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Biomedical subjects

J P Mitchell

Publications and source records attributed to J P Mitchell.

At least 73 records · Page 4Linked to original sources
PubMed1978

Transfer of adoptive immunity by intra-arterial injection of tumor-immune pig lymph node cells: treatment of recurrent urinary bladder carcinoma after radical radiotherapy.

Twenty-four patients with invasive transitional cell carcinoma of the urinary bladder, recurrent after radiotherapy, were treated by intra-arterial infusion of tumor-immune pig lymph node cells. In 11 patients there was a remission of the disease process, and 3 patients remain alive and free of disease in excess of one year after treatment.

Aged↗
PubMed1978

Mesectodermal leiomyosarcoma of the antrum and orbit.

A 39-year-old black man developed a left antral leiomyosarcoma that subsequently extended into the ipsilateral orbit. On light microscopic appearance of the antral tumor the presence of a distinctive myxoid and fibrillar cytoplasmic background suggested the diagnosis of a malignant Schwann's cell tumor. A second biopsy of the orbital extension displayed the same fibrillar character, but the emergence of tumor giant cells with cytoplasmic trichrome positivity also raised the possibility of a rhabdomyosarcoma. Electron microscopy demonstrated the smooth muscle derivation of the tumor, which probably originated from vascular smooth muscle elements. The atypical neural appearance of this myogenous tumor may have been caused by the extensive neural crest contribution to the cephalic connective tissues (mesectoderm). Radiation therapy and chemotherapy were ineffective.

Adult↗
PubMed1978

Carcinoma of the penis.

Sixty-three patients with squamous cell carcinoma of the penis have been reviewed. The 3 year survival was 77%, and the 5 year survival was 59%. Prognosis was related to both clinical staging and histological grading. Stage I and II carcinomas were best treated by surgery alone. Stage III and IV carcinomas were best treated by local amputation of the penis combined with radiotherapy to the inguinal nodes. Surgery as a primary treatment for clinically positive nodes was successful in less than half the cases treated, and in this series was always followed by wound infection and lymphoedema. Tender, enlarged inguinal lymph nodes should be observed for up to 3 months following primary treatment, as a large percentage of these nodes are inflammatory and subside spontaneously.

Adult↗
PubMed1978

Adoptive immunotherapy and radiotherapy in the treatment of urinary bladder cancer.

Thirty-one patients with invasive transitional cell carcinoma of the urinary bladder were treated either by infusion of tumour immune pig mesenteric lymph node cells into the tumour blood supply, followed 6 weeks later by 5500 rad, or by radiotherapy alone. The patients were paired prior to treatment, using 4 criteria: age (nearest decade), sex, clinical stage (T3 or T4) and histological grade (average or high). The first patient in each pair then received combined treatment and the second radiotherapy alone. Administration of pig cells before radiotherapy offered no advantage to the patient in terms of the incidence of remission or the length of survival after treatment.

Animals↗
PubMed1977

Model system studies of staining procedures for lysine and arginine residues.

Using polyacrylamide films containg poly-lysine, polyarginine and DNA as test models, a variety of reportedly specific staining procedures have been examine. Contrary to published observations, mixtures of fast green and eosin Y show no specific staining of either lysine or arginine. Both amino-acids bind eosin from the mixture more strongly than fast green. Arginine apparently has a greater affinity for this eosin than has lysine which contradicts previous reports that lysine will be stained by eosin arginine will stain with fast green, if proteins containing both amino-acids are stained with dye mixture. In films containing lysine and/or arginine picric acid is shown to bind specifically to the arginine. The picric acidarginine complex resists disruption in 0.004 M borate buffer which is a solvent used for subsequent staining of lysine residues with bromophenol blue. Picric acid may also be used as a hydrolysant and substitute for hydrocholoric acid in a Feulgen-like procedure which stains DNA to the same level as the classiclal hydrochloric acid based procedure while also staining arginine present.

Arginine↗
PubMed1975

Combined staining of protein-bound sulphydryl groups and DNA in polyacrylamide model systems.

A model system of polyacrylamide films containing protein and DNA has been used to examine the feasibility of combining the dihydroxydinaphthyldisulphide (DDD)-diazonium salt procedure for localizing protein-bound sulphydryl groups with the Feulgen technique for DNA to make possible the direct measurement of both these parameters simultaneously. Optimun conditions for the sulphydryl group reaction require reduction of the protein-containing films in 10% aqueous ammonium sulphide for 3 hr at 50 degree C followed by treatment with a DDD solution at 50 degree C for 4 hr. The final coloured product was developed in a solution of the diazonium salt, Fast Red TR, for 15 min. The azo compound thus produced was completely resistant to hydrochloric acid hydrolysis in the manner of the Feulgen reaction. Calculation of protein-bound sulphydryl groups and DNA from measurements made on doubly-stained films showed excellent agreement between the measured and the expected values.

Acrylamides↗
PubMed1975

Trauma to the urethra.

Little is known of the exact aetiology of traumatic stricture of the urethra, but the first clinical presentation can be delayed many years after the trauma. Diagnosis can be made on clinical grounds alone and the passage of a catheter is condemned as clinically misleading and surgically dangerous. Partial rupture is relatively common and every effort should be made to preseve the remaining bridge of tissue which may still be viable. Initial treatment should ensure that no additional damage is done to the urethra by converting a partial rupture into a complete transection.

Humans↗