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J P Kilbourn

Publications and source records attributed to J P Kilbourn.

At least 19 recordsLinked to original sources
PubMed1993

Immunoreactive calmodulin in cystic fibrosis kidneys.

BACKGROUND: Microscopic nephrocalcinosis has been detected at autopsy in 35 of 38 (92%) cystic fibrosis (CF) patients. A primary defect of calcium metabolism in the kidney has been postulated. Intracellular calcium and calmodulin (a calcium binding protein) have been shown to be increased in CF tissues. AIMS: To determine whether CF kidneys obtained at autopsy contain normal amounts of calmodulin and whether the presence of immunoreactive calmodulin correlates with nephrocalcinosis. METHODS: An immunoperoxidase method was used to stain kidneys obtained from six CF individuals aged three to 27.5 years (mean 15.75 +/- 10.2 years) and five individuals who died of chronic debilitating disease aged eight to 17 years (mean 13.6 +/- 3.9 years). Calcium staining was undertaken using the von Kossa (silver precipitation) and Alizarin red methods. RESULTS: No quantifiable difference in immunoreactive calmodulin staining was demonstrated in autopsy renal specimens between six CF patients and five chronically diseased controls. Three of six CF patients aged 22.8 +/- four years and two of five chronically diseased controls aged eight to 16 years respectively had detectable immunoreactive calmodulin staining. Within the CF group the presence of microscopic nephrocalcinosis did not correlate with immunoreactive calmodulin staining. CONCLUSION: This study does not support the premise that a calmodulin-dependent defect of intracellular calcium handling contributes to the microscopic nephrocalcinosis seen in CF.

Adolescent↗
PubMed1986

Demonstration of calcitonin and calmodulin by immunoperoxidase in the cystic fibrosis lung.

In this investigation, the presence of calcitonin and calmodulin has been demonstrated in immunoperoxidase formalin-fixed, paraffin-embedded sections of lung from autopsy tissues of four patients who died as a result of complications resulting from their cystic fibrosis disease. Immunoreactive calcitonin has been stained and quantitated in solitary endocrine cells which are increased in number and staining intensity in cystic fibrosis lung when compared to COPD and normal lungs. Immunoreactive calmodulin has been demonstrated to be increased in cystic fibrosis lung when compared to COPD and normal lungs. Previously, increased calcitonin and calmodulin were identified in sputum from cystic fibrosis patients utilizing radioimmunoassay. The calcitonin and calmodulin may be associated with increased calcium in pulmonary secretions leading to selective colonization of the lung by a limited number of pathogenic bacteria and enhanced pulmonary infection.

Calcitonin↗
PubMed1983

Hemophilus influenzae biotypes and chronic bronchitis.

Hemophilus influenzae can be differentiated into 6 biotypes on the basis of 3 biochemical reactions. This study was undertaken to determine the biotypes of respiratory isolates from an adult population with chronic bronchitis and to compare the biotypes of upper and lower respiratory tract isolates of individual patients. For rapid biotyping of serologically nontypable H. influenzae, we used 2 commercially prepared kits (Minitek and IDS RapID Systems). Twenty-three of 29 isolates (79%) had biochemical characteristics of biotype II with the Minitek system. Eighteen of the same 29 isolates were retested with the IDS RapID System and reacted as biotype II organisms. Seven of 9 and 4 of 4 paired transtracheal/oropharyngeal samples had identical biotypes when tested with the Minitek and IDS RapID System, respectively. These findings suggest that H. influenzae organisms are exchanged between the oropharynx and tracheobronchial tree of elderly male patients with chronic bronchitis.

Bronchitis↗
PubMed1979

Reproducibility of biotypes using enterotubes.

Using both stock and clinical isolates, the Enterotube system was examined for reproducibility of its biotype identification. Fifty stock strains of Enterobacteriaceae were tested on each of 22 separate days (interrun evaluation), and ten Enterotubes per strain were inoculated from one subculture (intrarun evaluation). It was found that 2 of the 50 strains (4 percent) demonstrated reproducibility of biotypes, defined as no variation in the results of the 22 interrun Enterotubes. Twenty-one of the 50 strains (42 percent) demonstrated reproducibility of biotypes, defined as no variation in the results of the ten intrarun Enterotubes. In addition, 20 replicate Enterotube biotype identifications were performed on each of 114 urine cultures which had greater than 10(5) morphologically identical colonies per milliliter of urine. Forty-two of these 114 clinical urine cultures (34 percent) demonstrated no variation in biochemical test results with the 20 replicate Enterotubes. In 47 (44 percent) of the 20 replicate urine cultures, one biochemical test result varied. Two or more biochemical test results were different in the 20 Enterotubes from 25 (22 percent) of the urine cultures.

Bacteriological Techniques↗
PubMed1977

Oxi-fermTM system.

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Bacteriological Techniques↗