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Biomedical subjects

J Paul

Publications and source records attributed to J Paul.

At least 253 records · Page 14Linked to original sources
PubMed1979

The affinity of concanavalin A and wheat germ agglutinin for components of the muscle spindle.

Sections through the soleus muscle of the rat were incubated with concanavalin A (Con A) or wheat germ agglutinin (WGA) conjugated to fluorescein isothiocyanate. Binding of these lectins to structures which comprise the muscle spindle was studied by fluorescence microscopy. The distribution of the lectins was heaviest in the outer capsule of the spindle and at the surface of intrafusal muscle fibres. The periaxial space in the equatorial region of spindles was unlabelled except in the immediate vicinity of the axial bundle. Binding by Con A was more extensive than by WGA, suggesting that more glucopyranoside units are accessible within the muscle spindle than are those of N-acetylglucosamine.

Animals↗
PubMed1979

Changes in nuclear and polysomal polyadenylated RNA sequences during rat-liver regeneration.

Nuclear and polysomal polyadenylated RNA populations of normal and 16 hour regenerating rat liver have been compared by mRNA-cDNA hybridisations and by unique DNA saturation experiments. It was found that nuclear polyadenylated RNA hybridises to 6.8% of unique DNA in both normal and 16 hour regenerating rat liver. However, cross-hybridisation experiments using cDNA have shown that 10-15% by weight of nuclear polyadenylated RNA sequences are specific to 16 hour regenerating rat-liver. Since both unique DNA and cDNA hybridisation have shown that normal and 16 hour regenerating rat-liver polysomal polyadenylated RNA populations are qualitatively very similar sequences specific to 16 hour regenerating rat-liver nuclear polyadenylated RNA are nucleus confined. Polysomal RNA sequences which were abundant in normal rat-liver have become less abundant in regenerating rat liver.

Animals↗
PubMed1979

The Friend virus genome: partial characterization of a complete DNA copy.

A complementary DNA probe has been prepared from the Friend murine erythroleukaemia virus complex released by Friend cells (FV cDNAD-) and Friend cells induced to differentiate (FV cDNAD+). Molecular hybridization analysis shows that: (a) FV cDNAD+ is close to being a complete copy of the virus genome and the distribution of sequences is uniform with respect to their distribution in the Friend virus genome. (b) Hybridization of 70S RNA from the cloned helper virus to the total FVc DNAD+ probe demonstrates that a large proportion of the cDNA is specific to the transforming spleen focus forming virus. (c) Hybridization of the probe to normal and transformed cell DNA shows that there are about seven Friend virus related genes in normal DNA and almost twice this amount in transformed cell DNA. A significant minor proportion (20%) of the cDNA probe anneals only to virus related sequences in the transformed cell DNA. (d) An analysis of the kinetics of annealing of the cDNA to an excess template RNA shows that the minimum base sequence complexity of the Friend virus complex is 4 x 10(6). (e) An analysis of the cross hybridization between FV cDNAD+ and 60 to 70S RNA isolated from virus released by uninduced and induced cells shows that the genome of the induced and uninduced Friend virus is almost identical.

Animals↗
PubMed1979

Self-ownership, abortion and infanticide.

Doctors have been placed in an anomalous position by abortion laws which sanction the termination of a fetus while in a woman's womb, yet call it murder when a physician attempts to end the life of a fetus which has somehow survived such a procedure. This predicament, the doctors' dilemma, can be resolved by adopting a strategy which posits the right to ownership of one's own body for human beings. Such an approach will generate a consistent policy prescription, one that sanctions the right of all pregnant women to abortions, yet grants the fetus, after it becomes viable as a potentially independent person, a right to its own body. The doctors' dilemma is surmounted, then, by requiring that abortions of viable fetuses be performed in a manner that will produce a live delivery. Hence, infanticide and termination of viable fetuses are proscribed.

Aborted Fetus↗
PubMed1978

Structure of recombinant plasmids containing synthetic human foetal globin gene sequences.

In vitro synthesized duplex DNA complementary to human foetal globin messenger RNA was integrated into bacterial plasmids and amplified by transformation of Escherichia coli. Recombinants carrying globin DNA were identified by hybridization of foetal globin messenger RNA to bacterial DNA in situ and by liquid hybridization of purified plasmids to specific globin complementary DNA probes. Heteroduplex mapping revealed either a simple insertion loop at the position of the EcoRI site of the parental plasmid DNA. We provide evidence to suggest that these deletions are the result of a site-specific nicking activity of the EcoRI preparations used in the formation of recombinant plasmids.

DNA Restriction Enzymes↗
PubMed1978

Recombinant plasmids containing Xenopus laevis globin structural genes derived from complementary DNA.

Details are presented of the in vitro synthesis of double-stranded DNA complementary to purified Xenopus globin messenger RNA, using a combination of reverse transcriptase, fragment 'A' of E. coli DNA polymerase 1 and S1 endonuclease. After selection of duplex DNA molecules approaching the length of Xenopus globin messenger RNA by sedimentation of the DNA through neutral sucrose gradients, the 3'-OH termini of the synthetic globin gene sequences were extended with short tracts of oligo dGMP using terminal transferase. This material was integrated into oligo dCMP-extended linear pCR1 plasmid DNA and amplified by transfection of E. coli. Plasmids carrying globin sequences were identified by hybridization of 32P-labelled globin mRNA to total cellular DNA in situ, by hybridization of purified plasmids to globin cDNA in solution, by analysis of recombinant DNA on polyacrylamide and agarose gels, and by heteroduplex mapping. The results show that extensive DNA copies of Xenopus globin mRNA have been integrated into recombinant plasmids.

Animals↗
PubMed1978

Urinary tract abnormalities presenting as a result of blunt abdominal trauma.

Fourteen patients with unsuspected urinary tract abnormalities had their disorders diagnosed at the time of their evaluation for blunt abdominal trauma. The trauma was generally inconsequential but all of the patients had gross or microscopic hematuria. The diagnosis could be made by intravenous pyelography or physical examination in all of these patients, although in some patients the abnormality was not evident until the patient had an arteriogram. These disorders are usually detected in children, but 12 of our 14 patients were 19 years of age or older. The importance of the urogram for all patients with hematuria and minor abdominal trauma is emphasized by this group of patients.

Abdominal Injuries↗
PubMed1978

[The problem of documentation in pedodontics].

The efficiency of paedodontics increases with the number of children involved in regular dental supervision. This requires complete and reliable information about the individual factors, especially dental development, at any consultation (whether mass examination or treatment). The current methods of documentation are often inadequate to this purpose. For this reason, the author presents a method of documentation by means of key-word cards that essentially meets the demands to be made on a treatment chart.

Child↗
PubMed1978

Mechanism of glycoconjugates biosynthesis in the vascular wall with external circular constraint.

Extrinsic local constraints, realised on dogs aorta are responsible for parietal lesions in the form of thickening of the arterial wall. Histological changes in the intima and media are characteristic of processes of degeneration destruction and cellular regeneration, superposed on sub-endothelial proliferation accompained by partial or total destruction of endothelial cells in the intimal layer. In addition to histological changes, there were perturbation in enzymatic activities of glycosyltransferases responsible for macromolecular constructions in the intima. Microsomic enzymes studied were: N-acetyl-glucosaminyl-transferase, sialyl-transferase and N-acetyl-galactosaminyl-transferase, and two glycosyl-transferases located in the soluble cytoplasmic phase: fucosyl and xylosyl-transferase. The changes observed were different according to the position of the sugars in the glycoprotein chains. For example the variation of fucosyl-transferase activity is significant: it disappears in group where the sialyl-transferase activity is enhanced. The transfer of xylose is decreased. Finally, changes observed in hexosaminyl-transferase activities were parallel.

Animals↗