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Biomedical subjects

J Pattison

Publications and source records attributed to J Pattison.

At least 19 recordsLinked to original sources
PubMed1999

Laboratory evaluation of a compomer and a resin-modified glass ionomer cement for orthodontic bonding.

The mean shear debonding force of stainless steel orthodontic brackets with microetched bases bonded with either a compomer or a resin-modified glass ionomer cement was assessed. In addition, the amount of cement remaining on the enamel surface following bracket removal was evaluated. Finally, survival time of orthodontic brackets bonded with these materials was assessed following simulated mechanical stress in a ball mill. Debonding force and survival time data were compared with those obtained for brackets bonded with a chemically cured resin adhesive, a light-cured resin adhesive, and a conventional glass ionomer cement. There were no significant differences in mean shear debonding force of brackets bonded with the compomer, resin-modified glass ionomer, chemically cured resin adhesive, or the light-cured resin adhesive. Brackets bonded with a conventional glass ionomer cement had a significantly lower mean shear debonding force than that recorded for the other materials. The Adhesive Remnant Index (ARI) mode score indicated that significantly less cement remained on the enamel following debonding of brackets cemented with resin-modified or conventional glass ionomers compared with other adhesives. The median survival time for brackets cemented with the compomer, resin-modified glass ionomer, chemically cured resin, or light-cured resin were significantly longer than for brackets cemented with conventional glass ionomer. The compomer and the resin-modified glass ionomer adhesive appear to offer viable alternatives to the more commonly used resin adhesives for bracket bonding.

Acid Etching, Dental↗
PubMed1999

Off-axis x-ray spectra: a comparison of Monte Carlo simulated and computed x-ray spectra with measured spectra.

The off-axis x-ray spectra from a constant potential x-ray generator were measured with a high purity germanium spectrometer cooled to liquid nitrogen temperature. The measured spectra were compared with off-axis x-ray spectra calculated using a code based on the semiempirical model developed by Tucker et al. and Monte Carlo simulated x-ray spectra using the EGS4 code system. In this study, both the Tucker model, and the EGS4 code system, were found to produce off-axis bremsstrahlung x-ray spectra which agreed well with the spectra measured at three emerging angles. In the measured and the EGS4 generated spectra the total K-characteristic peaks were in increasing order, as observed in the anode to cathode direction, whereas the Tucker model produced maximum total K-characteristic peaks at the 6 degrees anode side, and lesser amounts at the central axis and the 6 degrees cathode side. Large differences in the total K-characteristic lines is seen among the three different methods. The EGS4 code system was able to produce x-ray spectra for a combination of target materials.

Computer Simulation↗
PubMed1998

Phosphate permeases of Saccharomyces cerevisiae.

The PHO84 and PHO89 genes of Saccharomyces cerevisiae encode two high-affinity phosphate cotransporters of the plasma membrane. Hydropathy analysis suggests a secondary structure arrangements of the proteins in 12 transmembrane domains. The derepressible Pho84 and Pho89 transporters appear to have characteristic similarities with the phosphate transporters of Neurospora crassa. The Pho84 protein catalyzes a proton-coupled phosphate transport at acidic pH, while the Pho89 protein catalyzes a sodium-dependent phosphate uptake at alkaline pH. The Pho84 transporter can be stably overproduced in the cytoplasmic membrane of Escherichia coli, purified and reconstituted in a functional state into proteoliposomes.

Amino Acid Sequence↗
PubMed1998

A 5-year clinical review of bond failure with a light-cured resin adhesive.

The purpose of this study was to investigate the time to first failure of stainless steel orthodontic brackets (Ormco Corp, Glendora, Calif) bonded with a light-cured resin adhesive (Transbond, 3M Unitek, Monrovia, Calif) and assess whether time to failure was related to the patient's age at the start of treatment or sex, the proficiency of the individual placing the brackets, or the presenting malocclusion. Data on 548 patients with 7118 bonded brackets were analyzed. Survival analysis was carried out on a single bracket per patient. In each patient, the first bracket to fail was studied. In cases where no brackets failed, the bracket with the shortest follow-up time was analyzed and handled as a censored observation in the formal analysis. Median time until first bracket failure was 442 days, and an overall failure rate of 6% was recorded. There were no significant differences in time to first failure of brackets with respect to the sex or age of the patient at the start of treatment (p = 0.168, and p = 0.261, respectively), operator proficiency in placing brackets (p = 0.189), or presenting malocclusion (p = 0.052). Performance of brackets bonded with Transbond does not appear to vary significantly according to the variables examined.

Adolescent↗
PubMed1998

Diagnostic x-ray spectra: a comparison of spectra generated by different computational methods with a measured spectrum.

A number of computer codes, developed using semi-empirical models, are available to compute x-ray spectra from a tungsten target for different tube parameters. In this study x-ray spectra measured with a high-purity germanium detector are compared with those computed using the empirical models and previously published measured data. The computer codes used to generate the spectra are based on models proposed by Birch et al. and Tucker et al. The measured x-ray spectra agreed well with the computed x-ray spectra using the model of Tucker et al. whereas the model of Birch et al. produced a "harder" x-ray spectrum compared to the measured spectra. Our measured x-ray spectra compared well with the previously published measured spectral data of Fewell et al.

Americium↗
PubMed1998

The effect on dose to computed tomography phantoms of varying the theoretical x-ray spectrum: a comparison of four diagnostic x-ray spectrum calculating codes.

Theoretical x-ray spectra calculated by four different codes for the same tube parameters are compared by calculating and measuring doses to computed tomography (CT) body and head phantoms. The effect on the 120 kV spectrum, and hence on the calculated dose, of varying the anode angle, tube voltage, and total filtration of the x-ray tube is investigated. Codes used were those of Nowotny and Höfer (XCOMP), Boone, Iles, and Tucker et al. The code based on the work of Tucker et al. produced calculated doses noticeably lower than the other codes and compared best to the measured value. The variation in calculated dose between the Tucker code and the others is of the same order as the variation introduced by uncertainties in total filtration of about 20%, in peak tube voltage of +/- 4 kV, and in change of anode angle from 7 degrees to 13 degrees.

Biophysical Phenomena↗
PubMed1997

Human BSE.

Explore the source record for details and available documents.

Animals↗
PubMed1997

Hypnotherapy: complementary support in cancer care.

The psychological and physical consequences of cancer threaten patients' wellbeing and quality of life (Fallowfield 1991). Patients' needs are wide ranging and can include both personal and physical demands as well as support, relaxation and distraction. This article describes how many of these needs can be cared for by the skillful use of hypnotherapy.

Humans↗
PubMed1997

A comparison of radiation dose measured in CT dosimetry phantoms with calculations using EGS4 and voxel-based computational models.

CT is a high-dose examination and possibly the dominant contributor to dose from diagnostic radiology. Estimates of organ doses are obtained from Monte Carlo calculations and used to quantify radiation risk. To ensure the validity of using Monte Carlo calculations to estimate actual dose, measurements must be compared with calculations. We have measured doses to CT head and chest dosimetry phantoms and compared them with Monte Carlo (EGS4) calculated doses in voxel-based computational models of the phantoms. The simulation used an x-ray spectrum calculated from the specified values of the scanner's x-ray tube parameters. The scanner's beam-shaping filter was included in the modelling. Measured and calculated doses to both the head and chest phantoms agreed to within 7%. The inclusion of Rayleigh scattering in the calculations has a significant effect if only one slice is scanned but not if multiple slices are scanned.

Head↗
PubMed1997

Extent of antioxidant protection of plasma LDL is not a predictor of the antiatherogenic effect of antioxidants.

Oxidation of LDL plays all important role in atherogenesis. The lag-time in the formation of conjugated dienes provides a sensitive measure of the resistance of plasma LDL to oxidation and is widely assumed to be an indicator of atherogenic risk. To test this assumption, we investigated whether different antioxidants yielding similar lag-times result in similar reduction of atherosclerosis. A 6-months intervention study was carried out in three groups of 10 LDL receptor-deficient rabbits each. Because previous studies indicated that antioxidants that reduce atherosclerosis resulted in very long lag-times, the first group was treated with an antioxidant combination containing 1,000 IU vitamin E, 0.05% probucol analogue (BM15.0639), and 0.025% probucol. The lag-times achieved throughout the intervention period by this combination (952 +/- 39 min) were matched in a second group (829 +/- 46 min) treated with a variable dose of probucol (0.0575-0.11%, average 0.091%). A third, untreated group served as a control. Plasma cholesterol levels of all groups were matched. Even though both treatments yielded similar antioxidant protection of plasma LDL, 0.091% probucol reduced aortic atherosclerosis by 51.7% compared to the untreated group (P < 0.005), whereas the antioxidant combination failed to reduce lesion formation. Thus, the lag-time is clearly not correlated with the antiatherogenic efficacy of different antioxidants. However, a weak correlation was found within the group treated with probucol only. Our results suggest that the degree of antioxidant protection of plasma LDL may not be a good indicator of the atherogenic risk, in general, and that probucol reduces atherogenesis by mechanisms not shared by all antioxidants.

Animals↗
PubMed1994

RANTES chemokine expression in cell-mediated transplant rejection of the kidney.

RANTES (regulated upon activation, normal T cell expressed and secreted) is a chemotactic cytokine (a chemokine) for memory T lymphocytes, monocytes, and eosinophils. RANTES expression was studied in renal allograft biopsy specimens. Although RANTES was not expressed in samples taken one hour after transplantation, or in native renal biopsy specimens from patients with cyclosporin nephrotoxicity, it was expressed during cell-mediated transplant rejection. RANTES mRNA was detected in infiltrating mononuclear cells and renal tubular epithelium, and RANTES protein was localised to mononuclear cells, tubular epithelium, and vascular endothelium. This suggests RANTES has a role in allograft rejection.

Biopsy↗
PubMed1993

The parallel Lack anaesthetic breathing system.

The parallel Lack system is a new modification of the Mapleson A system comprising separate inspiratory and expiratory tubes. To determine that the function of the system was that anticipated of a Mapleson A, the fresh gas flow requirements to prevent rebreathing during spontaneous ventilation were assessed in three situations: (1) a lung model (2) conscious volunteers and (3) anaesthetised patients. Two sets of criteria to define rebreathing were used; (A) those based on changes in ventilation or end-expired carbon dioxide tension and (B) minimum inspired carbon dioxide tension. Using A, rebreathing occurred at a fresh gas flow to minute ventilation ratio (VF/VE) of 0.75 for the lung model, and 0.73 for conscious volunteers. These results were comparable to those obtained for a Magill attachment. They were also close to the point at which mechanical dead space began to increase in the lung model. Criteria B gave much lower values for the onset of rebreathing. Rebreathing was present by criteria A in five of the six anaesthetised patients at a fresh gas flow of 60 ml.kg-1.min-1 (VF/VF of 0.78). The results confirm that the parallel Lack behaves as a Mapleson A system. The resistance to breathing posed by the parallel Lack was also comparable to the Magill system.

Adult↗
PubMed1993

The additional work of breathing imposed by Mapleson A systems.

The additional work attributable to breathing through five Mapleson A anaesthetic breathing systems (Magill, Lack, Parallel Lack, Humphrey ADE and Enclosed Magill) was studied using a lung model. With all five systems, the additional work was found to be a function of fresh gas flow, respiratory flow as well as system geometry. Within the range of fresh gas flow and respiratory flow studied, the additional work ranged between 80 mJ.l-1 and 182 mJ.l-1. Expiratory work was always greater than the inspiratory workload. Increasing fresh gas inflow into the system increases expiratory work, both resistive and elastic components. The Magill system posed the least work expenditure. The values for the additional work obtained with the lung model were of the same order of magnitude when measurements were taken in volunteers.

Adult↗
PubMed1993

Characterization of a nested polymerase chain reaction assay for detection of parvovirus B19.

The characterization and application of a nested polymerase chain reaction (PCR) assay for the detection of human parvovirus B19 DNA is described. The assay was evaluated with 149 diagnostic serum samples (collected up to 150 days after the onset of symptoms) previously tested by dot blot hybridization for B19 DNA and by class-specific capture radioimmunoassays for the detection of B19 immunoglobulin M (IgM) and IgG. B19 DNA was detectable by the PCR in 70% of the sera. There was a statistically significant association between the detection of B19 DNA by PCR and high B19 IgM values (P < 0.005), low B19 IgG values (P < 0.05), and a short interval between onset of symptoms and serum collection (P < 0.005). Serial serum samples, throat swabs, and peripheral blood mononuclear cells collected from 10 individuals during an outbreak of parvovirus B19 were also tested by the nested PCR. B19 DNA was detectable in the throat swabs at the time of the clinical illness and in the peripheral blood mononuclear cell fraction up to the end point of the study 6 months after infection. The location of the B19 DNA could not be determined in cytocentrifuge preparations of peripheral blood mononuclear cells with nonisotopic in situ hybridization and immunolabelling.

Antibodies, Viral↗
PubMed1992

Pre-oxygenation: the Hudson mask as an alternative technique.

The use of a simple oxygen facemask (Hudson) with high oxygen inflow (48 l.min-1) was investigated as a technique for pre-oxygenation, comparing it with the Magill system (oxygen flow: 100 ml.kg-1.min-1). One hundred and thirty-eight patients scheduled for elective gynaecological and orthopaedic surgery were studied: group 1, Hudson mask and group 2, Magill system (ASA 1-2, n = 107); group 3, Hudson mask and group 4, Magill system (ASA 3, n = 30). Pre-oxygenation was assessed by measuring the times to 97%, 95% and 93% arterial desaturation (finger pulse oximetry) following 3 min of pre-oxygenation. The times taken to achieve these end-points in all the study groups suggest that the Hudson mask offers an alternative technique for pre-oxygenation.

Adult↗