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Biomedical subjects

J Peters

Publications and source records attributed to J Peters.

At least 595 records · Page 33Linked to original sources
PubMed1980

Linkage analyses of murine immunoglobulin heavy chain and serum prealbumin genes establish their location on chromosome 12 proximal to the T (5;12) 31H breakpoint in band 12F1.

Analysis of backcross mice carrying the Harwell translocation T(5;12)31H has led to the definitive localization of the immunoglobulin heavy chain gene cluster. Both Igh-1 and Pre-1 loci were found to segregate in tight linkage with the chromosomal markers 5(12) and 12(5), which define the balanced translocation T(5;12)31H. Additional data establish the location of these genes at the telomeric end of chromosome 12. That both loci are proximal to the chromosomal breakpoint in band 12F1 is shown by the phenotypes of segregants aneuploid for the presence or absence of the small marker 5(12). The order of loci inferred from a single recovered recombinant is: centromere-Igh-1-Pre-1-T(5;12)31H.

Animals↗
PubMed1980

S-adenosylmethionine levels and protein methylation during morphogenesis of Mucor racemosus.

The intracellular level of S-adenosylmethionine increased as the yeast-phase cells of Mucor racemosus were induced to convert to hyphae. This increase correlated well with time course of the conversion in cell type and was independent of the metabolic changes caused by the shift to aerobic conditions. There was no significant change in the intracellular level of spermidine, a polyamine synthesized from putrescine and the propylamine group of S-adenosylmethionine. Spermine was not detected. An examination of protein methylation revealed an increase in the methylation of total protein during the shift in cell type and possible qualitative as well as quantitative changes in specific base proteins.

Fungal Proteins↗
PubMed1980

Evaluation of scoring system of the chest radiograph in cystic fibrosis: a collaborative study.

A collaborative study including 16 cystic fibrosis centers, 22 observers, and more than 1,700 independent observations was undertaken to validate the reproducibility of a scoring system for evaluation of chest radiographs of patients with cystic fibrosis. Five large and 10 small centers were randomly selected by the project statistician. A total of 40 pairs (frontal and lateral) of chest radiographs representing varying degrees of severity was selected by a physician not involved in scoring. Duplicates of these films, identified only by code number, were scored independently by pediatric clinicians (small centers) and by both clinicians and radiologists (large centers). Data collection was complete for all observers. Analysis of the data confirms a moderately high degree of reproducibility by and among observers and indicates that this scoring system could be used in the assessment of patients with cystic fibrosis.

Alabama↗
PubMed1980

[Indication and results of radiation therapy in the treatment of astrocytomas (author's transl)].

The course of 77 patients with well-differentiated or anaplastic astrocytoma after treatment with resection and radiotherapy or radiotherapy after biopsy during the years 1954--1978 at the University hospital at Zürich is analyzed. The ten-year survival rate in patients with well-differentiated astrocytoma is about 30%. About 15% of the patients with anaplastic astrocytoma are living after 5 years, but there is no 10-year survivor in this group. Patients with mixed glioma (astrocytoma plus oligodendroglioma) show the same survival rate as patients with well-differentiated astrocytoma. Analysis of tumor dose and the size of the irradiated volume shows only in patients with anaplastic astrocytoma a trend to better survival rate after doses of at least 5000 rd in 5 weeks and irradiation fields larger than 200 cm2. In accordance with published reports, the survival rate in patients with gemistocytic astrocytoma is lower than in the other cases.

Adolescent↗
PubMed1979

[Epidemiology of hepatitis A: serological study among a French population (author's transl)].

To investigate the prevalence and distribution of antibodies to hepatitis A antigen (anti HAV Ab) we tested 848 French people living mainly but not exclusively in Franche-Comté (East of France). In Franche-Comté, 76 per cent of all the subjects studied were found to be anti-Hav ab positive, as determined by specific radio-immuno-assay. The rate of antibody detection was correlated with age; the prevalence was low (38 per cent) in childhood and adolescence, gradually increasing throught adulthood, and reaching its peak level (greater than 95 per cent) in people aged 40 and above. Anti-HAV Ab was detected more frequently in farmers (89 per cent), factory workers (87 per cent) and office employees (80 per cent) than in hospital personnel (53 per cent). Socioeconomic differences were found to be important in young people but tended to disappear at about 40 years of age. No difference was found in the prevalence of anti-HAV Ab between national servicemen from Franche-Comté and those from other regions. In 3 per cent of cases anti-HBs Ab was associated with the presence of anti HAV Ab. The subject possessing anti HBS Ab had the same prevalence of anti-HAV Ab (75 per cent) as those without (76 per cent).

Adolescent↗
PubMed1979

Morphology-associated expression nicotinamide adenine dinucleotide-dependent glutamate dehydrogenase in Mucorracemosus.

The in vivo regulation of glutamate dehydrogenase (GDH) was studied in Mucor racemosus as a function of nutritional conditions and morphological state. Both nicotinamide adenine dinucleotide (NAD) and nicotinamide adenine dinucleotide phosphate (NADP)-dependent GDH activities were found. The effect of carbon and nitrogen source on the specific activity of the NAD-dependent GDH suggests that its role is primarily catabolic. The NAD-dependent activity was generally an order of magnitude greater in mycelial cells than in yeast-phase cells grown on the same medium. During yeast-to-hyphal morphogenesis the increase in NAD-dependent activity preceded the appearance of hyphal cells both under aerobic and anaerobic conditions. Exogenous dibutyryl-cyclic AMP prevented the increase in NAD-dependent GDH concomitantly with the suppression of morphological differentiation. The NADP-dependent activity did not change appreciably during morphogenesis.

Bucladesine↗
PubMed1979

The importance of femoral rotation in chondromalacia patellae as shown by serial radiography.

A new radiological technique is presented in which serial axial radiographs of the patellofemoral joints are taken under conditions in which the muscles about the knee and hip are contracted in a manner similar to that during weight-bearing. A form of analysis has been developed whereby patellar rotation can be measured in two planes and femoral rotation about its long axis inferred. A population of asymptomatic adults and children was investigated in this way and their results (regarded as normal) compared with those in fifteen children with idiopathic chondromalacia patellae. In the normal child the femur rotates medially with the onset of muscle activity; by contrast the children with chondromalacia show a reversal of this mechanism.

Adolescent↗
PubMed1978

Genetic variation, cellular distribution and ontogeny of sorbitol dehydrogenase and alcohol dehydrogenase isozymes in male reproductive tissues of the mouse.

Cellulose acetate zymograms of alcohol dehydrogenase (ADH) and sorbitol dehydrogenase (SDH) extracted from male reproductive tissues of inbred mice were examined. ADH isozymes were differentially distributed in these tissues of C3H/He mice; ADH-B2 was observed in all tissues and testis cellular preparations examined; ADH-C2 was localized predominantly in the epididymis but was also present in the seminal vesicles, coagulating gland, and prostate gland. SDH was broadly distributed in these tissues but exhibited highest activities in the seminal vesicles, coagulating glands, and germinal cells of mature testes. Genetic variants for ADH-C2 and SDH provided evidence for (1) the identity of a second form of SDH in epididymis with ADH-C2; (2) the genetic identity of kidney, seminal vesicle, and testis SDH; and (3) the gentic identity of stomach and epididymal ADH-C2. Developmental changes in testis and epididymal ADH isozymes during maturation were examined. ADH-C2 appeared in the mature epididymis whereas ADH-B2 exhibited no major changes in activity in testis and epididymis during development.

Alcohol Oxidoreductases↗
PubMed1978

Esterases of Mus musculus: substrate and inhibition characteristics, new isozymes, and homologies with man.

A wide range of fluorogenic and naphthol esters has been tested as substrates for mouse esterases. New esterases have been identified in liver and kidney extracts with palmityl, oleyl, and elaidyl esters. From substrate, inhibition, and molecular weight studies, three homologies between human and mouse esterases are suggested. A new allele at Es-6 is also described.

4-Nitrophenylphosphatase↗
PubMed1978

Biochemical genetics of a new glucosephosphate isomerase allele (Gpi-1c) from wild mice.

We have found a new allele at the structural locus for glucosephosphate isomerase (called Gpi-1c) in a population of wild mice. The Gpi-1c allele codes for an enzyme of greater cathodal electrophoretic mobility than either the Gpi-1a or Gpi-1b alleles found in the wild and in the SM/J and C57BL/6J inbred strains. Mice homozygous for Gpi-1c have erythrocyte enzyme activity reduced to 33% of normal levels, altered pH profile, lowered heat stability, and normal Km's when compared with SM/J and C57BL/6J mice. The activity of the enzyme in brain, liver, and kidney is not so markedly lowered, although the electrophoretic mobility, pH profile, and heat stability are altered in these tissues. Deficiencies of erythrocyte glucosephosphate isomerase in man, to this level, can cause severe hemolytic anemia. Homozygotes for Gpi-1c show only mild hematological symptoms. The frequency of Gpi-1c in wild populations of mice is discussed and the occurrence of a further rare allele Gpi-1d is reported.

Alleles↗
PubMed1978

[Application of a new method for the calculation and description of the resistance of microbiological indicators. I. Testing of several common microbiological sterilization indicators (author's transl)].

The method described by SPICHER and PETERS (1975) for the calculation and description of the resistance of microbiological indicators was tested. As test objects served spore-containing earth according to DIN 58946, Attest indicators (3 M Company, Minnesota) and Oxoid Spore Strips (Oxoid Ltd., London). The tests were performed not only for different batches of indicators but also for preparations of different age. After application of steam (120 degrees C), the indicators were examined for the presence of surviving germs capable of multiplication. When plotting the frequency of indicators with surviving germs (q) against the duration of steam action, S-shaped curves were obtained as expected. By altering the scale of the ordinate (y = lg (-ln(1 - q))), the S-shaped curves could be transformed into straight lines. Thus, the experimentally established paired values could be used for a calculation of regression. This method of calculation proved to be suitable in all cases studied. By indicating the position and the slope of these straight regression lines, the resistance of microbiological indicators can be exactly described (cf. Table 2). This method is applicable not only to indicators containing culture spores but also for native spore-containing earth. The indicators examined differed in their resistance and stability. Seven out of eight batches of Attest indicators (cf Figs. 1 and 2 and Table 1) fulfilled the requirements of DIN 58946, Part 4, for the resistance of bio-indicators for steam sterilization. One of the batches had a slightly higher resistance. The Attest indicators tested were of good stability (see Fig. 1 and Table 1). Where surviving germs were present on the indicators after treatment by steam, their growth was recognizable, in 99% of cases, already after incubation of the cultures for 24 hours. Only two batches of Oxoid Spore Strips were available for testing. One batch was of a higher resistance than required by DIN 58946. The second batch was slightly above the lower limit of the permissible range (see Fig. 3). During storage for 12 months, the resistance of both batches was reduced by 3--4 min. Where the indicators exhibited surviving germs after treatment by steam, growth was recognizable in 87% of the cases after incubation for 24 hours, while for the other indicators, incubation for 48 hours was necessary. The experiments confirmed the good stability of native spore-containing earth (see Fig. 5). Within 4--5 years, the steam resistance of the preparations decreased only by 3--4 min.

Bacteriological Techniques↗