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Biomedical subjects

J Partridge

Publications and source records attributed to J Partridge.

At least 37 records · Page 2Linked to original sources
PubMed1996

An evaluation of the impact of social interaction skills training for facially disfigured people.

Facially disfigured people can experience significant psychological problems, commonly relating to difficulties in social interaction. The effect of social interaction skills workshops on the psychological well-being of 64 facially disfigured participants is described. Participants completed the Hospital Anxiety and Depression Scale (HAD), the Social Avoidance and Distress Scale (SAD) and an open-ended questionnaire, before a workshop and at 6 weeks and 6 months follow-up. The high levels of anxiety evident prior to the workshop fell significantly 6 weeks post-workshop (HSD = 1.297, P < 0.01) and remained significantly lower at 6 month follow-up (HSD = 1.563, P < 0.01). Similarly, SAD scores fell significantly at 6 weeks (HSD = 1.89, P < 0.05) and again at 6 month follow-up (HSD = 2.26, P < 0.01). 6 weeks post-workshop, participants reported feeling more confident in the company of strangers (HSD = -1.266, P < 0.01) and about meeting new people (HSD = -1.159, P < 0.01). This increase in confidence was maintained at 6 months (HSD = -1.068 and -1.042 respectively, P < 0.01 for both). 61% of those who experienced problems before the workshop reported a positive change in these situations. The potential of these workshops as an addition to surgical intervention is discussed.

Adaptation, Psychological↗
PubMed1995

Effect of physician specialty on use of necessary coronary angiography.

OBJECTIVES: This study sought to determine whether having a cardiologist as a regular source of care influences likelihood of undergoing necessary coronary angiography. BACKGROUND: An important element of the current health policy debate is the respective roles of primary care and specialist physicians. However, there are few data on interspecialty differences in quality of care for patients with ischemic heart disease. METHODS: We contacted 243 patients by telephone (response rate 72%) who had positive (or very positive) exercise stress test results and met additional clinical criteria for necessary coronary angiography. Study patients were randomly sampled from those undergoing exercise stress testing at one university and three public hospitals in Los Angeles between January 1, 1990 and June 30, 1991. Patients were asked whether they had a regular source of care during the time after their exercise stress test and, if so, whether that provider was a cardiologist or cardiology clinic. RESULTS: Among survey responders, 47% underwent necessary coronary angiography within 3 months of exercise testing and 61% within 12 months. After adjustment for sociodemographics and clinical presentation, patients with a cardiologist as a regular source of care were more likely than all other patients to have undergone necessary angiography within 3 months (52% vs. 38%, p = 0.05) and within 12 months (74% vs. 44%, p = 0.0001) of the exercise test. At 3 months, there was a trend toward a more pronounced effect of ongoing cardiologic care within the public hospitals compared with the private hospital (p = 0.09 for interaction between hospital types). CONCLUSIONS: Patients with a cardiologist as a regular source of care were more likely than all other patients to undergo clinically necessary coronary angiography within both 3 and 12 months of exercise stress testing.

Cardiology↗
PubMed1995

Psychological and social aspects of burns.

Although the literature on the psychological consequences of burns is fairly extensive, the evidence gathered by the charity 'Changing Faces' over the last 2 years suggests that the resources allocated to psychological rehabilitation are still far from adequate. This article draws on the personal experience of one of the authors who was in a car fire 24 years ago, reviews some of the literature, briefly looks at the work done by Changing Faces and discusses some of the tasks of the 'burns team'. The authors are aware that generalizations about the consequences of burns are fraught with risk because every fire has its own causes and complications, but it is hoped that the themes expressed here do raise important general points.

Burns↗
PubMed1995

Simplified method for compression of femoral false aneurysms.

False aneurysm formation is a not uncommon complication of cardiac catheterization. Until recently, surgical repair was the only therapeutic option available when conservative management failed. However, Doppler-guided compression of the aneurysm has been advocated in recent years; the method requires prolonged indirect manual compression of the femoral artery aneurysm and is uncomfortable for both the patient and operator. In ten consecutive patients the use of Doppler-guided clamp placement and aneurysm compression for 60 min resulted in complete thrombosis of the aneurysm in eight, and only one patient required surgical repair. The procedure was well tolerated by all patients (analgesia was administered liberally). Eight patients were discharged within 24 h, one after 2 days and the single surgical patient remained an inpatient for 6 days. Doppler-guided clamp compression of false aneurysms is a safe, effective and cost effective method of managing these patients.

Aged↗
PubMed1994

A novel 13C isotopic labelling strategy for probing the structure and dynamics of glycan chains in situ on glycoproteins.

A protocol is described for uniform 13C labelling of terminal galactose residues of the glycan chains of glycoproteins, using an enzymatic method which does not perturb the protein. The technique is illustrated by application to the biantennary N-linked glycan chains attached at Asn 297 of immunoglobulin G (IgG). Isotope-edited NMR experiments on this glycoprotein yield data which suggest that the galactose residues on the glycan exist in two discrete environments, with the galactose in one environment having greater mobility than that in the other. These data are qualitatively consistent with crystallographic data on an Fc fragment, which suggest that one arm of the glycan is in contact with the protein, while the other projects into the space between the C gamma 2 domains. Quantitatively, however, these data cannot be rationalized with the crystallographic data, which implies subtle differences in oligosaccharide structure and dynamics between the solution and crystal states of Fc.

Carbohydrate Sequence↗
PubMed1993

Characterization of the extent of internal motions in oligosaccharides.

A detailed investigation has been undertaken on the extent and nature of torsional fluctuations about the glycosidic linkage of the model disaccharide Man alpha 1-3Man alpha 1-OMe. In particular, we sought to determine whether the three nuclear Overhauser effects and the two long-range heteronuclear 3JCH spin coupling constants measurable across the glycosidic linkage were consistent with a single conformation or multiple conformations about that linkage. Within experimental error, we have found that these five parameters can be interpreted in terms of a single, rigid geometry. Alternatively, the data are also consistent with a model in which the glycosidic torsional angles exhibit significant but restricted fluctuations about the global minimum energy conformation. Evidence from restrained molecular dynamics simulations both in vacuo and with explicit inclusion of solvent water and from 13C relaxation measurements upon an oligomannose glycan in covalent association with protein suggests that the latter model is the most accurate representation of the conformational behavior of oligosaccharides in solution.

Carbohydrate Conformation↗
PubMed1993

Nucleotide sequence analysis and heterologous expression of the Erysipelothrix rhusiopathiae dnaJ gene.

DNA sequence analysis of chromosomal DNA from the Gram-positive facultative intracellular pathogen, Erysipelothrix rhusiopathiae has identified a dnaJ heat shock gene homolog. A 1109-bp open reading frame encoding dnaJ is located immediately 3' to the E. rhusiopathiae dnaK gene. The deduced DnaJ amino acid sequence exhibits the modular structure of other members of the DnaJ protein class including a glycine-rich region and the repeating consensus sequence CXXCXGXGX. Heterologous expression of the dnaJ sequence in Escherichia coli resulted in accumulation of a unique 38.9-kDa protein with an isoelectric point of 8.0. Deletion analysis of the dnaJ gene was used to confirm that the overproduced protein was encoded by the dnaJ sequence.

Amino Acid Sequence↗
PubMed1993

Cloning, heterologous expression, and characterization of the Erysipelothrix rhusiopathiae DnaK protein.

The dnaK (hsp70) gene from the facultative intracellular pathogen Erysipelothrix rhusiopathiae was cloned by heterologous DNA hybridization of a genomic library using the Escherichia coli dnaK gene as a probe. A 3.2-kb fragment which encoded an 1,800-bp open reading frame was recovered. The deduced amino acid sequence of this open reading frame shares 56% identity with the E. coli DnaK protein. Expression of the encoded protein in E. coli by using the phage T7 promoter/polymerase system resulted in accumulation of a unique 65-kDa protein. Western blot (immunoblot) analysis of extracts from a recombinant E. coli strain using anti-E. coli DnaK polyclonal antibodies confirmed that the cloned gene encodes a DnaK homolog. The recombinant E. rhusiopathiae DnaK protein was purified to 80% homogeneity by ATP affinity chromatography. The purified material hydrolyzed ATP with a specific activity of 100 nmol min-1 mg of protein-1. Analysis of total protein extracts from E. rhusiopathiae indicates that DnaK is a highly expressed protein in this organism.

Adenosine Triphosphate↗
PubMed1990

The acute abdomen.

Explore the source record for details and available documents.

Abdomen, Acute↗
PubMed1986

The plain chest radiograph in uncomplicated Ebstein's disease.

Displacement of the origin of the tricuspid valve into the right ventricle, redundancy of its leaflets and tricuspid incompetence are all present to varying degrees in Ebstein's disease. Cardiac arrhythmias are a common feature. Presentation in infancy with severe displacement of the valve, right-sided heart failure, cyanosis and gross cardiomegaly are the classic features. More recently, echocardiography has revealed a group presenting with arrhythmias but with no significant haemodynamic disturbance, or with an asymptomatic murmur who have a normal or near normal chest radiograph and mild displacement of the valve. Before the availability of echocardiography, most of this group escaped diagnosis as there were insufficient indications for cardiac catheterisation.

Adolescent↗