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Biomedical subjects

J Peters

Publications and source records attributed to J Peters.

At least 631 records · Page 35Linked to original sources
PubMed1975

Genetic counseling.

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Adaptation, Psychological↗
PubMed1974

Fate of corticotrophins in an isolated adrenal-cell bioassay and decrease of peptide breakdown by cell purification.

1. The fate of corticotrophins in a trypsin-dispersed rat adrenal-cell assay system was investigated with a view to establishing whether differences in the rate of inactivation might contribute to potency differences observed between analogues. 2. Corticotrophin-(1-24)-tetracosapeptide and to a lesser extent synthetic 1-39 corticotrophins were found to be inactivated during incubation with cell suspension. 3. Peptide fragments were isolated by using [[(3)H(2)]Tyr(23)]corticotrophin-(1-24)- tetracosapeptide as a marker. The fragments indicate a peptidase with a predominantly tryptic specificity. 4. The peptidase is present in the extracellular fluid and is released from cells when they are damaged. 5. Cells were fractionated on an albumin gradient. Cells from the zona fasciculata and the zona intermedia or reticularis were present in fractions which produced fluorogenic steroids in response to corticotrophin. 6. Purification of the cells by centrifugation through albumin decreased degradation by peptidases, so that if the assay is carried out with a dilute suspension of purified cells peptide breakdown should not affect the observed potencies of adrenocorticotrophin analogues. 7. No binding of [[(3)H(2)]Tyr(23)]corticotrophin-(1-24)- tetracosapeptide to cells could be detected at low concentrations of the peptide. This indicated that less than 120 receptors/cell are occupied during stimulation by a dose that would elicit approx. 80% of the maximal response.

Adrenal Glands↗
PubMed1971

Intracellular enzymes and protein synthesis in rabbit skin after thermal injury.

1. The concentrations of several intracellular enzymes in rabbit skin have been measured 5 min, 2, 6 and 24 h after thermal injury.2. At 5 min and 2 h after a burn (60 degrees C for 1 min) there was a significant fall in the enzyme activities whereas at 6 h their activities were higher than control.3. It appears that the increase in enzyme concentrations in the lymph during the first few hours after thermal injury is associated with a fall in the enzyme concentrations in the tissues and therefore might be the result of leakage of enzyme from storage sites in the injured cells.4. The second increase in enzyme concentrations in the lymph which has been observed 6-18 h after thermal injury occurs at a time when there is also an increase in the enzyme concentrations in the tissue.5. It seems unlikely that these increased activities are due to new synthesis since there was no apparent correlation between tissue enzyme concentrations and protein synthetic activity, and the changes still occurred after administration of cycloheximide.6. There was a change in the LDH isoenzyme pattern after injury towards a predominance of LDH-1. This change did not occur immediately after the burn, but was present at 2 and 6 h, and returned to normal 24 h later.

Acid Phosphatase↗