodextrins did not have any untoward or toxic effects when administered chronically to mice per os for a 16-week period.

Animals↗
PubMed1985

Alkylating beta-blockers: activity of isomeric bromoacetyl alprenolol menthanes.

An affinity label for beta-adrenoceptors, N-(bromoacetyl)-N'-[3-(o-allylphenoxy)-2-hydroxypropyl]-1,8-dia min o-p-menthane, has been extensively used in the form of a mixture of four isomers. In the present study, all four isomers were isolated, their structures elucidated, and their interactions with beta-adrenoceptors characterized. The isomer with the aromatic (pharmacophore) group on carbon 1 of p-menthane and with the Z configuration (Z-1) predominates in the mixture and has the highest affinity for beta-adrenoceptors of rat heart (KD = 3 X 10(-8) M) and lungs (KD = 2 X 10(-8) M). This isomer acts as a ligand that binds irreversibly at the drug binding site of the receptor (i.e., after treatment and extensive washing of the membrane preparation, the concentration of the receptors is decreased in a dose-dependent manner), while binding characteristics of the remaining receptors are not changed. The corresponding E diastereomer (E-1) also binds irreversibly to the drug binding site of the receptor. The isomer with the aromatic group on carbon 8 and the Z configuration (Z-8) modifies the receptor noticeably only at higher concentrations and then on a site apparently different from the drug-binding site, i.e., affinity of receptors after the treatment and washing is changed. The corresponding E diastereomer (E-8) modified both the drug-binding and alternative binding site. The results suggest that there is some flexibility in the conformation of the beta-adrenoceptor that enables pairs of ligands, differing by axial or equatorial positions of critical groups, to alkylate the receptor in an analogous manner.

Adrenergic beta-Antagonists↗
PubMed1985

Peripheral nerve changes following epineurial injection of saline and blood in rat sciatic nerve.

The effects of warm saline and heparinized autogeneic blood injection on a rat sciatic nerve were observed by histologic, histochemic, and CT planimetry. Demonstrable intraneural degenerative changes and epineurial cellular proliferation were present in both groups. Hemosiderin deposits were present in the epineurium and intraneurally only in the blood group. Epineurial saline injection produces nerve damage; therefore, its use for the purpose of neurolysis is not recommended. Further investigations are needed to study the effect of nonheparinized blood injection. The use of computerized planimetry in assessing quantitative nerve damage proved to be feasible.

Animals↗
PubMed1985

Relation of the hepatic and splenic microcirculations to the development of lesions in experimental amyloidosis.

By the use of a perfusion technique for identifying blood vessels, it was found that amyloid lesions in mice have a close relation to the underlying microcirculation. The earliest lesions develop about arteriolar capillaries. With the onset of the lesions, circulation of plasma not only continues through the affected vessels but also extends into the entire volume of the surrounding lesions. Progression of the lesions follows the underlying microcirculation, and there is a continuing presence of circulating proteins. Other observations, from tracer studies using labeled plasma proteins and studies of unfixed, frozen, tissue sections following saline extraction, indicate that much of the early amyloid lesions in these animals is circulating plasma. It would appear that the onset and site of formation of the lesions is determined by arteriolar capillary injury rather than by polymorphism of SAA proteins. Structural amyloid fibril proteins may gain access to the lesions by either entry from the circulation or local formation.

Amyloidosis↗
PubMed1984

The effect of bromoacetylalprenololmentane on rat atrial tension development and beta-adrenoreceptors.

In isolated rat atria, 1 microM bromoacetylalprenololmentane shifted the tension development curve for isoproterenol to the right of the control curve. After a 2 h washout period, no significant recovery of the isoproterenol concentration-response curve had occurred. Pretreatment of rat atrial membranes with 1 microM bromoacetylalprenololmentane resulted in an 83% decrease in the concentration of beta-adrenoceptors with no change in the KD value for [125I]iodocyanopindolol binding to the receptors left. The results indicated that bromoacetylalprenololmentane was an irreversible beta-blocker.

Alprenolol↗
PubMed1984

Digitonin derivatives of low toxicity: potential solubilizers for lipophilic compounds.

Digitoxin was modified by condensation with propylene oxide or with 1,4-butanediol diglycidyl ether in aqueous alkali, yielding products in which some of the CH2OH groups of digitonin were converted to CH2OCH2CHOHCH3 or to CH2OCH2CHOHCH2O(CH2)4OCH2CHOHCH2OH groups, respectively. These modified digitonins were very soluble in water and chloroform and effectively solubilized lipophilic compounds into aqueous solutions; e.g., 2 mg of vitamin A or 0.6 mg of cholecalciferol could be dissolved per 1 mL of 5% aqueous solutions of modified digitonins. Compared with the toxicity of digitonin (LD50 4 mg/kg iv), the toxicity of modified digitonin was greatly reduced: doses of 500 mg/kg by intravenous infusion were not lethal for mice.

Adjuvants, Pharmaceutic↗
PubMed1983

Decreased response with age of the cardiac catecholamine sensitive adenylate cyclase system.

The cardiac beta-adrenergic coupled adenylate cyclase system was examined in young and old male Wistar rats. The concentration of binding sites for (-)3H-DHA in membranes prepared from cardiac ventricles was 21.1 +/- 2.78 (SD) fmoles/mg protein in 3-4 month old rats (young rats) and 31.2 +/- 2.20 fmoles/mg protein in 24 month old rats (old rats). The dissociation constant, KD was 4.3 +/- 1.8 nM and 6.7 +/- 1.7 nM for young and old rats, respectively. Various compounds were used to study the characteristics of activation of adenylate cyclase in homogenates from cardiac ventricles. Basal adenylate cyclase was reduced 30% in old animals compared to young (6.1 pmoles/min/mg protein in 24 month vs. 8.6 pmoles/min/mg protein in 3-4 month). (-)Isoproterenol (10(-5) M) alone stimulated adenylate cyclase greater than two-fold in young rats (10.6 pmoles/min/mg protein above basal) and this stimulation was 34% lower in old animals. GppNHp (100 microM), fluoride (10 mM), and forskolin (100 microM) activation of adenylate cyclase above basal was reduced 38, 37, and 34%, respectively, in the old animals. No significant changes between the two groups were noted in the apparent affinity of GppNHp either alone or in the presence of (-)isoproterenol nor in the affinities of catecholamine agonists for activation of cyclase. These results suggest a reduction in the amount of functional regulatory protein or possibly cyclase in 24 month old rat ventricular tissue compared to 3-4 month old tissue. However, this data does not rule out the possibility of altered molecular interactions of a full complement of regulatory protein(s) with beta-adrenergic receptor and/or catalytic adenylate cyclase.

Adenylyl Cyclases↗
PubMed1983

Poly-L-methionine sulfoxide: a biologically inert analogue of dimethyl sulfoxide with solubilizing potency.

Poly-L-methionine sulfoxide is a water-soluble polymer containing the sulfoxide moiety. The preparation and radiolabeling of this polymer is described and its bioeffects are compared with those of dimethyl sulfoxide. Poly-L-methionine sulfoxide is similar to dimethyl sulfoxide in that it is a potent solubilizer of lipophilic compounds in water. Although the partition coefficient of poly-L-methionine sulfoxide in 1-octanol-water is only 20 times lower than that of dimethyl sulfoxide, it was found not to penetrate into intracellular spaces. In contrast to dimethyl sulfoxide, poly-L-methionine sulfoxide and L-methionine sulfoxide were found to be ineffective in inducing differentiation in murine erythroleukemia cells and inhibiting differentiation of avian neural crest cells, suggesting that compounds effective in these processes must have the ability to penetrate into cells or membrane proteins. Overall lack of bioactivity of poly-L-methionine sulfoxide, combined with low toxicity (2 g/kg, iv, in the mouse with no effect), makes this compound a suitable inert solubilizer and carrier for lipophilic drugs.

Animals↗
PubMed1983

Effect of a beta-adrenergic antagonist on blood pressure, heart rate and beta-adrenoceptors in turkey poults.

An irreversible beta-adrenergic blocker, bromoacetylalprenololmenthane (BAAM), was administered both peripherally and centrally to turkey poults, Meleagris gallopavo. Peripheral administration of BAAM (60 mg/kg body weight) effected a significant reduction in blood pressure and heart rate. Twenty minutes postinjection, mean blood pressure and heart rate were reduced 34.5 and 24.2%, respectively. Two days later, mean blood pressure values remained significantly depressed at 17.3% below preinjection determinations. Biochemical analysis of heart tissue following peripheral (intraperitoneal) injections of BAAM (60 mg/kg body weight) showed a significant decrease in beta-adrenergic receptors (BAR). Little or no change in the number of BAR in brain tissue was observed. Central (intraventricular) administration of BAAM (0.72 mg/g brain weight) resulted in no change in mean blood pressure or heart rate during a 20 min postinjection period. Biochemical analysis of heart tissue following central injections of BAAM showed little or no change in the number of BAR. There was, however, a significant decrease in the number of BAR in brain tissue.

Adrenergic beta-Antagonists↗