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Biomedical subjects

J Paluszak

Publications and source records attributed to J Paluszak.

66 records · Page 4Linked to original sources
PubMedDate not supplied

Effect of 2-deoxy-D-glucose on lipolytic processes in blood and adipose tissue of rat.

The effect of 2-deoxy-D-glucose on lipolytic processes in the blood and adipose tissue was studied. Rats treated with this antimetabolite showed a significant increase in serum glucose, FFA and glycerol level, as well as in the lipid mobilizing activity. On the other hand, the lipolytic activity of rat serum decreased when compared to control group. From these results it may be concluded that during hypothermia induced by administration of 2-deoxy-D-glucose intracellular, but not intravascular, lipolysis is enhanced.

Adipose Tissue↗
PubMedDate not supplied

Ledakrin effect on free fatty acid and glycerol mobilization from epididymal adipose tissue of rat in vitro.

It was demonstrated that Ledakrin, an antitumour drug, causes mobilization of free fatty acids and glycerol from the epididymal adipose tissue of rat in vitro. The disproportion observed in the release of glycerol and free fatty acids following Ledakrin administration suggested a biphasic effect of this drug, with initial stimulation and later inhibition of the processes of lipolysis and lipogenesis. Blockade of adrenergic membrane receptors (with propranolol or regitine) abolished the lipolytic effect of Ledakrin.

Acridines↗
PubMedDate not supplied

Effects of insulin and beta-adrenergic blockade on certain indicators of carbohydrate metabolism in the blood and tissues of rats during short-lasting hypothermia.

Administration of insulin 1 i.u./100 g of body weight to hypothermic rats causes a fall of glucose and lactate levels in the serum and a rise in myocardial glycogen level in relation to the group of control rats kept at room temperature and to the group of rats subjected only to hypothermia. Beta-adrenergic blockade (propranolol 0.6-1 mg/kg) caused no changes in the levels of carbohydrate metabolites in the serum of hypothermic rats but raised the myocardial glycogen level by 42% in relation to the animals subjected only to hypothermia. Simultaneous administration of both these agents during hypothermia produces a fall of the serum levels of glucose and pyruvate with a rise in the level of lactate, and raises the glycogen level in the myocardium (by about 161%) and in the skeletal muscle (by 54%) in relation to the rats subjected to hypothermia alone. Insulin and/or propranolol fail to prevent glycogen reserve exhaustion in the liver of hypothermic rats which could be due to activation of non-blocked alpha-adrenergic receptors or to the action of yet another glycogenolytic agent, e.g. glucagon, during hypothermia.

Acute Disease↗
PubMedDate not supplied

Somatostatin effect on the level of adenyl nucleotides in the blood and tissues of rats during short-lasting hypothermia.

Short-lasting hypothermia during thiobutabarbital general anaesthesia causes no decrease of the absolute ATP level in the blood and liver of rats. The adenylate energy charge in the tissues is relatively high - 0.86 in the liver and 0.85 in the muscles, which might be an evidence of a significant "energy sparing" during moderate hypothermia (26 +/- 1 degree C). Somatostatin in a dose of 20 micrograms/kg of body weight given to the rats during hypothermia decreased the ATP level, the ATP/ADP ratio and the adenylate energy charge in the studied tissues, especially in the liver, evidencing increased intensity of catabolic processes caused by the inhibitory action of somatostatin on the release of insulin and glucagon, among other hormones, and on the change of the insulin/glucagon ratio.

Adenosine Diphosphate↗
PubMedDate not supplied

Effect of certain factors modifying the lipid-mobilizing activity in vitro in the serum of rats during hypothermia.

The serum of hypothermic animals shows in vitro the ability of mobilizing fatty acids from the adipose tissue. This ability is quantitatively comparable to the lipolytic activity of glucagon but is lower than that of adrenaline. Propranolol decreases the lipid-mobilizing activity in the serum of hypothermic animals in vitro. Similarly insulin added to the medium with the serum of hypothermic animals decreased the lipid-mobilizing activity.

Adipose Tissue↗
PubMedDate not supplied

In vitro serum glycogenolytic activity in rats during hypothermia induced with 2-deoxy-D-glucose.

During hypothermia induced by intraperitoneal administration of 2-deoxy-D-glucose (600 mg/kg of body weight) the serum levels of glucose and FFA rise and the hepatic glycogen content falls in relation to rats in control group. The glycogenolytic activity of the serum in vitro determined against liver slices is also higher in the group of rats receiving 2-DG. The obtained results point to an activation of the glycogenolysis process and glycolysis in the organism of rats after administration of hypothermia-inducing doses of 2-DG.

Animals↗
PubMedDate not supplied

Effect of hyperthermia on blood levels of corticosterone and certain metabolites in rats during thiobutabarbital anaesthesia.

No changes were found in the serum levels of corticosterone, pyruvate and lactate in rats during general anaesthesia with thiobutabarbital (Brevinarcon) subjected to short-lasting hyperthermia in a high-temperature chamber (air temperature 50 degrees C, relative humidity 50%) in relation to a control group of rats during similar general anaesthesia at room temperature. However, in the serum of rats during hyperthermia (rectal temperature 40-41 degrees C) the glucose level was about 52% lower and FFA were about 39% lower than in rats kept under normothermic conditions (rectal temperature 36.5-37.5 degrees C) which may point to an increased requirement of tissues for energy-yielding substrates at higher body temperatures and/or increased insulin secretion.

Anesthesia↗
PubMedDate not supplied

Activity of certain enzymes in the mitochondrial and cytoplasmic fractions of liver cells, myocardium and skeletal muscle of the rat during short-lasting hypothermia.

During short-lasting hypothermia in rats the activity of isocitrate dehydrogenase (E.C.1.1.1.42) and malic dehydrogenase (E.C.1.1.1.37) was determined in the mitochondrial fraction of liver cells, myocardial cells and skeletal muscle fibres of the femoral muscle and no statistically significant differences were found between the obtained values and those in a control group. On the other hand, the activity of glucose-6-phosphate dehydrogenase (E.C.1.1.1.49) in the cytoplasmic fraction of these tissues was decreased in the hypothermic rats by 46% in the liver, by 50% in the myocardium and by 59% in the skeletal muscle of the thigh. A possible cause of this reduced activity of this enzyme in hypothermia could be changes in the structure of the enzymatic protein, deficiency of the hormone activating the enzyme, that is insulin, and/or inhibition of the pentose-phosphate cycle by fatty acids released in excess.

Animals↗
PubMedDate not supplied

Ledacrine effect on free fatty acids and glycerol mobilization from rat adipose tissue in vivo.

In rats receiving intraperitoneally Ledacrine in toxic doses the free fatty acids (FFA) and glycerol serum levels and the amount of fatty acids and glycerol released from the epididymal tissue of the rats were determined during one-hour incubation in vitro. It was found that Ledacrine in toxic doses stimulated the process of lipolysis as evidenced by raised serum FFA and glycerol levels and increased amount of FFA and glycerol released from the epididymal adipose tissue in vitro in relation to the values in control rats. The results of investigations of acid-base equilibrium parameters indicated that Ledacrine in toxic doses caused in rats metabolic acidosis associated with respiratory acidosis.

Acid-Base Equilibrium↗