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Biomedical subjects

J Pillot

Publications and source records attributed to J Pillot.

At least 163 records · Page 9Linked to original sources
PubMed1976

Optimal conditions for elution of hepatitis B antigen after absorption onto colloidal silica.

Hepatitis B surface antigen (HBSAg) adsorbed from sera onto colloidal silica could be completely eluted through the use of 0.25% sodium deoxycholate in 0.01 M borax, pH 9.3, at 56 degrees C. The HBSAg recovered in the eluate represented 100% of that present in the original serum, and it was contaminated by only trace amounts of serum proteins (in decreasing amounts: beta-lipoprotein, immunoglobulin G, albumin). This preliminary step greatly facilitates purification of large amounts of HBSAg and provides small volumes of highly concentrated material for subsequent purification by density gradient centrifugation.

Absorption↗
PubMed1976

Lack of antibody activity directed against the most common human bacteria in human myeloma protein.

The fact that sera of germ-free animals contain very little Ig suggests that most Ig probably correspond to antibacterial antibodies. We have detected an antibacterial activity against 40 bacterial strains, pathogenic and nonpathogenic, in 85 M-component of human origin. This work has been conducted by indirect immunofluorescence withipure labeled antibodies. The reasons for which indirect immunofluorescence seems to be the best technique for this kind of research are discussed. No activity whatsoever could be detected. The reasons of this lack of activity remain to be explained.

Antibodies, Bacterial↗
PubMed1975

[Circulating immune complexes in man; precipitation of immune complexes with polyethylene glycol and their characterization by Clq bound to immunoglobulins].

Use of an experimental model consisting of purified tetanal toxoid and sera of patients vaccinated with this antigen reveals that immunodiffusion characterisation of C1q bound to immune complexes (IC) precipitating with 2,5% PEG (6000) represents a very sensitive means of detection of such IC. The majority of sera of patients with disorders in which IC can be suspected gives positive reactions. Precipitates contain IgG and IgM bound to C1q and rheumatoid factor is constantly detected. The reaction is positive among 10% of healthy patients: the possibility of circulating IC free from any pathological cases is under study.

Antigen-Antibody Complex↗
PubMed1975

[Serodiagnosis of human brucellosis].

On the basis of the serological study of 1000 sera obtained from patients suspected of suffering from brucellosis, the authors emphasis the theoretical data which confer upon this complement fixation reaction a greater reliability than the agglutination reaction. Using an appropriate antigenic preparation, the complement fixation reaction indeed proves to be of greater interest than the classical Wright sero-agglutination test in the diagnosis of brucellosis.

Antibodies, Bacterial↗
PubMed1975

The problem of purification of HBs antigen.

Purification techniques utilized until now do not allow to obtain an HBs antigen totally free from one or more protein constituents of the serum. The authors describe the method which has given the best results; this method combines the utilization of silicate, Sephadex G200 and ultracentrifugation. The authors then determined the nature of the relations between HbsAg and the serum proteins. They have perfected a screening technique allowing to detect the immune complexes by means of a tetanus antigen-tetanus antitoxin model. Sixty-nine patients with viral hepatitis A and B were tested. It was observed that the immune complexes are in relation with the IgM which are themselves correlated with the rheumatoid factor: the mu chains found in purified HBsAg could correspond to the rheumatoid factor reacting with the Fc of IgG bound to HBsAg.

Antigen-Antibody Complex↗